不同耐低磷水稻品种的内源激素水平差异及其与产量和磷利用率的关系
收稿日期: 2024-10-12
修回日期: 2025-03-06
网络出版日期: 2025-03-19
基金资助
国家自然科学基金资助项目(32272198);国家自然科学基金资助项目(32071943);国家农业重大科技项目(FSNK202218080316);江苏高校优势学科建设工程资助项目(PAPD-2020-01)
Differences in Endogenous Hormone Levels and Their Relationship with Yield and Phosphorus Use Efficiency in Rice Varieties With Various Tolerance to Low Phosphorus Stress
Received date: 2024-10-12
Revised date: 2025-03-06
Online published: 2025-03-19
【目的】探明不同耐低磷水稻品种内源激素水平的差异及其与产量和磷吸收利用的关系。【方法】以2个强耐低磷品种 (耐低磷指数>0.9)和2个弱耐低磷水稻品种 (耐低磷指数<0.5)为材料，采用水培种植，设置低磷(磷浓度为标准营养液中磷浓度的1/20，0.401 mg/L)和正常磷(8.02 mg/L)两个处理。【结果】与正常磷相比，低磷显著降低了两类品种的产量和磷的吸收量，但提高了磷素干物质生产效率和磷产谷利用率。在低磷处理下，与弱耐低磷品种相比，强耐低磷品种具有较高的产量、磷吸收量、磷利用效率、根干质量、地上部干质量和根系氧化力，根和叶中具有较高的生长素、玉米素及其核苷、脱落酸、茉莉酸类(茉莉酸和茉莉酸甲酯)、油菜素甾醇(24-表油菜素内酯和28-高油菜素内酯)含量，根中具有较高的独脚金内酯含量。这些激素水平与产量、磷吸收利用率、根干质量、地上部干质量和根系氧化力呈显著或极显著正相关；而根和叶中乙烯释放速率和1-氨基环丙烷-1-羧酸含量表现为强耐低磷品种显著低于弱耐低磷品种，并与产量和磷利用率显著或极显著负相关。随机森林分析表明，在各类激素中茉莉酸类和油菜素甾醇对产量和磷利用率的贡献最大。【结论】在低磷胁迫下，强耐低磷品种内源激素之间的平衡，特别是较高的茉莉酸类和油菜素甾醇含量，有利于优化根系形态，维持较高的根系活性，促进磷的吸收和利用，获得较高产量。
徐月梅, 彭诗燕, 孙志伟, 王志琴, 朱宽宇, 杨建昌 . 不同耐低磷水稻品种的内源激素水平差异及其与产量和磷利用率的关系[J]. 中国水稻科学, 2025 , 39(2) : 231 -244 . DOI: 10.16819/j.1001-7216.2025.241004
【Objective】This study aimed to investigate the differences in endogenous hormone levels among rice varieties with varying tolerance to low phosphorus (P) stress and their relationship with grain yield, P uptake, and utilization. 【Method】Two rice varieties with strong tolerance to low P (STVs, low P tolerance index > 0.9) and two varieties with weak tolerance to low P (WTVs, low P tolerance index < 0.5) were grown hydroponically under normal P level (NP, 8.02 mg /L) and low P level (LP, 0.401 mg/ L). 【Results】Compared to NP, LP significantly reduced grain yield and P uptake in both types of rice varieties but increased dry matter production per unit of P and internal P use efficiency. Under LP treatment, the STVs exhibited higher grain yield, P uptake and utilization efficiency, root dry weight, and root oxidation activity compared to the WTVs. Additionally, the STVs had higher content of indole-3-acetic acid, zeatin + zeatin riboside, abscisic acid, jasmonates (jasmonic acid and methyl jasmonate), and brassinosteroids (24-epibrassinolide and 28-homobrassinolide) in both roots and leaves, as well as higher content of strigolactones in roots. These hormone levels were significantly or very significantly positively correlated with grain yield, P uptake and utilization efficiency, root dry weight, and root oxidation activity. Furthermore, a lower ethylene emission rate and lower 1-aminocyclopropane-1-carboxylic acid content were observed in both roots and leaves of the STVs compared to the WTVs, which were significantly or very significantly negatively correlated with grain yield and P use efficiency. Random forest analysis indicated that jasmonates and brassinosteroids served as the predominant contributors to grain yield and P use efficiency. 【Conclusion】Under LP stress, the balance among endogenous hormones, particularly higher levels of jasmonates and brassinosteroids, plays a key role in optimizing root morphology, maintaining higher root activity, promoting P uptake and utilization, and achieving a higher grain yield in STVs.
| [1] | 徐晓明, 张迎信, 王会民, 程式华, 曹立勇. 水稻氮、磷、钾吸收利用遗传特征研究进展[J]. 核农学报, 2016, 30(4): 685-694. |
| Xu X M, Zhang Y X, Wang H M, Cheng S H, Cao L Y. R Advancement of genetic characteristics for N, P and K utilization of rice[J]. Journal of Nuclear Agricultural Sciences, 2016, 30(4): 685-694. (in Chinese with English abstract) | |
| [2] | 李继云, 刘秀娣, 周伟, 孙建华, 童依平, 刘文杰, 李振声, 王培田, 姚树江. 有效利用土壤营养元素的作物育种新技术研究[J]. 中国科学: B辑, 1995(1): 41-48. |
| Li J Y, Liu X D, Zhou W, Sun J H, Tong Y P, Liu W J, Li Z S, Wang P T, Yao S J. Study on new techniques of crop breeding for effective utilization of soil nutrient elements[J]. Science in China: Series B, 1995(1): 41-48. (in Chinese with English abstract) | |
| [3] | 陈健晓, 王小娟, 屠乃美, 王效宁, 符策强, 唐清杰, 刘爱玉. 磷水平对不同香稻品种苗期形态生理的影响[J]. 华北农学报, 2020, 35(2): 117-125. |
| Chen J X, Wang X J, Tu N M, Wang X N, Fu C Q, Tang Q J, Liu A Y. Effects of phosphorus levels on morphological physiology of different fragrant rice varieties at seedling stage[J]. Acta Agriculturae Boreali-Sinica, 2020, 35(2): 117-125. (in Chinese with English abstract) | |
| [4] | 陈磊, 王盛锋, 刘荣乐, 汪洪. 不同磷供应水平下小麦根系形态及根际过程的变化特征[J]. 植物营养与肥料学报, 2012, 18(2): 324-331. |
| Chen L, Wang S F, Liu R L, Wang H. C Changes of root morphology and rhizosphere processes of wheat under different phosphate supply[J]. Journal of Plant Nutrition and Fertilizers, 2012, 18(2): 324-331. (in Chinese with English abstract) | |
| [5] | Weyers J D B, Paterson N W. Plant hormones and the control of physiological processes[J]. New Phytologist, 2001, 152(3): 375-407. |
| [6] | Verma V, Ravindran P, Kumar P P. Plant hormone-mediated regulation of stress responses[J]. BMC Plant Biology, 2016, 16: 86. |
| [7] | Yang Y, Wang L, Zhang D, Che Z, Wang Q, Cui R, Zhao W, Huang F, Zhang H, Cheng H, Yu D. Soybean type-B response regulator GmRR1 mediates phosphorus uptake and yield by modifying root architecture[J]. Plant Physiology, 2024, 194(3): 1527-1544. |
| [8] | Hu D, Cui R, Wang K, Yang Y M, Wang R, Zhu H, He M, Fan Y, Wang L, Chu S, Zhang J, Zhang S, Yang Y, Zhai X, Lv G, Zhang D D, Wang J, Kong F, Yu D, Zhang H Y, Zhang D. The Myb73-GDPD2-GA2ox1 transcriptional regulatory module confers phosphate deficiency tolerance in soybean[J]. The Plant Cell, 2024, 36(6): 2176-2200. |
| [9] | Zhang Y, Li T T, Wang L F, Guo J X, Lu K K, Song R F, Zuo J X, Chen H H, Liu W C. Abscisic acid facilitates phosphate acquisition through the transcription factor ABA INSENSITIVE5 in Arabidopsis[J]. The Plant Journal, 2022, 111(1): 269-281. |
| [10] | Santoro V, Schiavon M, Visentin I, Constán-Aguilar C, Cardinale F, Celi L. Strigolactones affect phosphorus acquisition strategies in tomato plants[J]. Plant, Cell & Environment, 2021, 44(11): 3628-3642. |
| [11] | Ma L, Zhang J, Li Z, Xin X, Guo Z, Wang D, Li D, Zhao B. Long-term phosphorus deficiency decreased bacterial-fungal network complexity and efficiency across three soil types in China as revealed by network analysis[J]. Applied Soil Ecology, 2020, 148: 103506. |
| [12] | Yan J, Lou L, Bai W, Zhang S, Zhang N. Phosphorus deficiency is the main limiting factor for re-vegetation and soil microorganisms in Mu Us Sandy Land, Northwest China[J]. Science of the Total Environment, 2023, 900: 165770. |
| [13] | Deng Y, Men C, Qiao S, Wang W, Gu J, Liu L, Zhang Z, Zhang H, Wang Z, Yang J. Tolerance to low phosphorus in rice varieties is conferred by regulation of root growth[J]. The Crop Journal, 2020, 8(4): 534-547. |
| [14] | Beltrano J, Ronco M G, Montaldi E R. Drought stress syndrome in wheat is provoked by thylene evolution imbalance and reversed by rewatering, aminoethoxyvinylglycine, or sodium benzoate[J]. Journal of Plant Growth Regulation, 1999, 18(2): 59-64. |
| [15] | Cheng C Y, Lur H S. Ethylene may be involved in abortion of the maize caryopsis[J]. Physiologia Plantarum, 1996, 98(2): 245-252. |
| [16] | Bollmark M, Kubát B, Eliasson L. Variation in endogenous cytokinin content during adventitious root formation in pea cuttings[J]. Journal of Plant Physiology, 1988, 132(3): 262-265. |
| [17] | Pan X, Welti R, Wang X. Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometry[J]. Nature Protocols, 2010, 5(6): 986-992. |
| [18] | Liu X, Yang Y L, Lin W H, Tong J, Huang Z, Xiao L. Determination of both jasmonic acid and methyl jasmonate in plant samples by liquid chromatography tandem mass spectrometry[J]. Chinese Science Bulletin, 2010, 55(21): 2231-2235. |
| [19] | Ding J, Mao L J, Yuan B F, Feng Y Q. A selective pretreatment method for determination of endogenous active brassinosteroids in plant tissues: Double layered solid phase extraction combined with boronate affinity polymer monolith microextraction[J]. Plant Methods, 2013, 9: 13. |
| [20] | Chen J, Fei K, Zhang W, Wang Z, Zhang J, Yang J. Brassinosteroids mediate the effect of high temperature during anthesis on the pistil activity of photo- thermosensitive genetic male-sterile rice lines[J]. The Crop Journal, 2021, 9(1): 109-119. |
| [21] | Stewart W M, Dibb D W, Johnston A E, Smyth T J. The contribution of commercial fertilizer nutrients to food production[J]. Agronomy Journal, 2005, 97(1): 1-6. |
| [22] | Irfan M, Shah J A, Abbas M. Evaluating the performance of mungbean genotypes for grain yield, phosphorus accumulation and utilization efficiency[J]. Journal of Plant Nutrition, 2017, 40(19): 2709-2720. |
| [23] | 孙志伟, 徐月梅, 许荣越, 朱宽宇, 杨建昌. 水稻低磷胁迫响应及其调控机制的研究进展[J]. 核农学报, 2023, 37(08): 1562-1570. |
| Sun Z W, Xu Y M, Xu R Y, Zhu K Y, Yang J C. Research advance in the response of rice to low phosphorus stress and its regulation mechanism[J]. Journal of Nuclear Agricultural Sciences, 2023, 37(8): 1562-1570. (in Chinese with English abstract) | |
| [24] | Wang K, Cui K, Liu G, Luo X, Huang J, Nie L, Wei D, Peng S. Low straw phosphorus concentration is beneficial for high phosphorus use efficiency for grain production in rice recombinant inbred lines[J]. Field Crops Research, 2017, 203: 65-73. |
| [25] | Sun Z, Qiao S, Xu Y, Ji D, Zhang W, Gu J, Zhu K, Wang Z, Zhang J, Yang J. Agronomic and physiological performance of the indica rice varieties differing in tolerance to low phosphorus[J]. Agronomy, 2023, 14(1): 41. |
| [26] | Gonçalves B X, Lima-Melo Y, dos Santos Maraschin F, Margis-Pinheiro M. Phosphate starvation responses in crop roots: From well-known players to novel candidates[J]. Environmental and Experimental Botany, 2020, 178: 104162. |
| [27] | Ku Y S, Sintaha M, Cheung M Y, Lam H M. Plant hormone signaling crosstalks between biotic and abiotic stress responses[J]. International Journal of Molecular Sciences, 2018, 19(10): 3206. |
| [28] | Munné-Bosch S, Müller M. Hormonal cross-talk in plant development and stress responses[J]. Frontiers in Plant Science, 2013, 4: 529. |
| [29] | Liu J, Moore S, Chen C, Lindsey K. Crosstalk complexities between auxin, cytokinin, and ethylene in Arabidopsis root development: From experiments to systems modeling, and back again[J]. Molecular Plant, 2017, 10(12): 1480-1496. |
| [30] | Yang J, Zhang J, Wang Z, Zhu Q. Hormones in the grains in relation to sink strength and postanthesis development of spikelets in rice[J]. Plant Growth Regulation, 2003, 41(3): 185-195. |
| [31] | Guo T, Lu Z Q, Shan J X, Ye W W, Dong N Q, Lin H X. ERECTA1 acts upstream of the OsMKKK10- OsMKK4-OsMPK6 cascade to control spikelet number by regulating cytokinin metabolism in rice[J]. The Plant Cell, 2020, 32(9): 2763-2779. |
| [32] | Xu W, Jia L, Shi W, Liang J, Zhou F, Li Q, Zhang J. Abscisic acid accumulation modulates auxin transport in the root tip to enhance proton secretion for maintaining root growth under moderate water stress[J]. New Phytologist, 2013, 197(1): 139-150. |
| [33] | Janicka-Russak M, Kabała K, Wdowikowska A, Kłobus G. Modification of plasma membrane proton pumps in cucumber roots as an adaptation mechanism to salt stress[J]. Journal of Plant Physiology, 2013, 170(10): 915-922. |
| [34] | Miyoshi Y, Soma F, Yin Y G, Suzui N, Noda Y, Enomoto K, Nagao Y, Yamaguchi M, Kawachi N, Yoshida E, Tashima H, Yamaya T, Kuya N, Teramoto S, Uga Y. Rice immediately adapts the dynamics of photosynthates translocation to roots in response to changes in soil water environment[J]. Frontiers in Plant Science, 2022, 13: 1024144. |
| [35] | Gu J, Li Z, Mao Y, Struik P C, Zhang H, Liu L, Wang Z, Yang J. Roles of nitrogen and cytokinin signals in root and shoot communications in maximizing of plant productivity and their agronomic applications[J]. Plant Science, 2018, 274: 320-331. |
| [36] | Janicka-Russak M, Kabała K, Wdowikowska A, Kłobus G. Modification of plasma membrane proton pumps in cucumber roots as an adaptation mechanism to salt stress[J]. Journal of Plant Physiology, 2013, 170(10): 915-922. |
| [37] | Yang J, Zhang J, Wang Z, Zhu Q, Wang W. Hormonal changes in the grains of rice subjected to water stress during grain filling[J]. Plant Physiology, 2001, 127(1): 315-323. |
| [38] | Zhang H, Tan G, Yang L, Yang J, Zhang J, Zhao B. Hormones in the grains and roots in relation to post-anthesis development of inferior and superior spikelets in japonica/indica hybrid rice[J]. Plant Physiology and Biochemistry, 2009, 47(3): 195-204. |
| [39] | Sekhar S, Panda B B, Mohapatra T, Das K, Shaw B P, Kariali E, Mohapatra P K. Spikelet-specific variation in ethylene production and constitutive expression of ethylene receptors and signal transducers during grain filling of compact- and lax-panicle rice (Oryza sativa) cultivars[J]. Journal of Plant Physiology, 2015, 179: 21-34. |
| [40] | Panda B B, Kariali E, Panigrahi R, Mohapatra P K. High ethylene production slackens seed filling in compact panicled rice cultivar[J]. Plant Growth Regulation, 2009, 58(2): 141-151. |
| [41] | Yang J C, Zhang J H, Liu K, Wang Z, Liu L. Abscisic acid and ethylene interact in rice spikelets in response to water stress during meiosis[J]. Journal of Plant Growth Regulation, 2007, 26(4): 318-328. |
| [42] | Zhu X F, Zhu C Q, Zhao X S, Zheng S J, Shen R F. Ethylene is involved in root phosphorus remobilization in rice (Oryza sativa L.) by regulating cell-wall pectin and enhancing phosphate translocation to shoots[J]. Annals of Botany, 2016, 118(4): 645-653. |
| [43] | Howe G A, Major I T, Koo A J. Modularity in jasmonate signaling for multistress resilience[J]. Annual Review of Plant Biology, 2018, 69: 387-415. |
| [44] | Zou L, Qu M, Zeng L, Xiong G. The molecular basis of the interaction between Brassinosteroid induced and phosphorous deficiency induced leaf inclination in rice[J]. Plant Growth Regulation, 2020, 91(2): 263-276. |
| [45] | Muzaffar A, Chen Y S, Lee H T, Wu C C, Le T T, Liang J Z, Lu C H, Balasubramaniam H, Lo S F, Yu L C, Chan C H, Chen K T, Lee M H, Hsing Y I, Ho T D, Yu S M. A newly evolved rice-specific gene JAUP1 regulates jasmonate biosynthesis and signalling to promote root development and multi-stress tolerance[J]. Plant Biotechnology Journal, 2024, 22(5): 1417-1432 |
| [46] | Chen Y, Jin G, Liu M, Wang L, Lou Y, Baldwin I, Li R. Multiomic analyses reveal key sectors of jasmonate- mediated defense responses in rice[J]. The Plant Cell, 2024, 36(9): 3362-3377. |
| [47] | 王黎明, 杨瑞珍, 孙加强. 油菜素内酯调控作物农艺性状和非生物胁迫响应的研究进展[J]. 生物工程学报, 2022, 38(1): 34-49. |
| Wang L M, Yang R Z, Sun J Q. Regulation of crop agronomic traits and abiotic stress responses by brassinosteroids: A review[J]. Chinese Journal of Biotechnology, 2022, 38(1): 34-49. (in Chinese with English abstract) | |
| [48] | Gupta A, Rico-Medina A, Caño-Delgado A I. The physiology of plant responses to drought[J]. Science, 2020, 368(6488): 266-269. |
| [49] | Sun H, Li W, Burritt D J, Tian H, Zhang H, Liang X, Miao Y, Mostofa M G, Tran L P. Strigolactones interact with other phytohormones to modulate plant root growth and development[J]. The Crop Journal, 2022, 10(6): 1517-1527. |
| [50] | Li L, Ge S, He L, Liu R, Mei Y, Xia X, Yu J, Zhou Y. SlDELLA interacts with SlPIF4 to regulate arbuscular mycorrhizal symbiosis and phosphate uptake in tomato[J]. Horticulture Research, 2024, 11(9): uhae195. |
| [51] | Yuan K, Zhang H, Yu C, Luo N, Yan J, Zheng S, Wang B. Low phosphorus promotes NSP1-NSP2 heterodimerization to enhance strigolactone biosynthesis and regulate shoot and root architecture in rice[J]. Molecular Plant, 2023, 16(11): 1811-1831. |
/
| 〈 |
|
〉 |