Chinese Journal of Rice Science

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Genetic Analysis and Mapping of a ThermoSensitive White Stripe Leaf Mutant at the Seedling Stage in Rice(Oryza sativa)

LI Chao1, LIN Dong-zhi1,*, DONG Yan-jun1, *, YE Sheng-hai2, ZHANG Xiao-ming2   

  1. 1Laboratory of Plant Genetics and Functional Genes, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China; 2Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; *Corresponding author, E-mail: dzlin@shnu.edu.cn; dong@shnu.edu.cn
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-10 Published:2010-05-10

一个水稻苗期温敏感白色条斑叶突变体的遗传分析及基因定位

李超1,林冬枝1, * ,董彦君1, *,叶胜海2,张小明2   

  1. 1上海师范大学 生命与环境科学学院 植物遗传与功能基因研究室, 上海 200234; 2浙江省农业科学院 作物与核技术利用研究所, 浙江 杭州 310021; *通讯联系人, E-mail: dzlin@shnu.edu.cn; dong@shnu.edu.cn

Abstract: A thermosensitive white stripeleaf mutant (tws) was selected from the M2 progeny of a japonica rice variety, Jiahua 1, treated with 60Co γradiation. In comparison with the wild type parent (Jiahua 1), the mutant displayed a phenotype of white stripe on the 3rd and 4th leaves, but began to turn a normal green phenotype on the 5th leaf when grown at low temperatures (20℃, 24℃). And also the content of total chlorophyll showed an obvious decrease in the leaves with white stripe. These results suggest that the expression of the mutant trait was thermosensitive and correlated with the leaf age of seedlings. The genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene, designated as tws (thermosensitive white stripeleaf). In addition, by using SSR markers and an F2 segregating population derived from the cross between the tws mutant and 9311, the tws gene was mapped between MM3907 and MM3928 with a physical distance of 86 kb on chromosome 4.

Key words: rice(Oryza sativa), thermosensitivity, white stripeleaf, gene mapping

摘要: 通过对粳稻品种嘉花1号60Co γ射线诱变,从M2中筛选出一株低温敏感型白色条斑叶突变体(tws)。它在低温(20℃,24℃)条件下培养时,苗期第3和第4叶表现出白色条斑,而第5叶开始转为正常。低温条件下该突变体白斑叶片叶绿素含量明显下降。该突变体白色条斑叶性状具有温敏感性,且与叶龄相关。遗传分析表明,该突变性状受1对隐性核基因控制,定名为tws(thermosensitive white stripeleaf)基因。以tws突变体与籼稻9311杂交的F2分离群体作为定位群体,利用SSR标记将该基因定位在第4染色体MM3907和MM3928之间,其物理距离约为86 kb。

关键词: 水稻, 温度敏感性, 白色条斑叶