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  2005, Vol. 19 Issue (2): 167-173     DOI:
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Frequency and Distribution of Microsatellites in Open Reading Frame of Rice Blast Fungus, Magnaporthe grisea
LI Cheng-yun , LI Jin-bin , ZHOU Xiao-Gang , ZHANG Shao-song , DONG Ai-rong , XU Ming-hui
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Abstract Abundance and distribution of microsatellite (simple sequence repeats, SSR) with repeated unit lengths of 1-6 base pairs in protein coding region of whole genome in rice blast fungus, Magnaporthe grisea was examined. The result showed that 3 240 SSRs (longer than 15 bp, 80% matches) were found within 2 387 genes. Among 4 732 annotated genes up to now, SSRs were found in 861 genes. Tri and hexa nucleotide repeats prevailed in protein coding exons of the genome, whereas, mono , di , tetra and penta nucleotide repeats were rare. The genes in which SSRs were most frequently found related to cellular regulation, and their position in genes varied among genes. SSRs were rarely conserved between genes with related sequences, suggesting high instability or a recent origin. The preferred location of SSR within the open reading frames of genes related to cellular regulation(transcription, signaling transduction) together with their instability suggested that SSR could have an important role in speciation. The instability of SSR distribution within protein coding regions suggested that analysis of polymorphism and functional role of these SSRs could benefit the functional genomics of M. grisea.
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LI Cheng-yun
LI Jin-bin
ZHOU Xiao-Gang
ZHANG Shao-song
DONG Ai-rong
XU Ming-hui
Key wordsMagnaporthe grisea   genome   simple sequence repeat   distribution   protein coding region   functional genomics     
Received: 1900-01-01;
Cite this article:   
LI Cheng-yun ,LI Jin-bin ,ZHOU Xiao-Gang et al. Frequency and Distribution of Microsatellites in Open Reading Frame of Rice Blast Fungus, Magnaporthe grisea [J]. , 2005, 19(2): 167-173 .
 
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