Development and Application of Functional Markers for Polyphenol Oxidase (PPO) Alleles in Rice
YANG Jie1; CAO Qing2; WANG Jun1; FAN Fang-jun1;ZHANG Yu-qiong2; ZHONG Wei-gong1,*
1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2College of Life Science, Anhui Agricultural University, Hefei 230036, China；*Corresponding author, E-mail:<A href=
Abstract The phenol reaction (PHR) phenotype is one of the criteria of rice classification and the gene controlling PHR has been identified. The positive PHR phenotype is conditioned by functional polyphenol oxidase(PPO) gene, and negative PHR phenotype is conditioned by nonfunctional PPO gene. To seek the markers for rice classification, two InDel markers, FMppo18 and FMppo29, were developed flanking on the 18 bp or 29 bp deletion in exon 3 of PPO alleles. Fortyfive rice materials were genotyped with the two markers and their PHR phenotypes were also investigated. Three accessions of wild rice and six indica rice varieties carried nondeletion functional PPO alleles. Among the 11 japonica rice materials, nine carried 18 bp deletion allele, and two carried 29 bp deletion alleles. However, the indica rice variety Dular carried 29 bp deletion allele and Longqing carried 18 bp deletion allele. Among the 23 weedy rice accessions, three accessions of Ludao collected from Lianyungang of Jiangsu Province, China, and four accessions of Tangdao collected from Huaiyuan, Laian, Quanjiao, and Feidong of Anhui Province, China in 1950s carried nondeletion PPO alleles. Among the 16 weedy rice accessions collected in recent years, 14 carried normal PPO alleles, and two carried 18 bp deletion PPO allele which was consistent with the cultivated rice in Jiangsu Province. These results showed that the positive PHR materials carried normal PPO alleles, and the negative PHR materials carried deletion PPO alleles, indicating that the phenotype perfectly matched with the genotype. Therefore, the two markers can be used in germplasm screening, markerassisted selection and evolution research in rice.
YANG Jie,CAO Qing,WANG Jun et al. Development and Application of Functional Markers for Polyphenol Oxidase (PPO) Alleles in Rice [J]. , 2011, 25(1): 37-42 .
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