Chinese Journal OF Rice Science

Chinese Journal OF Rice Science >

2010 , Vol. 24 >Issue 1: 18 - 24

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.01.04

研究报告

QTL Analysis of the Anoxic Tolerance at the Seedling Stage in Rice

Expand

  • 1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Department of Agricultural Resource and Environment, Heilongjiang University, Harbin 150008, China; *Corresponding author, E-mail: delinhong@njau.edu.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2010-01-10

Fold

Abstract

Coleoptile lengths(CL) of seedlings at 7 days after germination under anoxic stress and normal conditions were investigated in two permanently segregated populations and their parents in rice (Oryza sativa L.). By using anoxic response index, a ratio of CL under anoxic stress to CL under normal conditions, as indicator of seedling anoxic tolerance (SAT), QTLs for SAT were detected. Two loci controlling SAT, designated as qSAT-2-R and qSAT-7-R, were detected in the RIL population (247 lines) derived from a cross between Xiushui 79(japonica cultivar) and C Bao(japonica restorer line). qSAT-2-R, explaining 8.7% of phenotypic variation, was tightly linked with the SSR marker RM525. qSAT-7-R, explaining 9.8% of phenotypic variation, was tightly linked with the marker RM418. The positive alleles of the two loci came from C Bao. Six loci controlling SAT, designated as qSAT-2-B, qSAT-3-B, qSAT-5-B, qSAT-8-B, qSAT-9-B and qSAT-12-B, were detected in the BIL population (98 lines) derived from a backcross of Nipponbare (japonica) /Kasalath (indica)//Nipponbare(japonica). The positive alleles of qSAT-2-B, qSAT-3-B and qSAT-9-B, which explained 16.2%, 11.4% and 9.5% of phenotypic variation, respectively, came from Nipponbare. The positive alleles qSAT-5-B, qSAT-8-B and qSAT-12-B, which explained 7.3%, 5.8% and 14.0% of phenotypic variation, respectively, were from Kasalath. The RFLP marker C747, linked tightly with qSAT-2-B, corresponded to the SSR marker RM1367 according to physical map. Genetic distance between qSAT-2-R and qSAT-2-B was 7.2 cM.

Key words： rice (Oryza sativa); seedling; anoxic tolerance; quantitative trait locus; recombinant inbred line; backcross recombinant inbred line

Cite this article

WANG Yang,GUO Yuan,HONG Delin . QTL Analysis of the Anoxic Tolerance at the Seedling Stage in Rice [J]. Chinese Journal OF Rice Science, 2010 , 24(1) : 18 -24 . DOI: 10.3969/j.issn.1001-7216.2010.01.04

References

[1]陈健. 水稻栽培方式的演变与发展研究. 沈阳农业大学学报, 2003, 34(5): 389-393.
[2]景德道, 刁立平, 钱华飞, 等. 水稻直播与移栽的比较及相应育种策略. 江西农业学报, 2008, 20(7): 17-20.
[3]吴文革, 陈烨, 钱银飞, 等. 水稻直播栽培的发展概况与研究进展. 中国农业科技导报, 2006, 8(4): 32-36.
[4]Fred T T, Chen C C, Garry N M. Morphological development of rice seedling in water at controlled oxygen levels. Agron J, 1981, 73: 556-560.
[5]Yamauchi M, Aguilar A M, Vaughan D A, et al. Rice ( Oryza sativa L.) germplasm suitable for direct sowing under flooded soil surface. Euphytica, 1993, 67: 177-184.
[6]Yamauchi M, Biswas J K. Rice cultivar difference in seedling establishment in flooded soil. Plant & Soil, 1997, 189: 145-153.
[7]Setter E S, Ella E S, Valdez A P. Relationship between coleoptile elongation and alcoholic fermentation in rice exposed to anoxia: Ⅱ. Cultivar differences. Ann Bot, 1994, 74: 273-279.
[8]侯名语, 江玲, 王春明, 等. 水稻种子低氧发芽力的 QTL定位和上位性分析. 中国水稻科学, 2004, 18(6): 483-488.
[9]王洋, 王盈盈, 洪德林. 太湖流域水稻种子活力和耐缺氧能力遗传变异研究. 南京农业大学学报, 2009, 32（3）： 1-7.
[10]郭媛, 程保山, 洪德林. 粳稻SSR连锁图谱的构建及恢复系卷叶性状QTL分析. 中国水稻科学, 2009, 23（3）: 256-262.
[11]Wang S C, Basten C J, Zeng Z B. Windows QTL Cartographer. Version 2.5[CP/OL]. [2006-09-15]. http://statgen.ncsu.edu/qtlcart/WinQTLCart.htm.
[12]McCouch S R, Cho Y G, Yano M, et al. Report on QTL nomenclature. Rice Genet Newsl, 1997, 14: 11-13.
[13]盖钧镒. 试验统计方法. 北京: 中国农业出版社, 2000: 35-125.
[14]McCouch S R, Teytelman L, Xu Y, et al. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res, 2002, 9: 199-207.
[15]Gibbs J, Morrell S, Valdez A, et al. Regulation of alcoholic fermentation in coleoptiles of two rice cultivars differing in tolerance to anoxia. J Exp Bot, 2000, 51: 785-796.
[16] LasanthiKudahettige R, Magneschi L, Loreti E, et al. Transcript profiling of the anoxic rice coleoptile. Plant Physiol, 2007, 144(1): 218-231.
Options
Outlines

/

〈 〉

Tel: 0571-63370278　E-mail: cjrs@263.net
Supported by Beijing Magtech Co., Ltd.