水稻光温敏雄性不育突变体tms3650的鉴定和基因定位
收稿日期: 2022-01-09
修回日期: 2022-03-22
网络出版日期: 2023-01-10
基金资助
中国水稻所水稻生物学国家重点实验室开放课题资助项目(20190203)
Identification and Gene Mapping of a New Photo-thermo-sensitive Male Sterile Mutant tms3650 in Rice
Received date: 2022-01-09
Revised date: 2022-03-22
Online published: 2023-01-10
唐杰, 龙湍, 吴春瑜, 李新鹏, 曾翔, 吴永忠, 黄培劲 . 水稻光温敏雄性不育突变体tms3650的鉴定和基因定位[J]. 中国水稻科学, 2023 , 37(1) : 45 -54 . DOI: 10.16819/j.1001-7216.2023.220109
【Objective】 Male sterility is the basis of rice heterosis utilization. To further analyze the regulation mechanism, a male sterility mutant was identified.【Method】 The tms3650 mutant was isolated from the mutant library of 60Co-γ-treated indica cultivar 93-11. The indica rice Minghui 63 was crossed with the mutant tms3650 as the male parent to construct F2 and F3 populations, and the target gene was fine-mapped by map-based cloning. 【Result】 The other agronomic traits of tms3650 were the same as those of the wild type, but the anthers of the mutant were white-green and thin, the pollen could not be stained blue-black by 1% iodine-potassium iodide solution with panicle-enclosure and delayed heading stage. Genetic analysis indicated that the mutant phenotype was controlled by a pair of recessive nuclear genes. The results of fine mapping showed that the gene was located within a distance of 135.25 kb between the SSR markers RM15927 and RM15934 on the long arm of chromosome 3, and co-segregated with the RM15931 marker. The identification of southern breeding in Lingshui in winter revealed that the fertility of mutant was affected by light and temperature, indicating that the tms3650 was a photo-thermo-sensitive male sterile mutant. 【Conclusion】 By comparing with the reported male sterility genes, the tms3650 was controlled by a new gene locus, TMS3650.
Key words： rice; photo-thermo-sensitive male sterility; gene mapping
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