Germination Characteristics of Seeds under Salt Stress and Physiological Basis of Salt-Tolerance of Seedlings in Hybrid Rice
RUAN Song-lin; XUE Qing-zhong
(Department of Agronomy; College of Agriculture and Biotechnology; Zhejiang University; Hangzhou 310029; China)
Abstract Seeds of two hybrid rice combinations, Shanyou 10 and Liangyoupeijiu, were germinated in H 2O, 50 mmol/L, 100 mmol/L or 150 mmol/L NaCl solutions at 30℃, germination performance, activities of α,β-amylase and content of malondialdehyde(MDA) and compatible solutes, proline, total soluble sugar, fructose and sucrose were determined. Results showed that germination index were significantly reduced and mean germination time were prolonged significantly under salt stress. Except that activities of α-amylase in Shanyou 10 seeds were increased under 50 mmol/L NaCl concentration, activities of α,β-amylase were decreased under salt stress as compared with the control (H 2O). Activities of protective enzymes, superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT), and content of MDA and compatible solutes, proline, total soluble sugar, fructose and sucrose, differ at different concentrations of NaCl solutions, but their regular trends were not found. Relative water content and tolerance ratio of hybrid rice seedlings were decreased with gradient enhancement of NaCl concentrations. It also indicated that Shanyou 10 had better germination performance than Liangyoupeijiu. Activities of α,β-amylase, protective enzymes, SOD, POD and CAT, and content of MDA and compatible solutes, proline, total soluble sugar, fructose and sucrose, in Shanyou 10 were significantly greater than those in Liangyoupeijiu. It suggested that Shanyou 10 had better salt tolerance of seedlings than that of Liangyoupeijiu.
Key words： salt stress
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