【目的】明确种植方式和施肥对水稻根系形态、生长发育及产量形成的影响，为水稻的种植及科学施肥提供理论依据。【方法】以杂交稻和两优332(HLY332)和常规稻黄华占(HHZ)为材料，采用双因素试验设计，即种植方式(直播和移栽)和施肥(不施肥和施肥)，研究水稻的根系分布、根系形态、根系活力和抗倒伏能力等对种植方式和施肥的响应。【结果】1）与不施肥处理相比，HLY332在直播和移栽条件下，施肥处理的产量分别提高了15.3%和15.0%；HHZ分别提高了18.6%和22.1%。HLY332和HHZ在不同种植方式下的产量均无显著差异。2）直播的根系主要分布在0~5 cm的土层，直播条件下，HLY332不施肥和施肥处理在0~5 cm土层的平均根系生物量占比分别为50.1%和56.8%，HHZ分别为63.5%和67.0%；而移栽水稻的根系则主要分布在5~10 cm的土层，HLY332不施肥和施肥处理在5~10 cm土层的平均根系生物量占比分别为42.9%和41.9%，HHZ分别为53.2%和49.1%。与不施肥处理相比，HLY332直播和移栽条件下，施肥处理的根系生物量分别增加了46.9%和45.9%；HHZ分别增加了64.9%和56.9%。与不施肥处理相比，HLY332直播和移栽条件下，施肥处理的根伤流强度分别增加了113.3%和110.6%；HHZ分别增加了46.1%和60.8%。3）相比于直播，移栽时水稻具有较强的抗折力，与直播处理相比，HLY332不施肥和施肥条件下，移栽处理的抗折力分别增加了23.9%和15.2%；HHZ分别增加了12.5%和17.0%。与不施肥处理相比，HLY332直播和移栽条件下，施肥处理的抗折力分别增加了25.5%和16.8%；HHZ分别增加了23.7%和28.6%。【结论】水稻根系在土层中的分布不同是直播和移栽水稻根系性状差异的显著特征。在本研究条件下，直播或移栽的种植方式下，施肥均能够显著改善根系形态，提高水稻产量，且直播和移栽之间无显著差异。因此，在当前人们对轻简化种植迫切需求的形势下，采用直播种植方式和科学施肥技术相结合是可行的。

【Objective】 To clarify the effects of rice planting methods and fertilization on root morphology, growth and development, and yield formation, and to provide a theoretical basis for rice cultivation and scientific fertilization. 【Method】 A two-factor experimental design was employed to investigate the effects of planting method (direct seeding and transplanting) and fertilization (no fertilization and fertilization) on root distribution, root morphology, root vigor, and lodging resistance in rice. The hybrid rice Heliangyou 332 (HLY332) and conventional rice Huanghuazhan (HHZ) were used as experimental materials. 【Result】 (1) Compared with no fertilization, fertilization significantly increased yield by 15.3% and 15.0% under direct seeding and transplanting conditions for HLY332, and by 18.6% and 22.1% for HHZ, respectively. No significant yield difference was detected between planting methods for either variety. (2) In direct-seeded rice, roots were mainly distributed in the 0-5 cm soil layer. The average root biomass proportion in the 0-5 cm layer under no-fertilizer and fertilizer treatments was 50.1% and 56.8% for HLY332, and 63.5% and 67.0% for HHZ, respectively. In contrast, transplanted rice concentrated roots in the 5-10 cm layer, with proportions of 42.9% and 41.9% for HLY332, and 53.2% and 49.1% for HHZ, respectively. Fertilization increased total root biomass by 46.9% and 45.9% under direct seeding and transplanting for HLY332, and by 64.9% and 56.9% for HHZ, compared to no fertilization. Root bleeding intensity was significantly enhanced by fertilization, with increases of 113.3% and 110.6% for HLY332, and 46.1% and 60.8% for HHZ under direct seeding and transplanting, respectively. (3) Compared with direct seeding, transplanting significantly improved bending resistance. Bending resistance was increased by 23.9% and 15.2% in HLY332 under no-fertilizer and fertilized conditions, and by 12.5% and 17.0% in HHZ, respectively. Fertilization also enhanced bending resistance, with increases of 25.5% and 16.8% for HLY332 under direct seeding and transplanting, and 23.7% and 28.6% for HHZ under transplanting, relative to no fertilization. 【Conclusion】 The distinct vertical distribution of roots in the soil profile is a key trait differentiating direct-seeded and transplanted rice. Under the conditions of this study, fertilization significantly improved root morphology and increased grain yield in both planting methods, with no significant yield difference between direct seeding and transplanting. Therefore, integrating direct seeding with scientific fertilization represents a viable approach to meet the growing demand for light and simplified cultivation in rice production.