Abstract: A new cosmid vector pSVhygB containing hygromycin B resistance gene and bacterlophage λcos site was constructed. Using the pSVhygB the genomic DNA library of Magnaporthe grisea was constructed. It showed that pSVhygB can stably transform M. grisea with frequency 15 transformants per microgram DNA and electroporation could not improved the transformation efficiency. There approximately had 10,000 recombinant clones in our genomic DNA library, more than that of the requirement for M. grisea.

Key words: cosmid vector, genomic DNA library, hygromycin B resistant marker, Magnaporthe grisea, transformation

摘要： 用潮霉素B（ hygromycin B）抗性基因和λ噬菌体的cos位点组建了一种cosmid质粒pSVhygB，以此质粒建立了稻瘟病菌的转化体系，并且构建了病菌的基因组文库。结果表明，pSVhygB质粒可用于稳定地转化稻瘟病菌，转化频率约为15个转化子／μg DNA，电击法转化并不能有效地提高稻瘟病菌的转化频率。构建的稻瘟病基因文库中包含将近10000个重组子克隆，随机挑取12个重组子的鉴定表明，都包含有外源插入片段，说明所建文库已达要求。

关键词: cosmid质粒, 基因组文库, 潮霉素B抗性标记