水稻抗白叶枯病新基因Xa49(t)的定位分析
收稿日期: 2024-03-04
修回日期: 2024-04-10
网络出版日期: 2025-11-19
基金资助
科技创新2030-重大项目(2022ZD0400203);国家自然科学基金资助项目(32072412);国家自然科学基金资助项目(32101697);四川省科技计划资助项目-重点研发项目(2021YFN0003)
Mapping Analysis of a New Bacterial Blight Resistance Gene Xa49(t) in Rice
Received date: 2024-03-04
Revised date: 2024-04-10
Online published: 2025-11-19
【目的】白叶枯病是水稻生产上的重要病害之一，培育和推广种植抗病品种是防治该病害最理想的措施，而挖掘广谱抗病基因是培育抗病新品种的关键。【方法】野生稻为水稻育种提供了丰富的遗传资源。本研究前期通过普通野生稻(Oryza rufipogon)和籼稻IR24杂交创制了新的野生稻导入系W6023。经抗谱鉴定和遗传群体分析，发现W6023携带一个新的白叶枯病抗性基因，将其暂命名为Xa49(t)。通过构建IR24×W6023的F2群体，运用BSA关联分析，结合新设计的InDel、SNP标记对目标基因Xa49(t)进行初定位。【结果】在水稻11号染色体上鉴定到13个与Xa49(t)基因抗性连锁的分子标记，最终将其定位到D11-111和D11-241间的区域内。【结论】广谱抗白叶枯病新基因Xa49(t)被初步定位于11号染色体968 kb的区间内。
邓欢, 刘亚培, 王春连, 郭威, 陈析丰, 纪志远 . 水稻抗白叶枯病新基因Xa49(t)的定位分析[J]. 中国水稻科学, 2025 , 39(6) : 825 -831 . DOI: 10.16819/j.1001-7216.2025.240302
【Objective】 Bacterial blight (BB) is one of the most serious diseases in rice. Breeding and planting resistant varieties is the most efficient strategy for disease control, and exploring resistance (R) genes is key to developing BB-resistant varieties. 【Method】 Wild rice provides valuable genetic resources for rice breeding. In this study, an introgression line, W6023, derived from a cross between Oryza rufipogon and Oryza sativa (IR24), was used. Genetic and resistance spectrum analyses revealed that W6023 carries a novel dominant BB R gene, tentatively named Xa49(t). An F₂ segregating population from IR24 × W6023 was constructed, and bulk segregant analysis (BSA) combined with newly designed InDel and SNP markers was applied for preliminary mapping of the target gene Xa49(t). 【Result】 Thirteen molecular markers linked to Xa49(t) were identified on chromosome 11, and the gene was ultimately delimited to a region between markers D11-111 and D11-241. 【Conclusion】 The novel broad-spectrum BB R gene Xa49(t) was preliminarily mapped to an approximately 968 kb region on rice chromosome 11.
Key words： rice; bacterial blight; Xa49(t); gene mapping
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