Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2010 , Vol. 24 >Issue 2: 131 - 136

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.02.05

研究报告

Comparative Analysis of Genomes of Oryza alta and Oryza latifolia by Using C0t-1 DNA

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  • Key Laboratory of State Ethnic Affairs Commission for Biological Technology/College of Life Sciences, SouthCentral University for Nationalities, Wuhan 430074, China；*Corresponding author, E-mail: liuhong2007@yahoo.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2010-03-10

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Abstract

In order to reveal the origin and evolutionary relationship between two CCDD genome species of rice, Oryza alta and Oryza latifolia, fluorescence in situ hybridization (FISH) procedure was adopted to analyse the genomes of O. alta and O. latifolia using C0t1 DNA from O. alta as a probe. Karyotype analysis between O. alta and O. latifolia was also made comparatively based on their similar band patterns of the hybridization signals. There was a high homology and close relationship between O. alta and O. latifolia, but at the same time, the distinction between the hybridization signals was also clear. This indicates that C0t1 DNA was species and genome typespecific, and C0t1 DNAFISH could be more efficient in analysis of the genome relationship between different species. According to the comparative analysis of highly and moderately repetitive DNA sequences between the two allotetraploidy species, O. alta and O.latifolia, the possible origin and evolutionary mechanism of allotetraploidy of Oryza were discussed.

Key words： Oryza alta; Oryza latifolia; chromosomal repeat sequence; fluorescence in situ hybridization; karyotype; genome; phylogenetic relation

Cite this article

WANG Debin, WANG Yang,ZHAO Houming, LI Gang, QIN Rui,LIU Hong* . Comparative Analysis of Genomes of Oryza alta and Oryza latifolia by Using C0t-1 DNA [J]. Chinese Journal OF Rice Science, 2010 , 24(2) : 131 -136 . DOI: 10.3969/j.issn.1001-7216.2010.02.05

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