水稻泛素结合酶E2的生物学功能研究进展
收稿日期: 2025-03-24
修回日期: 2025-09-19
网络出版日期: 2025-11-19
基金资助
中央级公益性科研院所基本科研业务费专项(CPSIBRF-CNRRI-202305);中国农业科学院科技创新工程项目
Research Progress on Biological Functions of Ubiquitin-conjugating Enzymes in Rice
Received date: 2025-03-24
Revised date: 2025-09-19
Online published: 2025-11-19
王娟, 吴丽娟, 洪海波, 姚志文, 王磊, 鄂志国 . 水稻泛素结合酶E2的生物学功能研究进展[J]. 中国水稻科学, 2025 , 39(6) : 744 -750 . DOI: 10.16819/j.1001-7216.2025.250316
Ubiquitination is a critical post-translational modification, accounting for 80%-85% of protein degradation in eukaryotes. It regulates protein activity, localization, and function, thereby playing extensive roles in modulating diverse biological processes. The ubiquitin-conjugating enzyme E2, the second key enzyme in the ubiquitination cascade, acts not only as a ubiquitin carrier but also determines the type of ubiquitin linkages and the length of ubiquitin chains. A total of 48 E2 genes have been identified in rice, all encoding proteins that contain a highly conserved UBC (ubiquitin-conjugating) domain. This domain binds to ubiquitin via a thioester bond and forms a specific interaction interface with ubiquitin ligase E3, ensuring the precision of ubiquitin transfer. Studies have shown that E2 enzymes are widely involved in regulating rice growth and development, as well as responses to biotic stresses such as pathogen infection and abiotic stresses including drought and cold.
Key words： ubiquitin-conjugating enzyme; ubiquitination; protein modification; rice
| [1] | Wang J, Jiang J, Oard J H. Structure, expression and promoter activity of two polyubiquitin genes from rice (Oryza sativa L.)[J]. Plant Science, 2000, 156(2): 201-211. |
| [2] | Morreale F E, Walden H. Types of ubiquitin ligases[J]. Cell, 2016, 165(1): 248-248.e1. |
| [3] | Yang Q, Zhao J, Chen D, Wang Y. E3 ubiquitin ligases: Styles, structures and functions[J]. Molecular Biomedicine, 2021, 2(1): 23. |
| [4] | Mevissen T E T, Hospenthal M K, Geurink P P, Elliott P R, Akutsu M, Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund S M V, Ovaa H, Komander D. OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis[J]. Cell, 2013, 154(1): 169-184. |
| [5] | Zhang Y, Li T, Wang L, Zhao H. Characterization of the ubiquitin-conjugating enzyme gene family in rice and evaluation of expression profiles under abiotic stresses and hormone treatments[J]. PLoS One, 2015, 10(4): e0122621. |
| [6] | Bae H, Kim W T. Classification and interaction modes of 40 rice E 2 ubiquitin-conjugating enzymes with 17 rice ARM-U-box E3 ubiquitin ligases[J]. Biochemical and Biophysical Research Communications, 2014, 444(4): 575-580. |
| [7] | Zang Y, Wang Q, Xue C, Li M, Wen R, Xiao W. Rice UBC13 a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage[J]. Rice, 2012, 5(1): 24. |
| [8] | Wang Q, Zang Y, Zhou X, Xiao W. Characterization of four rice UEV1 genes required for Lys63-linked polyubiquitination and distinct functions[J]. BMC Plant Biology, 2017, 17(1): 126. |
| [9] | Wang F, Deng M, Chen J, He Q, Jia X, Guo H, Xu J, Liu Y, Zhang S, Shou H, Mao C. CASEIN KINASE2-dependent phosphorylation of PHOSPHATE2 fine-tunes phosphate homeostasis in rice[J]. Plant Physiology, 2020, 183(1): 250-262. |
| [10] | Wang R, You X, Zhang C, Fang H, Wang M, Zhang F, Kang H, Xu X, Liu Z, Wang J, Zhao Q, Wang X, Hao Z, He F, Tao H, Wang D, Wang J, Fang L, Qin M, Zhao T, Zhang P, Xing H, Xiao Y, Liu W, Xie Q, Wang G L, Ning Y. An ORFeome of rice E3 ubiquitin ligases for global analysis of the ubiquitination interactome[J]. Genome Biology, 2022, 23(1): 154. |
| [11] | Liu H, Liu S, Yu H, Huang X, Wang Y, Jiang L, Meng X, Liu G, Chen M, Jing Y, Yu F, Wang B, Li J. An engineered platform for reconstituting functional multisubunit SCF E3 ligase in vitro[J]. Molecular Plant, 2022, 15(8): 1285-1299. |
| [12] | Swatek K N, Komander D. Ubiquitin modifications[J]. Cell Research, 2016, 26(4): 399-422. |
| [13] | Han Y, Zhang C, Sha H, Wang X, Yu Y, Liu J, Zhao G, Wang J, Qiu G, Xu X, Fang J. Ubiquitin-conjugating enzyme OsUBC11 affects the development of roots via auxin pathway[J]. Rice, 2023, 16(1): 9. |
| [14] | Zhang C, Wang H, Tian X, Lin X, Han Y, Han Z, Sha H, Liu J, Liu J, Zhang J, Bu Q, Fang J. A transposon insertion in the promoter of OsUBC12 enhances cold tolerance during japonica rice germination[J]. Nature Communications, 2024, 15(1): 2211 |
| [15] | Li J, Zhang B, Duan P, Yan L, Yu H, Zhang L, Li N, Zheng L, Chai T, Xu R, Li Y. An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice[J]. The Plant Cell, 2023, 35(3): 1076-1091. |
| [16] | Zheng Y, Zhang S, Luo Y, Li F, Tan J, Wang B, Zhao Z, Lin H, Zhang T, Liu J, Liu X, Guo J, Xie X, Chen L, Liu Y G, Chu Z. Rice OsUBR7 modulates plant height by regulating histone H2B monoubiquitination and cell proliferation[J]. Plant Communications, 2022, 3(6): 100412. |
| [17] | Liu J, Liao W, Nie B, Zhang J, Xu W. OsUEV1B, an Ubc enzyme variant protein, is required for phosphate homeostasis in rice[J]. The Plant Journal, 2021, 106(3): 706-719. |
| [18] | Hu B, Zhu C, Li F, Tang J, Wang Y, Lin A, Liu L, Che R, Chu C. LEAF TIP NECROSIS1 plays a pivotal role in the regulation of multiple phosphate starvation pesponses in rice[J]. Plant Physiology, 2011, 156(3): 1101-1115. |
| [19] | Cao Y, Yan Y, Zhang F, Wang H D, Gu M, Wu X N, Sun S B, Xu G H. Fine characterization of OsPHO2 knockout mutants reveals its key role in Pi utilization in rice[J]. Journal of Plant Physiology, 2014, 171(3-4): 340-348. |
| [20] | Hu B, Wang W, Deng K, Li H, Zhang Z, Zhang L, Chu C. MicroRNA399 is involved in multiple nutrient starvation responses in rice[J]. Frontiers in Plant Science, 2015, 6: 188. |
| [21] | Yamamoto T, Mori Y, Ishibashi T, Uchiyama Y, Sakaguchi N, Furukawa T, Hashimoto J, Kimura S, Sakaguchi K. Characterization of Rad 6 from a higher plant, rice (Oryza sativa L.) and its interaction with Sgt1, a subunit of the SCF ubiquitin ligase complex[J]. Biochemical and Biophysical Research Communications, 2004, 314(2): 434-439. |
| [22] | Zang G, Zou H, Zhang Y, Xiang Z, Huang J, Luo L, Wang C, Lei K, Li X, Song D, Din A U, Wang G. The de-etiolated 1 homolog of Arabidopsis modulates the ABA signaling pathway and ABA biosynthesis in rice[J]. Plant Physiology, 2016, 171(2): 1259-1276. |
| [23] | Kim B, Lee Y, Nam J Y, Lee G, Seo J, Lee D, Cho Y H, Kwon S W, Koh H J. Mutations in OsDET1, OsCOP10, and OsDDB1 confer embryonic lethality and alter flavonoid accumulation in rice (Oryza sativa L.) seed[J]. Frontiers in Plant Science, 2022, 13: 952856. |
| [24] | Wei Z, Zhang Y, Yuan Y, Li L, Li T, Guan Y, Wang D, Gao Y, Gao Q, Ji J, Nguyen T, Liu X. Ubiquitin-conjugated enzyme OsUBC16 negatively regulates grain size and grain weight in rice[J]. Journal of Plant Biology, 2024, 67(5): 409-418. |
| [25] | Xie Y, Fan Z, Liang X, Teng K, Huang Z, Huang M, Zhao H, Xu K, Li J. OsPUB9 modulates leaf angle and grain size through the brassinosteroid signaling pathway in rice[J]. The Plant Journal, 2025, 121(3): e17230. |
| [26] | Ma J, Wang Y, Ma X, Meng L, Jing R, Wang F, Wang S, Cheng Z, Zhang X, Jiang L, Wang J, Wang J, Zhao Z, Guo X, Lin Q, Wu F, Zhu S, Wu C, Ren Y, Lei C, Zhai H, Wan J. Disruption of gene SPL35 encoding a novel CUE domain-containing protein, leads to cell death and enhanced disease response in rice[J]. Plant Biotechnology Journal, 2019, 17(8): 1679-1693. |
| [27] | Takai R, Matsuda N, Nakano A, Hasegawa K, Akimoto C, Shibuya N, Minami E. EL5, a rice N-acetylchitooligosaccharide elicitor-responsive RING-H2 finger protein, is a ubiquitin ligase which functions in vitro in co-operation with an elicitor-responsive ubiquitin-conjugating enzyme, OsUBC5b[J]. The Plant Journal, 2002, 30(4): 447-455. |
| [28] | Liu J, Nie B, Yu B, Xu F, Zhang Q, Wang Y, Xu W. Rice ubiquitin-conjugating enzyme OsUbc13 negatively regulates immunity against pathogens by enhancing the activity of OsSnRK1a[J]. Plant Biotechnology Journal, 2023, 21(8): 1590-1610. |
| [29] | Filipe O, De Vleesschauwer D, Haeck A, Demeestere K, Höfte M. The energy sensor OsSnRK1a confers broad-spectrum disease resistance in rice[J]. Scientific Reports, 2018, 8(1): 3864. |
| [30] | Cao Y, Lu M, Chen J, Li W, Wang M, Chen F. Identification of Ossnrk1a-1 regulated genes associated with rice immunity and seed set[J]. Plants, 2024, 13(5): 596. |
| [31] | Liu X, Song L, Zhang H, Lin Y, Shen X, Guo J, Su M, Shi G, Wang Z, Lu G D. Rice ubiquitin-conjugating enzyme OsUBC26 is essential for immunity to the blast fungus Magnaporthe oryzae[J]. Molecular Plant Pathology, 2021, 22(12): 1613-1623. |
| [32] | Phan H, Schläppi M. The RAD6-like ubiquitin conjugase gene OsUBC7 has a positive role in the early cold stress tolerance response of rice[J]. Genes, 2025, 16(1): 66. |
| [33] | Pathak B, Maurya C, Faria M C, Alizada Z, Nandy S, Zhao S, Jamsheer K M, Srivastava V. Targeting TOR and SnRK1 genes in rice with CRISPR/Cas9[J]. Plants, 2022, 11(11): 1453. |
| [34] | Ghimire S, Tang X, Liu W, Fu X, Zhang H, Zhang N, Si H. SUMO conjugating enzyme: A vital player of SUMO pathway in plants[J]. Physiology and Molecular Biology of Plants, 2021, 27(10): 2421-2431. |
| [35] | Joo J, Choi D H, Lee Y H, Seo H S, Song S I. The rice SUMO conjugating enzymes OsSCE1 and OsSCE3 have opposing effects on drought stress[J]. Journal of Plant Physiology, 2019, 240 : 152993. |
| [36] | Nurdiani D, Widyajayantie D, Nugroho S. OsSCE1 encoding SUMO E2-conjugating enzyme involves in drought stress response of Oryza sativa[J]. Rice Science, 2018, 25(2): 73-81. |
| [37] | Nigam N, Singh A, Sahi C, Chandramouli A, Grover A. SUMO-conjugating enzyme (Sce) and FK506-binding protein (FKBP) encoding rice (Oryza sativa L.) genes: Genome-wide analysis, expression studies and evidence for their involvement in abiotic stress response[J]. Molecular Genetics and Genomics, 2008, 279(4): 371-383. |
| [38] | Yuan X, Luan Y, Liu D, Wang J, Peng J, Zhao J, Li L, Su J, Xiao Y, Li Y, Ma X, Zhu X, Tan L, Liu F, Sun H, Gu P, Xu R, Zhang P, Zhu Z, Sun C, Fu Y, Zhang K. The SUMO-conjugating enzyme OsSCE1a from wild rice regulates the functional stay-green trait in rice[J]. Plant Biotechnology Journal, 2025, 23(2): 615-631. |
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