Abstract:

【Objective】This study aims to investigate the effects of nano-priming technology on rice seed germination under salt stress, in order to provide technical support for rice cultivation in saline-alkali soils.【Method】The japonica rice variety, Ningjing 47, was used as the experimental material. Salt stress treatments (0 and 150 mmol/L NaCl) were applied, and five concentrations of Fe-Mg nanoparticles (0, 50, 100, 200, and 400 mg/L) were tested under 150 mmol/L NaCl stress. The effects on seed germination, seedling growth, and physiological characteristics were examined.【Result】The results showed that salt stress significantly inhibited rice seed germination and seedling growth. Nano-priming did not significantly affect germination percentage or germination potential but enhanced seedling growth. It increased the activities of superoxide dismutase (by 27.78%-76.39%), peroxidase (by 14.05%-38.71%), and catalase (by 18.67%-120.00%), as well as the content of ascorbic acid (by 4.40%-225.79%), soluble sugars (by 63.08%-145.96%), proline (by 10.33%-16.88%), and chlorophyll (by 45.38%-105.31%). It also reduced malondialdehyde content (by 16.94%-32.41%) and enhanced the selective transport coefficient for K⁺ over Na⁺.【Conclusion】The concentration of 200 mg/L Fe-Mg nanoparticles was identified as optimal for alleviating salt stress and promoting rice germination and seedling growth.

Key words: rice, Fe-Mg nano-particle, seed priming, salt stress

摘要：

【目的】探究纳米引发技术对盐胁迫下水稻种子萌发的影响，为盐碱地水稻种植提供技术参考。【方法】以宁粳47号水稻为供试材料，设置盐胁迫处理(0、150 mmol/L NaCl)，并在150 mmol/L NaCl胁迫下，设置0、50、100、200、400 mg/L 5种浓度的Fe-Mg纳米颗粒处理，研究纳米引发对盐胁迫下水稻种子萌发、幼苗生长及生理特性的影响。【结果】盐胁迫显著抑制了水稻种子萌发和幼苗生长。纳米引发对水稻种子的发芽率和发芽势没有显著影响，但能促进幼苗生长，显著提高超氧化物歧化酶(27.78%~76.39%)、过氧化物酶(14.05%~38.71%)和过氧化氢酶(18.67%~120.00%)活性及维生素C(4.40%~225.79%)、可溶性糖(63.08%~145.96%)、脯氨酸(10.33%~16.88%)、叶绿素(45.38%~105.31%)含量，降低丙二醛含量(16.94%~32.41%)，提高钾离子选择交换系数。【结论】200 mg/L Fe-Mg纳米颗粒引发是缓解盐胁迫，促进水稻萌发和幼苗生长的最适浓度。

关键词: 水稻, Fe-Mg纳米颗粒, 种子引发, 盐胁迫