中国水稻科学
     Home | About Journal | Editorial Board | Publication Ethics Statement | Subscriptions | Advertisement | Contacts Us | Chinese
  2011, Vol. 25 Issue (3): 249-255     DOI: 10.3969/j.issn.1001-7216.2011.03.004
研究报告 Current Issue | Next Issue | Archive | Adv Search  |   
Analysis of Special DNA Methylated Sites Between Haploid of TwinSeedling and Its Hybrids in Rice
WU Shao-hua, XUE Jing-jing, ZHANG Hong-yu, XU Pei-zhou, WU Xian-jun*
Rice Research Institute/Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Chengdu 611130, China; *Corresponding author, E-mail: wuxjsau@126.com
 Download: PDF (1231 KB)   HTML (1 KB)   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract The DNA cytosine methylation mutation at the 5′CpCpGpG sites of the haploid of SARII628, and its hybrids with Shuhui 527, Shuhui 363 were analyzed by employing the MSAP (methylation sensitive amplification polyphisim) method. A total of 765 DNA methylated sites were detected and the methylation level of hybrids was lower than that of the parents. Meanwhile, different bands between hybrids and parents were analyzed and two types of methylated sites were detected, of which one inherited from the haploid, and the other didn′t. The biological functions of genes related to methylated sites involved in cell structure, metabolize and stress reaction. Therefore, DNA methylated modifications may be involved in the regulation of related genes, playing an important role in plant growth, development and envolution.
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
WU Shao-hua
XUE Jing-jing
ZHANG Hong-yu
XU Pei-zhou
WU Xian-jun*
Key wordstwin-seedling rice   haploid   methylation sensitive amplification polymorphism   DNA methylation     
Received: 1900-01-01;
Cite this article:   
WU Shao-hua,XUE Jing-jing,ZHANG Hong-yu et al. Analysis of Special DNA Methylated Sites Between Haploid of TwinSeedling and Its Hybrids in Rice[J]. , 2011, 25(3): 249-255 .
 
[1] 吴乃虎. 基因工程原理. 北京: 科学出版社, 2001: 86-88.
[2] Richards E J. DNA methylation and plant development. Trends Genet, 1997, 13: 319-323.
[3] Chan S W, Henderson I R, Jacobsen S E. Gardening the genome: DNA methylation in Arabidopsis thaliana. Nat Rev Genet, 2005, 6(5): 351-360.
[4] Bird A P, Wolffe A P. Methylation-induced repression: Belts, braces, and chromatin. Cell, 1999, 99(5): 451-454.
[5] Bestor T H. The DNA methyltransferases of mammals. Human Mol Genet, 2000, 9(16): 2395-2402.
[6] Bender J. DNA methylation and epigenetics. Annu Rev Plant Biol, 2004, 55: 41-68.
[7] Gehring M, Choi Y, Fischer R L. Imprinting and see development. Plant Cell, 2004, 16(Suppl): S203-S213.
[8] Wassenegger M. RNA-directed DNA methylation. Plant Mol Biol, 2002, 43: 203-220.
[9] Masterson J. Stomatal size in fossil plants evidence for polyploidy in majority of angiosperms. Science, 1994, 264: 421-424.
[10] Bowers J E, Chapman B A, Rong J, et al. Unravelling angiosperm genome evolution by phylogenetic analysis of chromosomal duplication events. Nature, 2003, 422: 433-438.
[11] Zhang H Y, Peng H, LI Y, et al. Patterns of DNA cytosine methylation between haploids and corresponding diploids in rice. Chin Sci Bull, 2006, 51(14): 1721-1728.
[12] Peng H, Zhang H Y, Li Y, et al. Natural homologous triploidization and DNA methylation in SARII-628, a twin-seedling rice (Oryza sativa L.). Rice Sci, 2007, 14(4): 265-271.
[13] Xiong L Z, Xu C G, Saghai Maroof M A, et al. Patterns of cytosine methylation in an elite rice hybrid and its parental lines detected by a methylation-sensitive amplification polymorphism technique. Mol Gen Genet, 1999, 261: 439-446.
[14] Mcclelland M, Nelson M, Raschke E. Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nucl Acids Res, 1994, 22(17): 3640-3659.
[15] Reyna-Lopez G E, Simpson J, Ruiz-Herrera J. Differences in DNA methylation patterns are detectable during the dimorphic transition of fungi by amplification of restriction polymorphism. Mol Gen Genet, 1997, 253(6): 703-710.
[16] Okuda K, Nakamura T, Sugita M, et al. A pentatricopeptide repeat protein is a site recognition factor in chloroplast RNA editing. JBC, 2006, 281(49): 37661-37667.
[17] Zehrmann A, Verbitskiy D, van der Merwe J A, et al. A DYW domain-containing pentatricopeptide repeat protein is required for RNA editing at multiple sites in mitochondria of Arabidopsis thaliana. Plant Cell, 2009, 21: 558-567.
[18] Yi Z B, Sun Y, Niu T T, et al. Patterns of DNA cytosine methylation between hybrids and their parents in sorghum genome. Acta Agron Sin, 2005, 31(9): 1138-1143.
[19] 朱新霞, 汪保华, 郭旺珍, 等. 中棉所12配制的2个杂交棉DNA甲基化遗传与传递. 作物学报, 2009, 35(12): 2150-2158.
[20] He G M, Zhu X P, Elling A A, et al. Global epigenetic and transcriptional trends among two rice subspecies and their reciprocal hybrids. Plant Cell, 2010, 22: 17-33.
[21] 吴先军, 汪旭东, 周开达, 等. 从农艺性状和分子标记鉴定一个水稻三倍体×二倍体 F2 群体的遗传稳定性. 植物学报, 1999, 41(10): 1067-1071.
[22] 李云, 张红宇, 汪秀志, 等. 水稻同源三倍体(149-B)×二倍体F2代稳定群体的验证及分析. 遗传学报, 2004, 31(6): 604-608.
[23] Feng S, Cokus S J, Zhang X, et al. Conservation and divergence of methylation patterning in plants and animals. Proc Natl Acad Sci USA, 2010, 107(19): 8689-8694.
[24] Zemach A, McDaniel I E, Silva P, et al. Genome-wide evolutionary analysis of eukaryotic DNA methylation. Science, 2010, 328(5980): 916-919.
[1] JIANG Hua ,#,ZHAO Jiang-hong ,#,GUO Long-biao ,JIANG Liang ,XUE Da-wei,ZENG Da-li,QIAN Qian,SUN Guo-chang . QTL Mapping and Epistatic Analysis of High-Order Tillering in Rice (Oryza sativa)[J]. , 2011, 25(2): 157-162 .
[2] XU Qun,YUAN Xiao-ping,YU Han-yong,WANG Yi-ping,TANG Sheng-xiang,WEI Xing-hua* . Mapping QTLs for Drought Resistance at Seedling Stage in Rice by Using a Doubled Haploid Population[J]. , 2010, 24(5): 469-473 .
[3] HU Jiang ,Kan FUJIMOTO ,GUO Long-biao ,ZENG Da-li ,ZHANG Guang-heng ,DONG Guo-jun ,WANG Xiao-hu ,ZHU Li-huang ,QIAN Qian. QTL Analysis of Lodging Resistance Force and Lodging Resistance-Related Traits in Rice[J]. , 2008, 22(2): 211-214 .
[4] JIANG Hua ,JIANG Liang ,ZHAO Jiang-hong ,GUO Long-biao ,SUN Huan-ming ,XUE Da-wei ,ZENG Long-jun ,ZENG Da-li ,LIANG Guo-hua ,QIAN Qian. QTL Mapping of Tolerance to Photooxidation in Rice[J]. , 2008, 22(1): 38-44 .
[5] PENG Mao-min ,YANG Guo-hua ,ZHANG Jing-jing ,AN Bao-guang ,LI Yang-sheng. QTL Analysis for Flag Leaf Morphological Traits in Rice(Oryza sativa L.) Under Different Genetic Backgrounds[J]. , 2007, 21(3): 247-252 .
[6] YANG Chang-deng ,GUO Long-biao ,LI Xi-ming ,JI Zhi-juan ,MA Liang-yong ,QIAN Qian. Analysis of QTLs for Resistance to Rice Bakanae Disease[J]. , 2006, 20(6): 657-659 .
[7] YANG Chang-deng ,ZENG Da-li ,MA Liang-yong ,JI Zhi-juan ,GUO Long-biao ,LI Xi-ming ,QIAN Qian. Mapping QTLs for Bacterial Blight Resistance in a DH Population from japonica/indica Cross of Rice (Oryza sativa)[J]. , 2006, 20(1): 102-104 .
[8] ZHANG Guang-heng ,ZHANG Guo-ping ,QIAN Qian ,XU Lu-ping ,ZENG Da-li ,TENG Sheng ,BAO Jin-song . QTL Analysis of Grain Shape Traits in Different Environments[J]. , 2004, 18(1): 16-22 .
[9] SOGAWA Kazushige ,TENG Sheng ,QIAN Qian ,ZENG Da-li ,ZENG Juan ,QIAN Zhong-hai ,ZHU Li-huang . Detection of QTLs Affecting Whitebacked Planthopper Resistance in an Indica/Japonica Doubled Haploid Rice Population[J]. , 2003, 17(ZK): 77-83 .
[10] YU Yan-chun ,TENG Sheng ,ZENG Da-li ,DONG Guo-jun ,QIAN Qian ,HUANG Da-nian ,ZHU Li-huang . Analysis of QTLs for Resistance to Rice Bacterial Blight[J]. , 2003, 17(4): 315-318 .
[11] DONG Guo-jun ,Hiroshi FUJIMOTO ,TENG Sheng ,HU Xing-ming ,ZENG Da-li ,GUO Long-biao ,QIAN Qian . QTL Analysis of Flag Leaf Angle in Rice[J]. , 2003, 17(3): 219-222 .
[12] CAO Li-yong ,ZHU Jun ,YAN Qi-chuan ,HE Li-bin ,WEI Xing-hua ,CHENG Shi-hua . Mapping QTLs with Epistasis for Mesocotyl Length in a DH Population from indica-japonica Cross of Rice(Oryza sativa)[J]. , 2002, 16(3): 221-224 .
[13] ZENG Da-li ,QIAN Qian ,RUAN Liu-qing ,TENG Sheng ,Kunihiro YASUFUMI ,Fujimoto HIROSHI ,ZHU Li-huang . QTL Analysis of Chalkiness Size in Three Dimensions[J]. , 2002, 16(1): 11-14 .
[14] CHENG Shi-hua,MAO Chuan-zao,ZHAN Xiao-deng,SI Hua-min,SUN Zong-xiu. Construction of Double Haploid (DH) and Recombinant Inbred Line (RIL) Population of Indica-Japonica Hybrid and Their Differential in Indica and Japonica Property[J]. , 2001, 15(4): 257-260 .
[15] Yan Changjie,Li Xin,Cheng Zhukuan,Yu Hengxiu,Gu Minghong,Zhu Lihuang. Identification of QTL for Cold Tolerance at Early Seedling Stage in Rice(Oryza sativa) via RFLP Markers[J]. , 1999, 13(3): 134-138 .
[16] Yang Changdeng,Wu Lianbin,Zhao Chengzhang. In vitro Regulation of Haploid Somaclonal Micro-Buds in Indica Rice[J]. , 1998, 12(4): 219-222 .
[17] Yang Changdeng,Tang Kexuan,Wu Lianbin,Li Yao,Zhao Chengzhang,Liu Guangjie,Shen Daleng. Transformation of Haploid Indica Rice Shoots with Snowdrop Lectin Gene(GNA) by Agrobacterium-Mediated[J]. , 1998, 12(3): 129-133 .
Copyright © Chinese Journal of Rice Science 浙ICP备05004719号-5
Supported by: Beijing Magtech