Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2010 , Vol. 24 >Issue 4: 391 - 397

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.04.010

研究报告

Effect of H2O2 Pretreatment on Cd Tolerance of Different Rice Cultivars

Expand
  • 1College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China; 2Life Science Experimental Center, Nanjing Agricultural University, Nanjing 210095, China; 3 Jiaxing Academy of Agricultural Sciences, Jiaxing 314016, China; *Corresponding author, E-mail: yingge711@njau.edu.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2010-07-10

Fold

Abstract

The effect of H2O2 pretreatment on Cd tolerance and translocation in rice seedlings was studied by using two rice cultivars (N076 and N0763) differing in Cd tolerance. The contents of malondialdehyde(MDA), reduced glutathione (GSH), nonprotein thiols (NPT), phytochelatins(PCs) and the activity of glutathione Stransferase (GST) were compared between the two cultivars exposed to various treatments. The results showed that 50 μmol/L Cd exposure significantly inhibited rice growth, enhanced the production of GSH, NPT, PCs, MDA and the activity of GST, and there were significant differences between the two cultivars. More Cd was transported into the shoot in N076. The H2O2 pretreatment alleviated Cd toxcity by further increasing GSH, NPT and PCs contents, as well as the root GST activities. The larger increase in N0763 than in N076 suggests that the tolerance of N0763 was enhanced more significantly than N076. H2O2 pretreatment reduced Cd translocation to rice shoots but affected the Cd concentration in roots differently. From the above results, it may be speculated that there were clear differences in the Cd detoxification and response to the H2O2 pretreatment between the two cultivars.

Key words： cadmium; hydrogen peroxide; pretreatment; rice; tolerance

Cite this article

BAI Xiao-juan,LIU Li-juan,ZHANG Chun-hua,GE Ying,CHENG Wang-da . Effect of H2O2 Pretreatment on Cd Tolerance of Different Rice Cultivars[J]. Chinese Journal OF Rice Science, 2010 , 24(4) : 391 -397 . DOI: 10.3969/j.issn.1001-7216.2010.04.010

References

[1]Schützendübel A, Polle A. Plant response to abiotic stresses: Heavy metal-induced oxidative stress and protection by mycorrhization. J Exp Bot, 2002, 372(53): 1351-1365.
[2]Schützendübel A, Schwanz P, Teichmann T, et al. Cadmium induced changes in antioxidative systems, hydrogen peroxide content, and differentiation in scots pine roots. Plant Physiol, 2001, 127: 887-898.
[3]Zenk M H. Heavy metal detoxification in higher plants： A review. Gene, 1996, 179: 21-30.
[4]Mehra R K, Tripathi R D. Phytochelatins and metal tolerance//Agrawal S B, Agrawal M. Environmental Pollution Plant Responses. Boca Raton, FL:CRC Press, Lewis Publisher, 1999: 367-382.
[5]Strasdeit H, Duhme A K, Kneer R, et al. Evidence for discrete Cd(SCys)4 units in cadmium phytochelatin complexes from EXAFS spectroscopy. J Chem Soc, Chem Commun, 1991, 16: 1129-1130.
[6]Tommasini R, Vogt E, Fromenteau M, et al. An ABC-transporter of Arabidopsis thaliana has both glutathione-conjugate and chlorophyll catabolite transport activity. Plant J, 1998, 13: 773-780.
[7]Rea P. MRP subfamily ABC transports from plants and yeast. J Exp Bot, 1999, 50: 895-913.
[8]Marrs K A. The functions and regulation of glutathione S-transferases in plants. Annu Rev Plant Physiol Plant Mol Biol, 1996, 47: 127-158.
[9]Dat J F, Vandendede F, Vranova E, et al. Dual action of the active oxygen species during plant stress response. Cells Mol Life, 2000, 57: 779-795.
[10]Overmyer K, Brosche H, Kangasjarvi J. Reactive oxygen species and hormonal control of cell death. Trends Plant Sci, 2003, 8: 335-342.
[11]Hung S H, Yu C W, Lin C H. Hydrogen peroxide functions as a stress signal in plants. Bot Bull Acad Sin, 2005, 46: 1-10.
[12]Robert B, David O A. Arabidopsis OXS2 is a transcription factor in the oxidative stress response//Abstract of annual meeting of the American Society of Plant Biologists, July 24-28, 2004, Orlando, FL, USA[EB/OL].[2009-08-29]. http://abstracts.aspb.org/pb2004/public/M04/9154.html.
[13]Shah K, Dubey R S. Effect of cadmium on RNA level as well as activity and molecular forms of ribonuclease in growing rice seedlings. Plant Physiol Biochem, 1995, 33: 577-584.
[14]Ge C L, Wang Z G, Wan D Z, et al. Proteomic study for response to cadmium stress in rice seedlings. Rice Sci, 2009, 16(1): 33-44.
[15]Hu Y L, Ge Y, Zhang C H, et al. Cadmium toxicity and translocation in rice seedlings are reduced by hydrogen peroxide pretreatment. Plant Growth Regul, 2009, 59: 51-61.
[16]Rama D S, Prasad M N V. Copper toxicity in Ceratophyllum demersum L. (Coontail) , a free floating macrophyte: Response of antioxidant enzymes and antioxidants. Plant Sci, 1998, 138: 157-165.
[17]Hissin P J, Hilf R. A fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem, 1976, 74(1): 215-226.
[18]Bhargava P, Srivastava A K, Urmil S, et al. Phytochelatin plays a role in UV-B tolerance in N2-fixing cyanobacterium Anabaena doliolum. Plant Physiol, 2005, 162: 1220-1225.
[19]孙琴, 袁信芳, 王晓蓉．环境因子对小麦体内镉的生物毒性和植物络合素合成的影响．应用生态学报, 2005, 16(7): 1360-1365．
[20]孔祥生, 易现峰．植物生理学实验技术.北京: 中国农业出版社, 2008．
[21]Habig W H, Pabst M J, Jakoby W B. Glutathione S-transferases: The first enzymatic step in mercapturic acid formation. J Biol Chem, 1974, 249(22): 7130-7139.
[22]Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 1976, 72: 248-254.
[23]Metwally A, Finkemeier I, Georgi M, et al. Salicylic acid alleviates the cadmium toxicity in barley seedlings. Plant Physiol, 2003, 132: 272-281.
[24]苗雨晨, 董发才, 宋纯鹏．过氧化氢-植物体内的一种信号分子．生物学杂志, 2001, 18(2): 4-6．
[25]Pnuel I L, Liang H, Rozenber G M, et al. Growth suppression, altered stomatal response, and augmented induction of heat shock protein in cytosolic ascorbate peroxidase (Apx1)-deficient Arabidopsis plants. Plant J, 2003, 34(2): 187-203.
[26]宋东亮, 沈君辉, 李来庚．高等植物细胞壁中纤维素的合成．植物生理学通讯, 2008, 44(4): 791-796．
[27]Domínguez-Solís J R, Gutiérrez-Alcalá G, Romero L C, et al. The cytosolic O-acetylserine(thiol)lyase gene is regulated by heavy metals and can function in cadmium tolerance. J Biol Chem, 2001, 276: 9297-9302.
[28]娄来清, 沈振国．金属硫蛋白和植物螯合肽在植物重金属耐性中的作用．生物学杂志, 2001, 18(3): 1-4．
[29]Guo B, Liang Y C, Zhu Y G. Does salicylic acid regulate antioxidant defense system, cell death, cadmium uptake and partitioning to acquire cadmium tolerance in rice. J Plant Physiol, 2009, 166(1): 20-31.
[30]Zhang C H, Ge Y. Response of glutathione and glutathione S-transferase in rice seedlings exposed to cadmium stress. Rice Sci, 2008, 15(1): 73-76.
[31]Adamis P D B, Gomes D S, Pinto M L C C, et al. The role of glutathione transferases in cadmium stress.Toxicol Lett, 2004, 154 (1/2): 81-88.
[32]Iannelli M A, Pietrine F, Fiore L, et al. Antioxidant response to cadmium in Phragmites australis plants. Plant Physiol Biochem, 2002, 40(11): 977-982.
[33]Levine A, Tenhaken R, Dixon R, et al. H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell, 1994, 79: 583-593.
Options
Outlines

/

〈 〉

Tel: 0571-63370278　E-mail: cjrs@263.net
Supported by Beijing Magtech Co., Ltd.