Synergistic Impact of Piriformospora indica and Compound Fertilizer on Rice Seedling Quality for Mechanical Transplanting

doi:10.16819/j.1001-7216.2025.240706

Abstract

Abstract:

【Objective】To explore the application method and effects of Piriformospora indica in rice production.【Method】Indica lines Taiyou 203 and ZY246 were employed in this study, with a standard dosage of 1 g/L P. indica solution applied. Additionally, grey relational analysis methods were utilized to comprehensively evaluate the impact of three treatments—P. indica, compound fertilizer, and their combination—on the quality of rice seedlings for mechanical transplanting.【Result】The combined application of P. indica and compound fertilizer significantly enhanced seedling growth, increased seedling biomass, chlorophyll content, root system activity, and nitrate reductase activity. Furthermore, it promoted the expression of growth-related genes.【Conclusion】These findings deepen the understanding of the mechanisms by which P. indica promotes rice growth and provide a theoretical basis and practical guidance for the application of P. indica in rice production, particularly during the seedling nursery phase for mechanical transplanting.

Key words: Piriformospora indica, rice mechanical transplanting, seedling quality, grey correlation analysis

摘要：

【目的】探索印度梨形孢(Piriformospora indica)在水稻生产中的使用方式及应用效果。【方法】以泰优203和ZY246籼稻品种为研究材料，以1 g/L的印度梨形孢菌液为基准施用量，运用灰色关联度分析方法，综合评估了印度梨形孢、复合肥及其组合施用三种处理方式对水稻机插秧秧苗素质的影响。【结果】复合肥不会影响印度梨形孢在水稻根部的正常定殖，可以将印度梨形孢与复合肥组合施用；两者的组合施用不仅能显著促进秧苗生长，提高秧苗生物量、叶绿素含量、根系活力和硝酸还原酶活性，还能促进生长相关基因的表达。【结论】本研究不仅深化了对印度梨形孢促进水稻生长机制的理解，而且为印度梨形孢在水稻生产中的实际应用，尤其是机插秧育秧环节，提供了理论依据和实践指导。

关键词: 印度梨形孢, 水稻机插秧育秧, 秧苗素质, 灰色关联度分析

HE Yong, ZHANG Shiqian, WANG Zhicheng, ZHAN Xiaokang, DING Yike, LIU Xiaorui, MA Susu, TIAN Zhihong. Synergistic Impact of Piriformospora indica and Compound Fertilizer on Rice Seedling Quality for Mechanical Transplanting[J]. Chinese Journal OF Rice Science, 2025, 39(3): 412-422.

何勇, 张诗骞, 王志成, 詹逍康, 丁一可, 刘晓瑞, 马素素, 田志宏. 印度梨形孢与复合肥组合施用对水稻机插秧秧苗素质的影响[J]. 中国水稻科学, 2025, 39(3): 412-422.

Figures/Tables 9

References 34

[1]	宋红梅, 李廷亮, 刘洋, 黄璐, 杨立帆. 我国近20年主要粮食作物产量、进出口及化肥投入变化特征[J]. 水土保持学报, 2023, 37(1): 332-339.
	Song H M, Li T L, Liu Y, Huang L, Yang L F. Temporal variation of main grain crops yield, import and export and fertilizer consumption of china in the past 20 years[J]. Journal of Soil and Water Conservation, 2023, 37(1): 332-339. (in Chinese with English abstract)
[2]	刘钦普, 濮励杰. 中国粮食主产区化肥施用时空特征及生态经济合理性分析[J]. 农业工程学报, 2019, 35(23): 142-150.
	Liu Q P, Pu L J. Spatiotemporal variation of fertilizer utilization and its eco-economic rationality in major grain production areas of China[J]. Transactions of the Chinese Society of Agricultural Engineering, 2019, 35(23): 142-150. (in Chinese with English abstract)
[3]	赵雪雁, 刘江华, 王蓉, 王晓琪. 基于市域尺度的中国化肥施用与粮食产量的时空耦合关系[J]. 自然资源学报, 2019, 34(7): 1471-1482.
	Zhao X Y, Liu J H, Wang R, Wang X Q. Spatial-temporal coupling relationship between chemical fertilizer application and grain yield in China at city scale[J]. Journal of Natural Resources, 2019, 34(7): 1471-1482. (in Chinese with English abstract)
[4]	贺飞, 吕晓. 山东省化肥施用量与粮食产量时空耦合格局研究[J]. 地理与地理信息科学, 2023, 39(4): 96-102.
	He F, Lü X. Spatial-temporal coupling pattern of fertilizer application rate and grain yield in Shandong Province[J]. Geography and Geo-Information Science, 2023, 39(4): 96-102. (in Chinese with English abstract)
[5]	杨玉苹, 孙炜琳, 朱立志. 农户生物菌肥购买意愿及行为的影响因素研究——基于山东省设施菜农的调研数据[J]. 中国农业资源与区划, 2019, 40(2): 49-55, 78.
	Yang Y P, Sun W L, Zhu L Z. The influence factors of farmer's purchasing intension and behavior of biological fertilizer in Shandong[J]. Chinese Journal of Agricultural Resources and Regional Planning, 2019, 40(2): 49-55, 78. (in Chinese with English abstract)
[6]	肖占文, 赵致禧, 赵芸晨, 程红玉, 马银山, 濮超, 付余业. 化肥减量下有机肥配施土壤调理剂和生物菌肥对玉米连作土壤的生态修复效应[J]. 中国土壤与肥料, 2023, 318(10): 48-54.
	Xiao Z W, Zhao Z X, Zhao Y C, Cheng H Y, Ma Y S, Pu C, Fu Y Y. The ecological restoration effect of organic fertilizer combined with soil conditioner and biological fertilizer on continuous cropping soil of maize seed production under chemical fertilizer reduction[J]. Soil and Fertilizer Sciences in China, 2023, 318(10): 48-54. (in Chinese with English abstract)
[7]	Sharma I, Raina A, Choudhary M, Apra, Kaul S, Dhar M K. Fungal endophyte bioinoculants as a green alternative towards sustainable agriculture[J]. Heliyon, 2023, 9(9): e19487.
[8]	Chitnis V R, Suryanarayanan T S, Nataraja K N, Prasad S R, Oelmüller R, Shaanker R U. Fungal endophyte-mediated crop improvement: The way ahead[J]. Frontiers in Plant Science, 2020, 11: 561007.
[9]	Solanki S, Gupta S, Kapoor R, Varma A. Chemically synthesized AgNPs and Piriformospora indica synergistically augment nutritional quality in black rice[J]. Journal of Fungi, 2023, 9(6): 611.
[10]	Murphy B R, Doohan F M, Hodkinson T R. Yield increase induced by the fungal root endophyte Piriformospora indica in barley grown at low temperature is nutrient limited[J]. Symbiosis, 2014, 62(1): 29-39.
[11]	Su Z Z, Wang T, Shrivastava N, Chen Y Y, Liu X X, Sun C, Yin Y F, Gao Q K, Lou B G. Piriformospora indica promotes growth, seed yield and quality of Brassica napus L.[J]. Microbiological Research, 2017, 199: 29-39.
[12]	Ansari M W, Trivedi D K, Sahoo R K, Gill S S, Tuteja N. A critical review on fungi mediated plant responses with special emphasis to Piriformospora indica on improved production and protection of crops[J]. Plant Physiology and Biochemistry, 2013, 70: 403-410.
[13]	Li Q, Kuo Y W, Lin K H, Huang W Q, Deng C S, Ye K W, Chen S P. Piriformospora indica colonization increases the growth, development, and herbivory resistance of sweet potato (Ipomoea batatas L.)[J]. Plant Cell Reports, 2021, 40(2): 339-350.
[14]	韦巧, 武美燕, 张文英, 徐乐, 陈建芳, 潘锐, 田小海. 内生真菌印度梨形孢对旱稻苗期生长及抗旱性的影响[J]. 生态学杂志, 2018, 37(9): 2642-2648.
	Wei Q, Wu M Y, Zhang W Y, Xu L, Chen J F, Pan R, Tian X H. Effect of the endophytic fungus Piriformospora indica on the growth and drought tolerance of rice seedling under drought stress[J]. Chinese Journal of Ecology, 2018, 37(9): 2642-2648. (in Chinese with English abstract)
[15]	潘锐, 邓晶, 胡爱兵, 张文英. 印度梨形孢促进棉花幼苗生长及诱导提高苗期抗旱性研究[J]. 干旱地区农业研究, 2019, 37(5): 249-256, 261.
	Pan R, Deng J, Hu A B, Zhang W Y. Growth promotion and tolerance improvement to drought stress of cotton seedling conferred by Piriformospora indica[J]. Agricultural Research in the Arid Areas, 2019, 37(5): 249-256, 261. (in Chinese with English abstract)
[16]	高洁, 田培聪, 党悦嘉, 李昕芮, 王趁, 刘闯, 黄玉茜. 印度梨形孢-花生共生体系建立及对花生的促生作用[J]. 中国油料作物学报, 2024, 46(2): 385-393.
	Gao J, Tian P C, Dang Y J, Li X R, Wang C, Liu C, Huang Y Q. Establishment of Piriformospora indica-peanut symbiosis system and its promoting effect on peanut growth[J]. Chinese Journal of Oil Crop Sciences, 2024, 46(2): 385-393. (in Chinese with English abstract)
[17]	夏杨, 李传明, 刘琴, 韩光杰, 徐彬, 黄立鑫, 祁建杭, 陆玉荣, 徐健. 印度梨形孢对盐胁迫下水稻幼苗生长及抗氧化系统的影响[J]. 中国水稻科学, 2023, 37(5): 543-552.
	Xia Y, Li C M, Liu Q, Han G J, Xu B, Huang L X, Qi J H, Lu Y R, Xu J. Effects of Piriformospora indica on the growth and antioxidant system of rice seedlings under salt stress[J]. Chinese Journal of Rice Science, 2023, 37(5): 543-552. (in Chinese with English abstract)
[18]	舒珊, 高中南, 袁听, 陈强, 朱志炎, 何勇, 叶开温, 田志宏. 印度梨形孢最适培养基的筛选及其对水稻的促生作用研究[J]. 福建农业学报, 2019, 34(2): 155-161.
	Shu S, Gao Z N, Yuan T, Chen Q, Zhu Z Y, He Y, Ye K W, Tian Z H. Optimized culture medium and effect of Piriformospora indica on growth of rice plants[J]. Fujian Journal of Agricultural Sciences, 2019, 34(2): 155-161. (in Chinese with English abstract)
[19]	刘雪琳, 朱志炎, 何勇, 叶开温, 田志宏. 内生真菌印度梨形孢对水稻苗期耐盐性的影响[J]. 南方农业学报, 2019, 50(4): 719-725.
	Liu X L, Zhu Z Y, He Y, Ye K W, Tian Z H. Effects of endophytic fungus Piriformospora indica on salt stress tolerance of rice seedling[J]. Journal of Southern Agriculture, 2019, 50(4): 719-725. (in Chinese with English abstract)
[20]	吴金丹, 陈乾, 刘晓曦, 林福呈, 高其康, 楼兵干. 印度梨形孢对水稻的促生作用及其机理的初探[J]. 中国水稻科学, 2015, 29(2): 200-207.
	Wu J D, Chen Q, Liu X X, Lin F C, Gao Q K, Lou B G. Preliminary study on mechanisms of growth promotion in rice colonized by Piriformospora indica[J]. Chinese Journal of Rice Science, 2015, 29(2): 200-207. (in Chinese with English abstract)
[21]	刘繁超, 方淑梅, 王庆燕, 王晗昕, 牛娟娟, 梁喜龙. 不同浓度外源氨基酸对水稻秧苗生长及相关生理指标的影响[J]. 作物杂志, 2024, 219(2): 71-79.
	Liu F C, Fang S M, Wang Q Y, Wang H X, Niu J J, Liang X L. Effects of different concentrations of exogenous amino acids on growth and related physiological indicators of rice seedlings[J]. Crops, 2024, 219(2): 71-79. (in Chinese with English abstract)
[22]	麦迎晓, 黄慧灵, 程思忍, 孙晓銮, 唐湘如. 不同拌种处理对超级稻机插秧苗素质的影响[J]. 中国稻米, 2018, 24(3): 71-75.
	Mai Y X, Huang H L, Cheng S R, Sun X L, Tang X R. Effects of different seed dressing treatments on seedling quality of mechanical transplanting super rice[J]. China Rice, 2018, 24(3): 71-75. (in Chinese with English abstract)
[23]	中华人民共和国农业部.水稻工厂化育秧技术要求: NY/T 1534—2007[S]. 北京: 中国农业出版社, 2008.
	Ministry of Agriculture of the People's Republic of China.Technical requirement for rice factory seedling nursing: NY/T 1534—2007[S]. Beijing: China Agriculture Press, 2008. (in Chinese)
[24]	于林惠, 丁艳锋, 薛艳凤, 凌启鸿, 袁钊和. 水稻机插秧田间育秧秧苗素质影响因素研究[J]. 农业工程学报, 2006, 22(3): 73-78.
	Yu L H, Ding Y F, Xue Y F, Ling Q H, Yuan Z H. Factors affacting rice seedling quality of mechanical transplanting rice[J]. Transactions of the Chinese Society of Agricultural Engineering, 2006, 22(3): 73-78. (in Chinese with English abstract)
[25]	唐志强, 王昌华, 蒋洪波, 姚继攀, 刘军, 董立强, 马亮, 李跃东. 营养配比对壤土育秧秧苗素质及营养吸收的影响[J]. 西南农业学报, 2018, 31(8): 1662-1668.
	Tang Z Q, Wang C H, Jiang H B, Yao J P, Liu J, Dong L Q, Ma L, Li Y D. Effects of nutrient ratio on quality and nutrient uptake of loam seedling[J]. Southwest China Journal of Agricultural Sciences, 2018, 31(8): 1662-1668. (in Chinese with English abstract)
[26]	张慧敏, 耿若飞, 王二伟, 常鸿杰, 贾真真, 王春英, 樊振杰, 晋海金. 灰色关联度分析和DTOPSIS法在综合评价大豆新品种(系)中的应用[J]. 大豆科技, 2022, 177(2): 14-21.
	Zhang H M, Geng R F, Wang E W, Chang H J, Jia Z Z, Wang C Y, Fan Z J, Jin H J. Application of grey correlation degree analysis and DTOPSIS methods in comprehensive evaluation of new soybean varieties (lines)[J]. Soybean Science & Technology, 2022, 177(2): 14-21. (in Chinese with English abstract)
[27]	张兰, 宋谦, 马淑梅, 余小亮, 王纯喜, 杨晓. 利用灰色关联度法评价12个苜蓿品种的营养价值[J]. 中国饲料, 2023, 725(9): 136-140, 161.
	Zhang L, Song Q, Ma S M, Yu X L, Wang C X, Yang X. Applying grey correlation degree analysis to evaluate nutritional value of 12 Medicago sativa varieties[J]. China Feed, 2023, 725(9): 136-140, 161. (in Chinese with English abstract)
[28]	王淑君, 解慧芳, 邢璐, 宋慧, 宋中强, 张扬, 闫宏山, 刘海萍, 李龙, 王素英, 刘金荣. 基于主成分分析和灰色关联度分析的谷子品种(系)综合评价[J]. 江苏农业科学, 2023, 51(15): 42-49.
	Wang S J, Xie H F, Xing L, Song H, Song Z Q, Zhang Y, Yan H S, Liu H P, Li L, Wang S Y, Liu J R. Comprehensive evaluation of millet varieties (lines) based on principal component analysis and grey relational analysis[J]. Jiangsu Agricultural Sciences, 2023, 51(15): 42-49. (in Chinese with English abstract)
[29]	Bandyopadhyay P, Yadav B G, Kumar S G, Kumar R, Kogel K H, Kumar S. Piriformospora indica and Azotobacter chroococcum consortium facilitates higher acquisition of N, P with improved carbon allocation and enhanced plant growth in Oryza sativa[J]. Journal of Fungi, 2022, 8(5): 453.
[30]	Scholz S S, Barth E, Clément G, Marmagne A, Ludwig-Müller J, Sakakibara H, Kiba T, Vicente-Carbajosa J, Pollmann S, Krapp A, Oelmüller R. The root-colonizing endophyte Piriformospora indica supports nitrogen-starved Arabidopsis thaliana seedlings with nitrogen metabolites[J]. International Journal of Molecular Sciences, 2023, 24(20): 15372.
[31]	Ngwene B, Boukail S, Söllner L, Franken P, Andrade-Linares D R. Phosphate utilization by the fungal root endophyte Piriformospora indica[J]. Plant and Soil, 2016, 405(1-2): 231-241.
[32]	Yu C Y, Liu Y H, Zhang A D, Su S, Yan A, Huang L L, Ali I, Liu Y, Forde B G, Gan Y B. MADS-box transcription factor OsMADS25 regulates root development through affection of nitrate accumulation in rice[J]. PLoS One, 2015, 10(8): e0135196.
[33]	Fang Z M, Xia K F, Yang X, Grotemeyer M S, Meier S, Rentsch D, Xu X L, Zhang M Y. Altered expression of the PTR/NRT1 homologue OsPTR9 affects nitrogen utilization efficiency, growth and grain yield in rice[J]. Plant Biotechnology Journal, 2013, 11(4): 446-458.
[34]	Liu H C, Senthilkumar R, Ma G Y, Zou Q C, Zhu K Y, Shen X L, Tian D Q, Hua M S, Oelmüller R, Yeh K W. Piriformospora indica-induced phytohormone changes and root colonization strategies are highly host-specific[J]. Plant Signaling & Behavior, 2019, 14(9): 1632688.

基因 Gene	引物名称 Primer name	引物序列(5'-3') Primer sequences (5'-3')
OsIAA13	OsIAA13-qF	CAACAGCTACAAGGACCTCTC
	OsIAA13-qR	ACAAGCATCCAGTCACCATC
OsMADS25	OsMADS25-qF	GCGGGAGGTTACAACTTTGAG
	OsMADS25-qR	TTCTCCAGAGTTAAGCTTCTTGTG
OsPTR9	OsPTR9-qF	GACAACACTGAGGAGAGATGC
	OsPTR9-qR	ACAGGCCAAGAAACAGGATG
OsYUCCA1	OsYUCCA1-qF	ACGCTAGACCACATCAAATCC
	OsYUCCA1-qR	TTGCCATCCGTGAACCTG
OsActin	OsActin-qF	TTCCAGCCTTCCTTCATA
	OsActin-qR	AACGATGTTGCCATATAGAT

基因 Gene	引物名称 Primer name	引物序列(5'-3') Primer sequences (5'-3')
OsIAA13	OsIAA13-qF	CAACAGCTACAAGGACCTCTC
	OsIAA13-qR	ACAAGCATCCAGTCACCATC
OsMADS25	OsMADS25-qF	GCGGGAGGTTACAACTTTGAG
	OsMADS25-qR	TTCTCCAGAGTTAAGCTTCTTGTG
OsPTR9	OsPTR9-qF	GACAACACTGAGGAGAGATGC
	OsPTR9-qR	ACAGGCCAAGAAACAGGATG
OsYUCCA1	OsYUCCA1-qF	ACGCTAGACCACATCAAATCC
	OsYUCCA1-qR	TTGCCATCCGTGAACCTG
OsActin	OsActin-qF	TTCCAGCCTTCCTTCATA
	OsActin-qR	AACGATGTTGCCATATAGAT

品种 Variety	秧龄 Seedling age(d)	处理 Treatment	叶龄 Leaf age	株高 Plant height (cm)	根长 Root length (cm)	叶面积 Leaf area (cm²)	茎基宽 Stem base width (mm)
TY203	14	CK	2.30±0.34 b	16.61±1.34 c	5.48±0.57 b	2.12±0.34 c	1.23±0.06 b
		P	2.46±0.42 ab	17.84±1.05 b	7.48±0.51 a	2.68±0.56 b	1.30±0.10 b
		F	2.60±0.25 ab	18.93±0.56 a	7.07±1.16 a	3.04±0.49 ab	1.53±0.06 a
		FP	2.70±0.28 a	18.71±0.32 a	7.52±0.89 a	3.42±0.46 a	1.57±0.06 a
	22	CK	2.83±0.46 b	17.53±0.47 c	6.91±0.53 c	3.19±0.50 c	1.57±0.06 c
		P	3.48±0.39 a	19.39±0.92 b	9.22±0.69 ab	4.63±0.70 b	1.80±0.10 b
		F	3.51±0.45 a	20.68±0.80 a	8.73±0.54 b	5.16±0.77 ab	1.83±0.06 b
		FP	3.60±0.40 a	21.01±0.40 a	9.59±0.84 a	5.45±0.99 a	1.97±0.06 a
	30	CK	3.72±0.42 b	19.17±0.83 d	8.50±0.48 c	4.28±0.69 b	2.10±0.01 c
		P	4.22±0.34 a	21.31±0.59 c	11.13±0.66 a	5.81±0.99 a	2.20±0.10 c
		F	4.27±0.41 a	23.06±0.37 b	7.57±0.56 d	6.17±1.61 a	2.37±0.06 b
		FP	4.38±0.33 a	24.51±0.54 a	10.24±0.56 b	6.88±1.28 a	2.53±0.06 a
ZY246	14	CK	2.10±0.12 c	13.04±0.65 c	6.21±0.27 d	1.32±0.44 c	1.33±0.06 c
		P	2.40±0.17 b	13.98±0.73 b	8.59±0.41 a	1.75±0.24 b	1.47±0.06 b
		F	2.74±0.29 a	16.35±0.86 a	7.66±0.63 c	2.01±0.40 ab	1.63±0.06 a
		FP	2.93±0.34 a	16.68±1.04 a	8.12±0.45 b	2.31±0.38 a	1.73±0.06 a
	22	CK	3.03±0.09 b	14.21±0.83 c	7.49±0.82 d	2.38±0.15 c	1.53±0.06 b
		P	3.42±0.44 a	15.86±0.88 b	10.08±0.87 a	3.03±0.49 b	1.70±0.10 b
		F	3.45±0.34 a	18.27±0.68 a	8.30±0.49 c	3.51±0.43 a	2.00±0.01 a
		FP	3.67±0.33 a	18.64±0.74 a	9.06±0.67 b	3.97±0.83 a	2.17±0.23 a
	30	CK	3.64±0.39 c	16.84±0.78 d	8.83±0.46 c	3.65±0.36 b	1.87±0.06 d
		P	4.06±0.54 b	19.37±0.58 c	12.95±0.58 a	4.41±1.34 b	2.13±0.12 c
		F	4.27±0.37 ab	20.98±1.19 b	8.03±0.64 d	5.29±0.77 a	2.37±0.15 b
		FP	4.48±0.40 a	22.54±0.52 a	11.82±0.82 b	5.57±0.68 a	2.60±0.10 a

品种 Variety	秧龄 Seedling age(d)	处理 Treatment	叶龄 Leaf age	株高 Plant height (cm)	根长 Root length (cm)	叶面积 Leaf area (cm²)	茎基宽 Stem base width (mm)
TY203	14	CK	2.30±0.34 b	16.61±1.34 c	5.48±0.57 b	2.12±0.34 c	1.23±0.06 b
		P	2.46±0.42 ab	17.84±1.05 b	7.48±0.51 a	2.68±0.56 b	1.30±0.10 b
		F	2.60±0.25 ab	18.93±0.56 a	7.07±1.16 a	3.04±0.49 ab	1.53±0.06 a
		FP	2.70±0.28 a	18.71±0.32 a	7.52±0.89 a	3.42±0.46 a	1.57±0.06 a
	22	CK	2.83±0.46 b	17.53±0.47 c	6.91±0.53 c	3.19±0.50 c	1.57±0.06 c
		P	3.48±0.39 a	19.39±0.92 b	9.22±0.69 ab	4.63±0.70 b	1.80±0.10 b
		F	3.51±0.45 a	20.68±0.80 a	8.73±0.54 b	5.16±0.77 ab	1.83±0.06 b
		FP	3.60±0.40 a	21.01±0.40 a	9.59±0.84 a	5.45±0.99 a	1.97±0.06 a
	30	CK	3.72±0.42 b	19.17±0.83 d	8.50±0.48 c	4.28±0.69 b	2.10±0.01 c
		P	4.22±0.34 a	21.31±0.59 c	11.13±0.66 a	5.81±0.99 a	2.20±0.10 c
		F	4.27±0.41 a	23.06±0.37 b	7.57±0.56 d	6.17±1.61 a	2.37±0.06 b
		FP	4.38±0.33 a	24.51±0.54 a	10.24±0.56 b	6.88±1.28 a	2.53±0.06 a
ZY246	14	CK	2.10±0.12 c	13.04±0.65 c	6.21±0.27 d	1.32±0.44 c	1.33±0.06 c
		P	2.40±0.17 b	13.98±0.73 b	8.59±0.41 a	1.75±0.24 b	1.47±0.06 b
		F	2.74±0.29 a	16.35±0.86 a	7.66±0.63 c	2.01±0.40 ab	1.63±0.06 a
		FP	2.93±0.34 a	16.68±1.04 a	8.12±0.45 b	2.31±0.38 a	1.73±0.06 a
	22	CK	3.03±0.09 b	14.21±0.83 c	7.49±0.82 d	2.38±0.15 c	1.53±0.06 b
		P	3.42±0.44 a	15.86±0.88 b	10.08±0.87 a	3.03±0.49 b	1.70±0.10 b
		F	3.45±0.34 a	18.27±0.68 a	8.30±0.49 c	3.51±0.43 a	2.00±0.01 a
		FP	3.67±0.33 a	18.64±0.74 a	9.06±0.67 b	3.97±0.83 a	2.17±0.23 a
	30	CK	3.64±0.39 c	16.84±0.78 d	8.83±0.46 c	3.65±0.36 b	1.87±0.06 d
		P	4.06±0.54 b	19.37±0.58 c	12.95±0.58 a	4.41±1.34 b	2.13±0.12 c
		F	4.27±0.37 ab	20.98±1.19 b	8.03±0.64 d	5.29±0.77 a	2.37±0.15 b
		FP	4.48±0.40 a	22.54±0.52 a	11.82±0.82 b	5.57±0.68 a	2.60±0.10 a

品种 Variety	秧龄 Seedling age(d)	处理 Treatment	叶龄 Leaf age	株高 Plant height	根长 Root length	叶面积 Leaf area	茎基宽 Stem base width
TY203	14	CK (X₁)	0.6571	1.0000	0.7287	0.6199	0.7834
		P (X₂)	0.7029	0.9311	0.9947	0.7836	0.8280
		F (X₃)	0.7429	0.8774	0.9402	0.8889	0.9745
		FP (X₄)	0.7714	0.8878	1.0000	1.0000	1.0000
	22	CK (X₁)	0.8086	1.0000	0.7205	0.5853	0.7970
		P (X₂)	0.9943	0.9041	0.9614	0.8495	0.9137
		F (X₃)	0.9972	0.8477	0.9103	0.9468	0.9289
		FP (X₄)	0.9730	0.8344	1.0000	1.0000	1.0000
	30	CK (X₁)	0.9442	1.0000	0.7637	0.6221	0.8300
		P (X₂)	0.8543	0.8996	1.0000	0.8445	0.8696
		F (X₃)	0.8472	0.8313	0.6801	0.8968	0.9368
		FP (X₄)	0.8327	0.7821	0.9200	1.0000	1.0000
ZY246	14	CK (X₁)	0.6000	1.0000	0.7229	0.5714	0.7688
		P (X₂)	0.6857	0.9328	1.0000	0.7576	0.8497
		F (X₃)	0.7829	0.7976	0.8917	0.8701	0.9422
		FP (X₄)	0.8371	0.7818	0.9453	1.0000	1.0000
	22	CK (X₁)	0.8657	1.0000	0.7431	0.5995	0.7051
		P (X₂)	0.9771	0.8960	1.0000	0.7632	0.7834
		F (X₃)	0.9857	0.7778	0.8234	0.8841	0.9217
		FP (X₄)	0.9557	0.7623	0.8988	1.0000	1.0000
	30	CK (X₁)	0.9630	1.0000	0.6819	0.6553	0.7192
		P (X₂)	0.8788	0.8694	1.0000	0.7917	0.8192
		F (X₃)	0.8472	0.8027	0.6201	0.9497	0.9115
		FP (X₄)	0.8205	0.7471	0.9127	1.0000	1.0000
X₀			1.0000	1.0000	1.0000	1.0000	1.0000