Genetic Diversity and Pathogenicity Variation of Different Rhizoctonia solani Isolates in Rice from Zhejiang Province, China

CHEN  Tao, ZHANG  Zhen, CHAI  Rong-yao,WANG Jiao-yu,MAO Xue-qin,QIU  Hai-ping,DU Xin-fa,JIANG  Hua, WANG  Li-an,WANG Yan-li

doi:DOI: 10.3969/j.issn.1001-7216.2010.12

2010 , Vol. 24 >Issue 1: 67 - 72

DOI: https://doi.org/DOI: 10.3969/j.issn.1001-7216.2010.12

研究报告

Genetic Diversity and Pathogenicity Variation of Different Rhizoctonia solani Isolates in Rice from Zhejiang Province, China

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1 Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2College of Life Science, Hebei Normal University, Shijiazhuang 050016, China; *Corresponding author, E-mail: wangylaa@yahoo.com.cn; sungc01@
sina.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-01-10

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Abstract

Sixty Rhizoctonia solani strains were isolated from rice sheath blight samples collected from ricegrowing regions of Zhejiang Province, China. Hyphal fusion identification revealed that all the 60 strains belonged to anastomosis group AG1, as they all fused with the three intraspecific group of AG1 group(AG1ⅠA, AG1ⅠB and AG1ⅠC). Fiftyeight strains out of the 60 strains fused with the standard strain of AGBⅠ group. Pathogenicity identification analysis in vitro indicated significant difference in pathogenicity among the strains. TTZF1, the most pathogenetic strain, was significantly more pathogenetic than the other 52 strains. Further internal transcribed spacer(ITS) analysis indicated that phylogenetic tree coincided with the result of hyphal fusion identification, and was irrelevant with pathogenicity difference.

Key words： rice sheath blight; Rhizoctonia solani; anastomosis group; internal transcribed spacer; phylogenesis; pathogenicity

Cite this article

CHEN Tao, ZHANG Zhen, CHAI Rong-yao,WANG Jiao-yu,MAO Xue-qin,QIU Hai-ping,DU Xin-fa,JIANG Hua, WANG Li-an,WANG Yan-li .

Genetic Diversity and Pathogenicity Variation of Different Rhizoctonia solani Isolates in Rice from Zhejiang Province, China

[J]. Chinese Journal OF Rice Science, 2010 , 24(1) : 67 -72 . DOI: DOI: 10.3969/j.issn.1001-7216.2010.12

References

[1]檀根甲, 陈莉, 胡道平, 等. 水稻纹枯病菌菌核存活力及地下侵染的研究. 菌物系统, 2000, 19(1): 142-145.

[2]Anderson N A. The genetics and pathology of Rhizoctonia solani. Ann Rev Phytopathol, 1982, 20: 329-347.

[3]Schultz H. Vergleichende untersuchungen zurkologie, morphologie, and systematic des “vermehrungpilzes”. Arb Biol Reichanst Land Forstwirtsch, 1937, 22: 1-41.

[4]周而勋, 杨媚. 从植物病组织中分离立枯丝核菌的快速、简便技术. 华南农业大学学报, 1998, 19(1): 125-126.

[5]方中达. 植病研究方法. 北京: 农业出版社, 1979: 122-146.

[6]周而勋, 曹菊香, 杨媚, 等. 我国南方六省(区)水稻纹枯病菌遗传多样性的研究. 南京农业大学学报, 2002, 25(3): 36-40.

[7]Scott R P, Ziegler R S， Nelson R J. A procedure for miniscale preparation of Pyricularia grisea DNA. Intl Rice Res Notes, 1993, 18: 47-48.

[8]Gardes M, Bruns T D. ITS primer with enhanced specificity for basidiomycetes: Application to the identification of mycorrhizae and rusts. Mol Ecol Notes, 1993, 2(2): 113-118.

[9]White T J, Bruns T D, Lee S, et al. Analysis of phylogenetic relationships by amplification and direct sequencing of ribosomal RNA genes∥Innis M A. PCR Protocols: A Guide to Methods and Applications. New York: Academic Press, 1990: 15-22.

[10]周而勋, 杨媚. 广东省水稻纹枯病菌的致病力和融合群研究. 广东农业科学, 1999(5): 36-38.

[11]易润华, 梁承邺, 朱西儒, 等. 广东省水稻纹枯病菌遗传多样性与致病力分化的研究. 热带亚热带植物学报, 2002, 10(2): 161-170.

[12]杨根华, 董文汉, 李梅, 等. 云南省水稻纹枯病菌系研究. 菌物系统, 2002, 21(2): 274-279.

[13]肖勇, 刘明伟, 李刚, 等. 四川省水稻立枯丝核菌(Rhizoctonia solani)的遗传分化与致病力. 中国水稻科学, 2008, 22(1): 87-92.

[14]刘立, 葛起新. 华东地区立枯丝核菌融合群鉴定. 浙江农业大学学报, 1987, 13(3): 227-233.

[15]李祥, 侯明生, 胡汉桥. 湖北省水稻纹枯病菌菌丝融合群鉴定. 华中农业大学学报, 1993, 12(1): 14-17.

[16]黄江华, 杨媚, 周而勋, 等. 广州地区10种作物立枯丝核菌菌丝融合群测定. 仲恺农业技术学院学报, 2002, 15(1): 14-18.

[17]蒙姣荣, 张超冲, 李界秋, 等. 广西水稻纹枯病菌菌丝融合群鉴定初报. 中国农学通报, 2006, 22(6): 327-329.

[18]Rosewich U L, Pettway R E, McDonald B A, et al. High levels of gene flow and heterozygote excess characterize Rhizoctonia solani AG1IA (Thanatephorus cucumeris) from Texas. Fungal Genet Biol, 1999, 28: 148-159.

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