水稻粉质胚乳突变体we1的表型分析与基因定位
收稿日期: 2024-05-15
修回日期: 2024-08-20
网络出版日期: 2025-07-21
基金资助
国家自然科学基金资助项目(32201861);江苏省重点研发计划资助项目(BE2023362);江苏省种业振兴揭榜挂帅项目(JBGS(2021)008)
Phenotypic Analysis and Gene Mapping of a Floury Endosperm Mutant we1 in Rice
Received date: 2024-05-15
Revised date: 2024-08-20
Online published: 2025-07-21
【目的】对粉质胚乳突变体的研究有助于解析稻米品质形成的分子机制。【方法】从日本晴60Co诱变突变体库中筛选到一份粉质皱缩胚乳突变体we1(white endosperm1)，对其表型、理化性质进行分析，并利用we1与9311杂交获得的F2群体对目标基因进行精细定位。【结果】we1胚乳表现为白色粉质皱缩状，淀粉颗粒排列疏松且不规则；千粒重、株高、总淀粉、直链淀粉含量显著下降，脂肪含量显著上升。遗传分析表明we1粉质皱缩胚乳性状受单个隐性基因控制。利用we1/9311 F2群体进行基因定位，WE1被定位在6号染色体短臂P16和P18之间约181 kb区间内，该区间包含23个开放阅读框(Open reading frame, ORFs)。qRT-PCR 结果显示，WE1的突变会影响淀粉合成相关基因的表达。【结论】WE1可能是一个参与淀粉合成的新基因，本研究为进一步解析WE1调控水稻淀粉合成的分子机制奠定了基础。
朱建平, 李霞, 李文奇, 许扬, 王芳权, 陶亚军, 蒋彦婕, 陈智慧, 范方军, 杨杰 . 水稻粉质胚乳突变体we1的表型分析与基因定位[J]. 中国水稻科学, 2025 , 39(4) : 543 -551 . DOI: 10.16819/j.1001-7216.2025.240508
【Objective】 The study of floury endosperm mutants is helpful for elucidating the molecular mechanisms underlying rice grain quality formation. 【Method】 A stably inherited floury endosperm mutant we1 (white endosperm 1) was isolated from a 60Co-irradiated mutant pool of japonica rice cultivar Nipponbare. Phenotypic characterization and physicochemical analyses of we1 were conducted. An F2 population derived from a cross between we1 and indica variety 9311 was used for fine mapping. 【Result】 Compared with the wild type, we1 exhibited white, shrunken endosperm with irregularly shaped and loosely packed compound starch granules. The mutant showed significant reductions in 1000-grain weight, plant height, total starch content, and amylose content, along with a marked increase in lipid content. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene. For map-based cloning, we1 was crossed with 9311 and recessive F2 individuals were selected. The WE1 locus was initially mapped to chromosome 6 and fine-mapped to a 181-kb physical interval. Twenty-three open reading frames (ORFs) were predicted in this region. qRT-PCR analysis revealed altered expression levels of starch synthesis-related genes in the we1 mutant. 【Conclusion】 WE1 likely encodes a novel gene involved in starch synthesis, providing a foundation for further elucidating its molecular regulatory mechanisms in rice.
Key words： rice; floury endosperm; starch; physicochemical property; fine mapping
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