Chinese Journal OF Rice Science

Chinese Journal of Rice Science

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Breeding of Restorer Lines of Hybrid Rice with Bacterial Blight Resistance Gene Xa23 by Using MarkerAssisted Selection

ZHENG Jiatuan1, TU Shihang1， ZHANG Jianfu1， ZHENG Yi1， ZHAO Kaijun2, ZHANG Shuijin1, XIE Huaan1，*   

  1. 1Rice Research Institute/Key Laboratory of Crop Germplasm Utilization between Fujian and Taiwan, Ministry of Agriculture/Chinese National Center for Rice Improvement (Fuzhou Branch)/Fujian Engineering and Technology Research Center of Hybrid Rice Breeding/Fujian Engineering Laboratory of Crop Molecular Breeding, Fujian Academy of Agricultural Sciences, Fuzhou 350018, China; 2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081， China; *Corresponding author, E-mail: huaanxie@yahoo.com.cn
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-10 Published:2009-07-10

含白叶枯病抗性基因Xa23水稻恢复系的分子标记辅助选育

郑家团1,涂诗航1,张建福1,郑 轶1,赵开军2,张水金1,谢华安1，*   

  1. 1福建省农业科学院 水稻研究所/农业部闽台农作物种质资源利用重点开放实验室/福州国家水稻改良分中心/福建省杂交水稻育种工程技术研究中心/福建省作物分子育种工程实验室， 福建 福州 350018； 2中国农业科学院 作物科学研究所， 北京 100081； *通讯联系人，E-mail: huaanxie@yahoo.com.cn

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Abstract: The bacterial blight resistance gene Xa23 from a NIL CBB23 was introduced into a restorer line HK01，which is susceptible to bacterial blight, by crossing and markerassisted selection. Twelve homologous restorer lines with Xa23 were obtained through testing the flanking marker C189. An elite restorer line (K10) with high resistance to bacterial blight and good restoring ability and high yield potential was selected.

Key words: hybrid rice, bacterial blight, markerassisted selection, restorer line, resistance gene, breeding

摘要： 通过常规杂交育种技术和分子标记辅助选择技术，对携有白叶枯病抗性基因Xa23的亲本材料CBB23进行回交转育和农艺性状改良。通过分子标记辅助选择检测目的基因，快速获得了12份具有Xa23基因的恢复系稳定材料。采用白叶枯病菌株的专化强毒菌系P6对该12份材料进行室内接种鉴定，筛选出1份高抗白叶枯病的抗性新恢复系材料。应用3个不同的不育系对该恢复系进行测交，结果表明该恢复系具有良好的恢复性及产量潜力。

关键词: 杂交水稻, 白叶枯病, 分子标记辅助选择, 恢复系, 抗性基因, 育种