水稻OsRDR5基因功能及作用机制初步解析
收稿日期: 2025-02-24
修回日期: 2025-03-28
网络出版日期: 2025-11-19
基金资助
江西省农业科学院基础研究与人才培养项目(JXSNKYJCRC202507)；江西省财政厅单产提升项目(2024JXCZ001)；赣鄱俊才支持计划资助项目(20243BCE51058)；水稻种质创新与育种江西省重点实验室项目(20241ZDD02034)；农业农村部粮食作物基因资源评价利用重点实验室开放课题(粮基因重(沪)(2024)第1号)。
Preliminary Analysis of Function and Mechanism of OsRDR5 Gene in Rice
Received date: 2025-02-24
Revised date: 2025-03-28
Online published: 2025-11-19
【目的】干旱是影响水稻生产的重要因素，避旱性是作物抗旱最重要的机制。研究水稻避旱性候选基因OsRDR5的作用机制，可以为水稻避旱性的改良提供理论依据。【方法】利用NCBI数据库对OsRDR5核酸序列、蛋白结构域和系统进化关系进行分析；采用qRT-PCR进行组织表达模式分析；通过CRISPR/Cas9系统构建OsRDR5基因敲除突变体；采用“篮子法”评价OsRDR5对水稻深根比的影响。【结果】OsRDR5具有一个Coa3_cc结构域，属于细胞色素c氧化酶组装因子3基因家族，定位于内质网，在水稻根、茎、叶等主要组织中均有表达。深根比表型鉴定发现，野生型深根比为53.7%，突变体株系深根比分别为63.3%和64.9%，显著高于野生型。根系转录组分析共鉴定到1434个差异表达基因。GO富集分析表明，这些基因显著富集到响应缺水、脱落酸、活性氧等与干旱胁迫相关的通路。在缺水通路中，多个与干旱胁迫相关的NAC基因(OsNAC016、OsNAC45等)上调表达。【结论】OsRDR5基因敲除影响了水稻深根比，在水稻避旱性中发挥重要作用。
侯桂花, 周立国, 雷建国, 陈虹, 聂元元 . 水稻OsRDR5基因功能及作用机制初步解析[J]. 中国水稻科学, 2025 , 39(6) : 779 -788 . DOI: 10.16819/j.1001-7216.2025.250208
【Objective】 Drought is a major constraint on rice production, and drought avoidance is the most important mechanism for drought resistance of crops. Understanding the mechanism of OsRDR5, a candidate gene of drought tolerance in rice, can provide a theoretical basis for the improvement of drought tolerance in rice.【Method】 The nucleotide sequence, protein domain and phylogenetic relationship of RDR5 were analyzed using the NCBI database. The tissue expression pattern was analyzed by qRT-PCR. An OsRDR5 gene knockout mutant was constructed by the CRISPR/Cas9 system. The effect of OsRDR5 on rice deep root ratio was evaluated by the "basket method".【Result】OsRDR5 has a Coa3_cc domain, belonging to the cytochrome c oxidase assembly factor 3 gene family. OsRDR5 is located in the endoplasmic reticulum and is expressed in roots, stems and leaves of rice. Phenotypic identification of deep root ratio showed that the wild type had a deep root ratio of 53.7%, while the mutant lines had a deep root ratio of 63.3% and 64.9%, respectively. The deep root ratio of the mutant was significantly increased. A total of 1434 differentially expressed genes were identified by root transcriptomic analysis. GO enrichment analysis revealed significant enrichment of these genes in pathways related to drought stress, including response to water deprivation, abscisic acid and reactive oxygen species, among which several NAC genes(OsNAC016, OsNAC45, etc.) related to drought stress were up-regulated in the water deprivation pathway.【Conclusion】OsRDR5 knockout affects the deep root ratio of rice and plays an important role in drought avoidance of rice.
Key words： rice; OsRDR5; gene function; drought avoidance
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