Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2010 , Vol. 24 >Issue 1: 93 - 98

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.01.16

实验技术

Improvement of Method for Evaluating Amylose Content in Rice at Low Generations of Breeding

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  • 1State Key Laboratory of Rice Biology, China National Rice Research Institute， Hangzhou 310006， China； 2Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; *Corresponding author, E-mail: ricezxd@126.com

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2010-01-10

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Abstract

A simple iodine staining method for evaluating amylose content in rice was developed. Amylose content in rice was divided into four levels from low to high, according to the colors from brown red to dark blue after staining. The evaluation on the amylose content in 28 rice varieties by using the method showed that there was a significant correlation between the staining results and standard value (r = 0.95**). The method was applied to the improvement of rice amylose content in low generations, and the accuracy of the method in Wx gene screening was verified by molecular marker assistant selection. The amylose content evaluated by this method was highly coincident with rice starch synthesisrelated gene markers in 22 breeding parents for restorer and maintainer lines of hybrid rice.

Key words： rice; amylose content; quality improvement; low generation; staining method; molecular marker assistant selection; breeding

Cite this article

WANG Yue-xing,NI Shen,CHEN Hong-qi, LIU Gui-fu,YANG Jun, DUAN Bin-wu,ZHU Xu-dong . Improvement of Method for Evaluating Amylose Content in Rice at Low Generations of Breeding[J]. Chinese Journal OF Rice Science, 2010 , 24(1) : 93 -98 . DOI: 10.3969/j.issn.1001-7216.2010.01.16

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