利用CBE系统编辑Wx基因调控稻米直链淀粉含量
#共同第一作者
收稿日期: 2024-07-07
修回日期: 2024-10-23
网络出版日期: 2025-11-19
基金资助
浙江省自然科学基金资助项目(LQ20C130010);国家水稻产业技术体系（山东省农业良种工程）资助项目(2024LZGCQY007);山东省科技型中小企业创新能力提升工程资助项目(2024TSGC0841);山东省水稻产业技术体系资助项目(SDAIT-17-17)
Regulation of Amylose Content in Rice by Editing Wx Using CBE Base Editor
Received date: 2024-07-07
Revised date: 2024-10-23
Online published: 2025-11-19
【目的】Wx基因编码颗粒淀粉合成酶，是控制直链淀粉合成的主效基因，对稻米中直链淀粉的含量起决定性作用，直接影响稻米品质性状。为了实现小幅降低稻米直链淀粉含量，并为水稻品质改良提供一种新的途径。【方法】利用单碱基编辑器CBE系统对圣稻18的Wx基因第一外显子进行碱基替换，获得了两种碱基替换类型GCCCCC→GCCCTT和GCCCCC→GCTCTC，均造成颗粒淀粉合成酶Wx第94位脯氨酸替换成亮氨酸，分别命名为WxP94L-1和WxP94L-2。【结果】田间实验表明，相比野生型，WxP94L-1和WxP94L-2植株在株高、分蘖数、穗长、粒型、千粒重等主要农艺性状上无显著差异。稻米品质分析发现，WxP94L-1和WxP94L-2植株稻米直链淀粉含量由野生型的14.9%下降至12.5%和12.1%，同时胶稠度、碱消值和蛋白含量等性状与野生型相比无显著改变。【结论】总之，本研究利用单碱基编辑器创制了Wx基因新的等位型WxP94L，该等位型可使稻米直链淀粉含量小幅度降低，为水稻品质改良提供了一种新途径。
陈玉叶, 焦晓真, 王健, 王克剑, 陈峰, 朱克明, 刘朝雷 . 利用CBE系统编辑Wx基因调控稻米直链淀粉含量[J]. 中国水稻科学, 2025 , 39(6) : 771 -778 . DOI: 10.16819/j.1001-7216.2025.240705
【Objective】 The Wx gene, a key regulator of amylose synthesis in rice, encodes a granule-bound starch synthase and plays a crucial role in determining the amylose content, thereby directly influencing the quality traits of rice. 【Method】 We used the CBE base editor to introduce base substitutions within the first exon of the Wx gene in Shendao18 variety, resulting in two distinct mutation types: GCCCCC→GCCCTT and GCCCCC→GCTCTC. Both mutations led to the substitution of proline with leucine at position 94 of the Wx protein, designated as WxP94L-1 and WxP94L-2 respectively. 【Result】 Field experiments demonstrated that compared to the wild type, both WxP94L-1 and WxP94L-2 plants exhibited no significant differences in plant height, tiller number per plant, panicle length, grain morphology or 1000-grain weight. Rice quality analysis revealed that the amylose content decreased from 14.9% (wild type) to 12.5% (WxP94L-1) and 12.1% (WxP94L-2), while traits such as viscosity, alkali spreading value and protein content showed no significant change compared to the wild type. 【Conclusion】 Our study successfully generated a novel allele of the Wx gene named WxP94L using CBE base editor which resulted in a slight reduction in amylose content; thus providing a way for enhancing rice quality.
Key words： Wx; amylose content; base editing; CBE
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