Chinese Journal of Rice Science

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Identification of QTL for Salt Tolerance in Rice Via Molecular Markers (in English)

Lin Hongxuan; Yanagihara S; Zhuang Jieyun; Senboku T; Zheng Kangle; Yashima S   

  • Received:1900-01-01 Revised:1900-01-01 Online:1998-04-10 Published:1998-04-10

应用分子标记检测水稻耐盐性的QTL

林鸿宣 1;柳原城司 2;庄杰云 1;仙北俊弘 2;郑康乐 1;八岛茂夫 2   

  1. 1 中国水稻研究所,中国杭州 310006 2 国际农林水产业研究中心冲绳亚热带站,日本,冲绳,石垣907

Abstract: The recombinant inbred (RI) lines (F7) were developed by single seed descent procedure from acombination, Tesanai 2/CB. The RI population was used to map QTLs controlling salt tolerance in rice. The 142 lines were genotyped at 60 RFLP markers and evaluated for seedling survival days (SD) in culture solution with EC= 12 dS/m by adding NaCl. Transgressive segregations were observed for salt tolerance in the RI population. Alinkage map consisting of 52 marker loci from 11 linkage groups was constructed. Of 60 marker loci, only one marker locus RG13 on chromosome 5, which associated significantly with salt tolerance was detected and this locus explained 11. 6 % of the observed phenotypic variance for SD. The allele from Tesanai 2 increased salt tolerance. Among 59 pairs of loci between RG13 and all other 59 marker loci, significant epistatic interactions were detected in three pairs which were RG13 and RG104 on chromosome 3 (P= 0. 0004), RG13 and RG143 on chromosome 4 (P= 0. 0028) , RG13 and RG716 on chromosome 6 (P = 0. 0063). The interactions between RG13 allele from Tesanai 2 and RG104 or RG143 from CB significantly increased salt tolerance level. The interaction between RG13 and RG716 alleies from Tesanai 2 also increased tolerance level. These gene interactions provided clear genetic evidence of transgression.

Key words: epistatic interaction, molecular marker, quantitative trait loci, salt tolerance

摘要: 利用特三矮2号/CB组合构建了重组自交系群体(RI)。以60个RFLP标记检测142个纯系的基因型。在含有NaCl的电导率为12 dS/m的培养液中鉴定这些纯系的耐盐性。结果表明,RI群体的耐盐性出现超亲分离。构建了一张覆盖11条染色体、含52个标记位点的连锁图。仅检测到一个位于第5染色体的位点(RG13)显著与耐盐性有连锁。该位点的表型贡献率为11.6%。来自母本的该位点可提高耐盐性。分别对RG13与其它59个标记位点间的互作做检测,仅发现3对互作显著,即RG13×RG104; RG13×RG143; RG13×RG716。当来自母本的RG13分别与来自父本的RG104和RG143重组时,均明显提高耐盐性。分别来自母本的RG13和RG716能产生提高耐盐性的互作。这些基因互作结果为超亲分离提供了理论依据。

关键词: 分子标记, 耐盐性, 数量性状位点, 上位互作