研究报告

Mapping QTLs for Drought Resistance at Seedling Stage in Rice by Using a Doubled Haploid Population

Expand
  • State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; *Corresponding author, E-mail: xwei@mail.hz.zj.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2010-09-10

Abstract

Abstract: The QTLs for drought resistance at the seedling stage were analyzed by using a doubled haploid (DH) population consisted of 251 lines from the cross between a japonica parent Maybelle and an indica parent Baiyeqiu. A genetic linkage map with 226 SSR markers was constructed. Through singlelocus analysis following composite interval mapping (CIM), a total of 5 QTLs were detected on 5 different chromosomes. Four QTLs were also detected by twolocus analysis, which resolved 4 epistatic QTLs (EQTLs). The additive genetic effects of all QTLs were positive, indicating the alleles from Baiyeqiu improve drought tolerance of the DH population.

Cite this article

XU Qun,YUAN Xiao-ping,YU Han-yong,WANG Yi-ping,TANG Sheng-xiang,WEI Xing-hua* . Mapping QTLs for Drought Resistance at Seedling Stage in Rice by Using a Doubled Haploid Population[J]. Chinese Journal OF Rice Science, 2010 , 24(5) : 469 -473 . DOI: 10.3969/j.issn.1001-7216.2010.05.004

References

[1]Maclean J L, Dawe D C, Hardy B, et al. Rice Almanac. 3rd ed. Los Banos, Philippines: IRRI, 2002.
[2]余守武, 谢建坤, 万勇, 等. 水稻抗旱性相关性状的QTLs定位研究进展. 分子植物育种, 2004, 2(3): 391-400.
[3]Price A H, Tomos A D, Virk D S. Genetic dissection of root growth in rice (Oryza sativa L.): Ⅰ. A hydroponic screen. Theor Appl Genet, 1997, 95: 132-142.
[4]Yadav R, Courtois B, Huang N, et al. Mapping genes controlling root morphology and root distribution in a double-haploid population of rice. Theor Appl Genet, 1997, 94: 619-632.
[5]Hemamalini G S, Shashidhar H E, Hittalmani S. Molecular marker assisted tagging of morphological and physiological traits under two contrasting moisture regimes at peak vegetative stage in rice (Oryza sativa L.). Euphytica, 2000, 112: 69-78.
[6]Courtois B, Shen L, Petalcorin W, et al. Locating QTLs controlling constitutive root traits in the rice population IAC 165×Co39. Euphytica, 2003, 134: 335-345.
[7]Nguyen T T T, Klueva N, Chamareck V, et al. Saturation mapping of QTL regions and identification of putative candidate genes for drought tolerance in rice. Mol Gen Genomics, 2004, 272: 35-46.
[8]Price A H, Steele K A, Moore B J, et al. A combined RFLP and AFLP linkage map of upland rice (Oryza sativa L.) used to identify QTLs for root-penetration ability. Theor Appl Genet, 2000, 100: 49-56.
[9]Ray J D, Yu L, McCouch S R, et al. Mapping quantitative trait loci associated with root penetration ability in rice (Oryza sativa L.). Theor Appl Genet, 1996, 92: 627-636.
[10]Ali M L, Pathan M S, Zhang J, et al. Mapping QTLs for root traits in a recombinant inbred population from two indica ecotypes in rice. Theor Appl Genet, 2000, 101: 756-766.
[11]徐吉臣, 李晶昭, 郑先武, 等. 苗期水稻根部性状的QTL定位. 遗传学报, 2001, 28(5): 433-438.
[12]Zhang J, Zheng H G, Aarti A, et al. Locating genomic region associated with components of drought resistance in rice: Comparative mapping within and across species. Theor Appl Genet, 2001, 103: 19-29.
[13]Tripathy J N, Zhang J, Robin S, et al. QTLs for cell-membrane stability mapped in rice (Oryza sativa L.) under drought stress. Theor Appl Genet, 2000, 100: 1197-1202.
[14]Champoux M C, Wang G, Sarkarung S, et al. Locating genes associated with root morphology and drought avoidance in rice via linkage to RFLP markers. Theor Appl Genet, 1995, 90: 969-981.
[15]滕胜, 钱前, 曾大力, 等. 水稻苗期耐旱性基因位点及其互作的分析. 遗传学报, 2002, 29(3): 235-240.
[16]Zhang X , Zhou S X, Fu Y C, et al. Identification of a drought tolerant introgression line derived from Dongxiang common wild rice (O. rufipogon Griff.). Plant Mol Biol, 2006, 62: 247-259.
[17]Shen L, Courtois B, McNally K L, et al. Evaluation of near-isogenic lines of rice introgressed with QTLs for root depth through marker-aided selection. Theor Appl Genet, 2001, 103(1): 75-83.
[18]Steele K A, Price A H, Sashidhar H E, et al. Marker-assisted selection to introgress rice QTLs controlling root traits into an Indian upland rice variety. Theor Appl Genet, 2006, 112: 208-221.
[19]Yang J, Zhu J, Williams R W. Mapping the genetic architecture of complex traits in experimental populations. Bioinformatics, 2007, 23: 1527-1536.
[20]Adam P, Brigitte C. Mapping QTLs associated with drought resistance in rice: Progress, problems and prospects. Plant Growth Regul, 1999, 29: 123-133.
[21]Courtois B, Ahmadi N, Khowaja F, et al. Rice root genetic architecture: Meta-analysis from a drought QTL database. Rice, 2009, 2(2/3): 115-128.
[22]Redona E D, Mackill D J. Mapping quantitative trait loci for seedling vigor in rice using RFLPs. Theor Appl Genet, 1996, 92: 395-402.
[23]Zeng H Z, Zhong Y, Luo L J. Drought tolerance genes in rice. Funct Integr Genom, 2006, 6: 338-341.
[24]王贺正, 李艳, 马均, 等. 水稻苗期抗旱性指标的筛选. 作物学报, 2007, 33(9): 1523-1529.
[25]Price A H, Tomos A D. Genetic dissection of root growth in rice (Oryza sativa L.):Ⅱ. Mapping quantitative trait loci using molecular markers. Theor Appl Genet, 1997, 95: 143-152.
[26]郑天清, 徐建龙, 傅彬英, 等. 回交高代选择导入系的纹枯病抗性与抗旱性的遗传重叠研究. 作物学报, 2007, 33(8): 1380-1384.
Outlines

/

Tel: 0571-63370278 E-mail: cjrs@263.net
Supported by Beijing Magtech Co., Ltd.