水稻类病斑突变体lm52的基因克隆及其广谱抗病性分析
收稿日期: 2024-03-04
修回日期: 2024-05-06
网络出版日期: 2025-05-21
基金资助
江苏省政府种业振兴计划资助项目[JBGS(2021);国家自然科学基金资助项目(32272110);扬州大学大学生创新创业训练计划项目(XCX20230650);扬州大学“青蓝工程”资助项目
Gene Cloning and Broad-spectrum Disease Resistance Analysis of Rice Lesion Mimic Mutant lm52
Received date: 2024-03-04
Revised date: 2024-05-06
Online published: 2025-05-21
【目的】类病斑突变体(Lesion mimic mutant, LMM)是研究植物细胞死亡和抗病机制的理想材料，可为作物抗病育种提供新的分子靶点和育种材料。【方法】利用图位克隆技术对水稻类病斑突变体lm52的突变基因进行了定位，并利用CRISPR/Cas9敲除技术对候选基因进行了功能验证，同时鉴定了lm52对水稻对主要病害(稻瘟病、纹枯病和白叶枯病)的抗性，并分析其抗性机制。【结果】突变体lm52从4叶期开始逐渐出现褐色点状病斑，成熟期病斑扩展至全叶；ATP酶基因LOC_Os06g03940上的单碱基突变(G1440A)是lm52产生类病斑表型的原因；lm52显著增强了对水稻三大主要病害(稻瘟病、纹枯病和白叶枯病)的抗性，且这与突变体中活氧性(ROS)的过度积累和防御基因的激活表达有关。【结论】ATP酶基因LM52的突变增强了水稻对不同类型病原菌的抗性，为水稻抗病分子育种提供潜在的靶标基因。
黄涛, 魏兆根, 陈玘, 程泽, 刘欣, 王广达, 胡珂鸣, 谢文亚, 陈宗祥, 冯志明, 左示敏 . 水稻类病斑突变体lm52的基因克隆及其广谱抗病性分析[J]. 中国水稻科学, 2025 , 39(3) : 322 -330 . DOI: 10.16819/j.1001-7216.2025.240304
【Objective】Lesion mimic mutants are ideal materials to study the mechanisms of plant cell death and disease resistance, offering new strategies for plant disease resistance breeding.【Method】The lm52 mutant gene was cloned by the map-based cloning method, and the function of the candidate gene was verified by CRISPR/Cas9 knockout technology. Meanwhile, the resistance of lm52 to multiple rice pathogens and the possible resistance mechanisms were analyzed.【Result】The mutant lm52 gradually developed progressive brown spot lesions from the 4th leaf stage, with whole leaf necrosis at maturity stage. A single base mutation (G1440A) on the ATPase encoding gene LOC_Os06g03940 caused the lesion mimic phenotype of lm52. lm52 is characterized by significantly enhanced resistance to blast, sheath blight and bacterial blight, which was found to be related to the overaccumulation of ROS and the activation of defense-related genes.【Conclusion】The mutation of the ATPase gene LM52 enhances rice resistance against diverse pathogens, and provides potential target genes for molecular breeding of rice disease resistance.
Key words： rice; lesion mimic mutant; map-based cloning; disease-resistance
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