利用QTL-Seq结合分子标记定位粳稻垩白粒率控制位点qChalk8
收稿日期: 2023-12-13
修回日期: 2024-03-18
网络出版日期: 2024-11-15
基金资助
河南省自然科学基金资助项目(232300421156);河南现代农业水稻产业技术体系项目(HARS-22-03-G3)
Mapping of qChalk8 Controlling Chalky Rice Rate in japonica Rice by Combining QTL-Seq with Molecular Markers
Received date: 2023-12-13
Revised date: 2024-03-18
Online published: 2024-11-15
【目的】垩白是影响稻米外观品质的重要性状。本研究旨在利用QTL-Seq和分子标记作图定位粳稻垩白粒率调控相关的QTL。【方法】利用高垩白粒率粳稻材料拉木加和低垩白粒率粳稻品种水晶3号构建F2分离群体，将两份独立的F2群体分别种植于河南原阳和海南三亚，两个群体单株垩白粒率考种后分别选取极端个体混池进行QTL-Seq分析，然后使用分子标记作图对稳定的QTL进行验证。【结果】两个F2群体的QTL-Seq分析发现8号染色体存在一个较为稳定的QTL位点qChalk8。进一步通过分子标记作图将该QTL定位于17.6－18.5 Mb，该QTL位点的LOD值为7.08，表型贡献率为15.9%。【结论】利用QTL-Seq和分子标记作图定位了粳稻垩白粒率相关QTL-qChalk8，为粳稻垩白调控基因进一步的精细定位和基因克隆奠定了基础。
杜彦修, 孙文玉, 袁泽科, 张倩倩, 李富豪, 李俊周, 孙红正 . 利用QTL-Seq结合分子标记定位粳稻垩白粒率控制位点qChalk8[J]. 中国水稻科学, 2024 , 38(6) : 665 -671 . DOI: 10.16819/j.1001-7216.2024.231207
【Objective】Chalkiness is an important characteristic that affects the appearance quality of rice grains. This study aims to use QTL-Seq and molecular marker mapping methods to locate QTLs related to chalky rice rates in japonica rice.【Method】F2 segregating populations were constructed using the japonica germplasm Lamujia with a high chalky rice rate and the japonica variety Shuijing 3 with a low chalky rice rate. Two independent F2 populations were planted in Yuanyang, Henan Province, and Sanya, Hainan Province, respectively. The single-plant chalky rice rate was examined, and extreme individuals were pooled for QTL-Seq analysis. Reliable QTLs were then subjected to verification using molecular marker mapping.【Result】QTL-Seq analysis of the two F2 populations revealed the existence of a stable chalky rice rate QTL on chromosome 8, designated as qChalk8. Further molecular marker mapping confined the QTL to a genomic region between 17.6 Mb and 18.5 Mb. The LOD score for qChalk8 was 7.08, and qChalk8 could explain 15.9% of the phenotypic variation.【Conclusion】QTL-Seq and molecular marker mapping successfully identified qChalk8 related to chalky rice rates in japonica rice. The localization of qChalk8 establishes a foundation for further fine mapping and gene cloning of the chalkiness regulatory gene in japonica rice.
Key words： rice; grain chalkiness; chalky rice rate; QTL-Seq; QTL mapping
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