钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制
收稿日期: 2022-06-08
修回日期: 2022-08-31
网络出版日期: 2023-03-10
基金资助
国家自然科学基金青年项目(31901452);国家自然科学基金青年项目(42207415);国家自然科学基金青年项目(32201702);国家自然科学基金面上项目(31872857);国家自然科学基金面上项目(31771733);国家重点研发计划课题(2022YFD1500403);国家重点研发计划课题(2022YFD2301404-2);水稻生物学国家重点实验室开放课题资助项目(20190402)
Mechanism of Interaction Between Calcium Ion and Hydrogen Sulfide Alleviating the Inhibitory Effect of Aluminum on Root Elongation in Rice
Received date: 2022-06-08
Revised date: 2022-08-31
Online published: 2023-03-10
【目的】阐明钙离子与硫化氢相互作用缓解水稻铝毒害的分子和生理机制。【方法】以Kasalath为试验材料，选取0 μmol/L和30 μmol/L AlCl3，0.1 mmol/L 和 0.5 mmol/L CaCl2，0.2 μmol/L NaHS和100 μmol/L硫化氢清除剂亚牛磺酸(HP)作为处理浓度，将种子置于30℃培养箱中黑暗培养24 h后取水稻根系，通过测定水稻根系伸长量、总铝含量、细胞汁液中铝含量、质外体中铝含量、细胞壁中铝含量、果胶含量、果胶甲酯酶活性以及OsSTAR2、OsNRAT1和OsFRDL4相对表达量，探究钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制。【结果】铝胁迫下，相较于0.1 mmol/L CaCl2处理，0.5 mmol/L CaCl2处理显著提高了水稻根系伸长量、硫化氢含量、总钙含量和细胞质中钙含量，显著降低了水稻根系的总铝含量，细胞液、质外体和细胞壁中的铝含量。铝胁迫下，硫氢化钠预处理后，水稻根系的伸长量在两种钙浓度下均显著增加，水稻的根尖铝含量、根系总铝含量、细胞液中铝含量、质外体中铝含量和细胞壁中铝含量在两种钙浓度下均显著降低，OsSTAR2和OsFRDL4相对表达量在两种钙浓度下均显著提高，OsNRAT1相对表达量在两种钙浓度下均显著降低。铝胁迫下，添加HP则呈现相反的结果。【结论】铝胁迫下，钙离子通过增加水稻根系硫化氢的生成，降低水稻根系对铝的吸收和积累，最终缓解铝对水稻根系伸长的抑制作用。
魏倩倩, 徐青山, 潘林, 孔亚丽, 朱练峰, 曹小闯, 田文昊, 刘佳, 金千瑜, 项兴佳, 张均华, 朱春权 . 钙离子与硫化氢互作缓解铝对水稻根系伸长抑制作用的机制[J]. 中国水稻科学, 2023 , 37(2) : 142 -152 . DOI: 10.16819/j.1001-7216.2023.220603
【Objective】 The objective of this study is to elucidate the molecular and physiological mechanisms of the interaction between calcium ion (Ca2+) and hydrogen sulfide (H2S) alleviating aluminum (Al) toxicity in rice.【Method】 The rice cultivar kasalath was selected as test material, and treated with 0 μmol/L and 30 μmol/L AlCl3, 0.1 mmol/L and 0.5 mmol/L CaCl2, 0.2 μmol/L NaHS and 100 μmol/L H2S scavenger hypotaurine (HP). The seeds were incubated at 30℃ in the dark for 24 h, then the root elongation, total Al content, Al content in cell sap, Al content in apoplast, Al content in cell wall, pectin content, pectin methylesterase activity and the relative expression of OsSTAR2, OsNRAT1 and OsFRDL4 were measured to explore the molecular and physiological mechanism of the interaction between Ca2+ and H2S alleviating Al toxicity in rice. 【Result】 Under Al stress, compared with 0.1 mmol/L CaCl2 treatment, 0.5 mmol/L CaCl2 treatment significantly increased root elongation, H2S content, total calcium (Ca) content and cytoplasm Ca content, and significantly decreased total Al content in root, cell sap Al content, apoplastic Al content and cell wall Al content in rice. The pretreatment of NaHS significantly increased the elongation of rice roots at the two calcium concentrations, accompanied with the significant increase of root tip Al content, root total Al content, cell sap Al content, apoplastic Al content and cell wall Al content. The relative expression levels of OsSTAR2 and OsFRDL4 were significantly increased and the relative expression of OsNRAT1 was significantly decreased at the two Ca2+ concentrations after pretreated with NaHS. However, the application of HP produced an opposite result. 【Conclusion】 Under Al stress, Ca2+ reduced the accumulation of Al in rice root cell wall and the absorption of Al by rice roots through increasing the production of H2S in rice root, and finally alleviated the inhibition of Al on rice root elongation.
Key words： rice; aluminum stress; calcium ion; hydrogen sulfide
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