不同氮肥水平下水稻倒二叶叶鞘的转录组分析
#共同第一作者
收稿日期: 2025-02-17
修回日期: 2025-04-11
网络出版日期: 2025-07-21
基金资助
浙江省水稻新品种选育重大科技专项(2021C02063-1);浙江省农业科学院重大科技任务攻关项目(2024ZDZX01)
Transcriptome Analysis of Top Second Leaf Sheath of Rice Under Different Nitrogen Fertilizer Levels
＃These authors contributed equally to this work
Received date: 2025-02-17
Revised date: 2025-04-11
Online published: 2025-07-21
【目的】探明叶鞘对氮素响应的重要基因，为水稻氮素高效利用的遗传改良提供科学依据。【方法】将粳稻品种秀水134种植在低氮(LN)、中氮(MN)和高氮(HN)的渗漏池中，选取抽穗后第10天的倒二叶叶鞘进行转录组测序分析，以鉴定叶鞘对氮素响应的重要基因。【结果】转录组数据比较分析显示，三种氮素水平间存在1791个差异表达基因。其中HN与MN、MN与LN、以及HN与LN组间的上调和下调表达基因数分别为312个和155个，263个和160个，1059个和542个。三种施氮水平间的共有上调和下调差异表达基因分别为15个和53个，涉及营养元素吸收和转运、生物与非生物胁迫响应、植物激素响应以及光调控等生理生化过程。基于GO富集分析和KEGG代谢途经分析，最终鉴定出参与氮素利用及光合作用的已知功能基因分别为6个和4个，以及50个功能未知基因，推测这些基因在倒二叶叶鞘的氮素响应中发挥重要作用。【结论】研究结果初步明确了叶鞘对不同氮素水平响应的重要基因，这些基因在氮素利用的信号转导途径中发挥重要作用。
黄福灯, 吴春艳, 郝媛媛, 韩一飞, 张小斌, 孙会锋, 潘刚 . 不同氮肥水平下水稻倒二叶叶鞘的转录组分析[J]. 中国水稻科学, 2025 , 39(4) : 563 -574 . DOI: 10.16819/j.1001-7216.2025.250306
【Objective】 The leaf sheath is an important organ that simultaneously serves the functions of “source, sink, and transport”, playing a crucial role in the growth and development of rice plants. Identifying important genes in leaf sheaths in response to nitrogen will provide scientific evidence for the genetic improvement of nitrogen use efficiency in rice. 【Method】 The japonica rice cultivar Xiushui 134 was planted in percolation ponds at low nitrogen (LN), medium nitrogen (MN), and high nitrogen (HN) levels. The top second leaf sheaths were collected on the 10th day after heading for transcriptome sequencing analysis to identify important genes involved in the nitrogen response of rice leaf sheaths. 【Result】 Comparative analysis of the transcriptome data revealed 1,791 differentially expressed genes at the three nitrogen levels. The number of upregulated and downregulated genes between HN and MN, MN and LN, and HN and LN were 312 and 155, 263 and 160, and 1,059 and 542, respectively. The common upregulated and downregulated genes at the three nitrogen levels were 15 and 53, respectively, involved in physiological and biochemical processes such as nutrient element absorption and transport, responses to biotic and abiotic stresses, plant hormone responses, and light regulation. Based on GO enrichment analysis, KEGG metabolic pathway analysis, a total of 6 known functional genes related to nitrogen utilization and 4 genes related to photosynthesis, as well as 50 genes of unknown function, were identified. It is speculated that these genes play important roles in the nitrogen response of the second leaf sheath in rice. 【Conclusion】 The research results preliminarily clarify the important genes involved in the leaf sheath's response to different nitrogen levels, which may play significant roles in signal transduction pathways of nitrogen utilization.
Key words： rice; top second leaf sheath; nitrogen fertilizer; transcriptome analysis
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