中国水稻科学
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  1995, Vol. 9 Issue (3): 156-160     DOI:
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Establishment of Transformation System and Genomic DNA Library of Magnaporthe grisea with Cosmid Vector
Lu Guodong; Huang Danian; Tao Quanzhou; Yang Wei; Xie Lianhui
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Abstract A new cosmid vector pSVhygB containing hygromycin B resistance gene and bacterlophage λcos site was constructed. Using the pSVhygB the genomic DNA library of Magnaporthe grisea was constructed. It showed that pSVhygB can stably transform M. grisea with frequency 15 transformants per microgram DNA and electroporation could not improved the transformation efficiency. There approximately had 10,000 recombinant clones in our genomic DNA library, more than that of the requirement for M. grisea.
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Articles by authors
Lu Guodong
Huang Danian
Tao Quanzhou
Yang Wei
Xie Lianhui
Key wordscosmid vector   genomic DNA library   hygromycin B resistant marker   Magnaporthe grisea   transformation     
Received: 1900-01-01;
Cite this article:   
Lu Guodong,Huang Danian,Tao Quanzhou et al. Establishment of Transformation System and Genomic DNA Library of Magnaporthe grisea with Cosmid Vector[J]. , 1995, 9(3): 156-160 .
 
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