水稻苗期耐热种质资源筛选及QTL定位
收稿日期: 2021-07-29
修回日期: 2021-08-19
网络出版日期: 2022-05-11
基金资助
国家重点研发计划资助项目(2016YFD0100101);国家重点研发计划资助项目(2016YFD0100101-11);江西现代农业科研协同创新专项(JXXTCX202101);国家农作物种质资源保护项目(2019NWB036-01);国家农作物种质资源保护项目(2019NWB036-12-2);国家农作物种质资源平台资助项目(NCGRC2020-02);国家农作物种质资源平台资助项目(NCGR2021-055)
Screening and QTL Mapping of Heat-tolerant Rice (Oryza sativa L.) Germplasm Resources at Seedling Stage
Received date: 2021-07-29
Revised date: 2021-08-19
Online published: 2022-05-11
【目的】鉴定和筛选水稻极端耐热种质或基因，为培育耐高温水稻新品种提供技术支撑。【方法】以耐热等级和幼苗存活率为指标对不同类型水稻苗期耐热性进行鉴定评价，以筛选和鉴定耐热种质资源及主效QTL。【结果】不同类型水稻品种苗期耐热性存在明显差异，籼稻品种耐热性明显强于粳稻品种，籼稻和粳稻品种均存在极端耐热和极端敏感种质资源;共筛选出嘉育253、中优早8号、秀水09等20份耐热种质资源，高温处理后幼苗存活率和生长发育基本不受影响。RIL群体双亲耐热等级和幼苗存活率存在极显著差异，中优早8号耐热性较强，植株基本无枯死，龙稻5号对高温胁迫较敏感，不同株系间苗期耐热性存在较大幅度变异;共检测到12个苗期耐热相关QTL，分布于第1、3、4、5和8染色体上，耐热等级和存活率QTL存在明显的遗传重叠，主效QTL簇qHTS4和qHTS8表型贡献率较大。基于QTL初步定位结果，利用相对剩余杂合体RHL-F2群体，在第8染色体RM5808-RM556标记区域鉴定了一个苗期耐热性主效QTL qHTS8，该区域对苗期耐热性具有较强调控效应。【结论】筛选出20份苗期耐热性较强的水稻种质资源，鉴定了12个苗期耐热相关的QTL，定位和验证了一个调控水稻苗期耐热性的主效QTL qHTS8，研究结果可为水稻苗期耐热性生理生化机理与分子遗传机制的研究及育种利用奠定基础。
刘进, 崔迪, 余丽琴, 张立娜, 周慧颖, 马小定, 胡佳晓, 韩冰, 韩龙植, 黎毛毛 . 水稻苗期耐热种质资源筛选及QTL定位[J]. 中国水稻科学, 2022 , 36(3) : 259 -268 . DOI: 10.16819/j.1001-7216.2022
【Objective】Identification of germplasm resources and genes extremely resistant to heat will provide technical support and lay a physical foundation for the breeding of new heat-tolerant rice varieties. 【Method】Heat tolerance level and survival rate are used to identify the high temperature resistance of rice at seedling stage, so as to select heat-tolerant germplasm resources and identify major QTLs. 【Results】There are obvious differences in heat tolerance among different varieties at seedling stage. Indica rice has significantly stronger tolerance to heat than japonica rice. Twenty accessions of rice germplasm resources with strong heat tolerance, such as Jiayu 253, Zhongyouzao 8, and Xiushui 09, were selected. The survival rates, growth and development of these varieties were almost immune to high temperature. Furthermore, there are significant differences in heat tolerance between the parents. Zhongyouzao 8 has strong heat resistance, while Longdao 5 is sensitive to high temperature. The phenotype followed an approximate continuous normal distribution under high temperature in the RIL populations. Twelve QTLs were detected on chromosomes 1, 3, 4, 5 and 8, and these QTLs have a significant genetic overlap for heat resistance grade(HTG) and survival rate under heat stress(HTSR). The major QTL clusters qHTS4 and qHTS8 have significant heat tolerance function. Based on the preliminary QTL mapping results, the RHL-F2 population was used to validate the stably expressed major QTL qHTS8, which was identified on the chromosome 8 between RM5808 and RM556 regions. 【Conclusion】 Twenty accessions of rice germplasm resources with strong heat tolerance were selected, twelve QTLs were identified, and then the major QTL qHTS8 was verified to have strong effects on regulating heat tolerance. These results will lay a foundation and provide abundant germplasm resources for the research on the physiological, biochemical, and molecular genetic mechanism of heat resistance and rice breeding.
Key words： rice; seedling stage; heat tolerance; germplasm screening; QTL
| [1] | Zhang G H, Li S Y, Wang L, Ye W J, Zeng D L, Rao Y C, Peng Y L, Hu J, Yang Y L, Xu J, Ren D Y, Gao Z Y, Zhu L, Dong G J, Hu X M, Yan M X, Guo L B, Li C Y, Qian Q. LSCHL4 from japonica cultivar, which is allelic to NAL1, increases yield of indica super rice 93-11[J]. Molecular Plant, 2014, 7: 1350-1364. |
| [2] | 田小海, 罗海伟, 周恒多, 吴晨阳. 中国水稻热害研究历史、进展与展望[J]. 中国农学通报, 2009, 25(22): 166-168. |
| [2] | Tian X H, Lu H W, Zhou H D, Wu C Y. Research on heat stress of rice in China: Progress and prospect[J]. Chinese Agricultural Science Bulletin, 2009, 25(22): 166-168. (in Chinese with English abstract) |
| [3] | 杨沈斌, 申双和, 赵小艳, 赵艳霞, 许吟隆, 王主玉, 刘娟, 张玮玮. 气候变化对长江中下游稻区水稻产量的影响[J]. 作物学报, 2010, 36(9): 1519-1528. |
| [3] | Yang S B, Shen S H, Zhao X Y, Zhao Y X, Xu Y L, Wang Z Y, Liu J, Zhang W W. Impacts of climate changes on rice production in the middle and lower reaches of the Yangtze River[J]. Acta Agronomica Sinica, 2010, 36(9): 1519-1528. (in Chinese with English abstract) |
| [4] | 方芳, 何序晨, 张志豪, 张勤, 关亚静, 胡晋, 胡伟民. 玉米自交系苗期对高温胁迫的响应机制及其抗逆性[J]. 浙江农业学报, 2019, 31(7): 1045-1056. |
| [4] | Fang F, He X C, Zhang Z H, Zhang Q, Guan Y J, Hu J, Hu W M. Response mechanism and stress resistance of maize inbred lines to high temperature stress at seedling stage[J]. Acta Agriculturae Zhejiangensis, 2019, 31(7): 1045-1056. (in Chinese with English abstract) |
| [5] | Longmei N, Gill G K, Zaidi P H, Kumar R, Vikal Y. Genome wide association mapping for heat tolerance in sub-tropical maize[J]. BMC Genomics, 2021, 22: 154. |
| [6] | Das S, Krishnan P, Nayak M, Ramakrishnan B. High temperature stress effects on pollens of rice (Oryza sativa L.) genotypes[J]. Environmental and Experimental Botany, 2014, 101: 36-46. |
| [7] | Zhang X J, Zhang S Y, Li C Q, Zhang W H. Effects of high temperature stress on growth of stress-tolerant rice seedlings with resistibility[J]. Agricultural Science & Technology, 2014, 15(4): 576-578, 584. |
| [8] | Peng S B, Huang J L, Sheehy J E, Laza R C, Visperas R M, Zhong X, Centeno G S, Khush G S, Cassman K G. Rice yields decline with higher night temperature from global warming[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(27): 9971-9975. |
| [9] | Zhao C, Liu B, Piao S L, Wang X H, Lobell D B, Huang Y, Huang M T, YaoY T, Bassu S, Ciais P, Durand J L, Elliott J, Ewert F, Janssens I A, Li T, Lin E, Liu Q, Martre P, Müller C, Peng S S, Peñuelas J, Ruane A C, Wallach D, Wang T, Wu D H, Liu Z, Zhu Y, Zhu Z C, Asseng S. Temperature increase reduces global yields of major crops in four independent estimates[J]. Proceedings of the National Academy of Sciences of the United States of America, 2017, 114: 9326-9331. |
| [10] | IPCC. Climate change 2013: The physical science basis contribution of working group I to the fifth assessment report of the intergovernmental panel on climate change[R]. Cambridge: Cambridge University Press, 2013: 1-36. |
| [11] | 朱镇, 赵庆勇, 张亚东, 陈涛, 姚姝, 周丽慧, 于新, 王才林. 抽穗扬花期极端自然高温胁迫对水稻结实率的影响研究[J]. 西南农业学报, 2015, 28(1): 1666-1671. |
| [11] | Zhu Z, Zhao Q Y, Zhang Y D, Chen T, Yao S, Zhou L H, Yu X, Wang C L. Effect of extreme natural high temperature at heading and flowering stage on seed setting rate of rice (Oryza sativa L.)[J]. Southwest China Journal of Agricultural Sciences, 2015, 28(1): 1666-1671. (in Chinese with English abstract) |
| [12] | 李小湘, 姚奕, 潘孝武, 黎用朝, 刘文强, 刘利成, 盛新年, 康旭梅, 段永红. 地方稻资源D43的开花期耐热特性研究[J]. 植物遗传资源学报, 2017, 18(2): 275-282. |
| [12] | Li X X, Yao Y, Pan X W, Li Y C, Liu W Q, Liu L C, Shen X N, Kang X M, Duan Y H. Heat resistance analysis of an early-morning-flowering landrace D43 at anthesis[J]. Journal of Plant Genetic Resources, 2017, 18(2): 275-282. (in Chinese with English abstract) |
| [13] | 刘业涛, 穆麒麟, 王毅, 高园, 田小海. 从非洲水稻材料中筛选耐高温种质资源[J]. 中国农学通报, 2019, 35(12): 8-12. |
| [13] | Liu Y T, Mu Q L, Wang Y, Gao Y, Tian X H. Selecting high temperature tolerant germplasms from Africa rice[J]. Chinese Agricultural Science Bulletin, 2019, 35(12): 8-12. (in Chinese with English abstract) |
| [14] | 黎毛毛, 余丽琴, 熊玉珍, 杜慧, 吴锦文, 李慧, 张晓宁. 抽穗扬花期耐热水稻种质资源的筛选鉴定[J]. 江西农业学报, 2016, 28(6): 1-5. |
| [14] | Li M M, Yu L Q, Xiong Y Z, Du H, Wu J W, Li H, Zhang X N. Selection and identification of rice germplasm resources tolerant to high temperature at heading-flowering stage[J]. Acta Agriculturae Jiangxi, 2016, 28(6): 1-5. (in Chinese with English abstract) |
| [15] | 刘刚, 蔡海亚, 贾海涛, 张硕, 焦春海. 非洲水稻种质SDWG005苗期耐热生理基础[J]. 植物遗传资源学报, 2021, 22(3): 646-653 |
| [15] | Liu G, Cai H Y, Jia H T, Zhang S, Jiao C H. Physiological basis of heat tolerance of African rice germplasm SDWG005 at seedling stage[J]. Journal of Plant Genetic Resources, 2021, 22(3): 646-653. (in Chinese with English abstract) |
| [16] | 方先文, 汤陵华, 王艳平. 水稻孕穗期耐热种质资源的初步筛选[J]. 植物遗传资源学报, 2006, 7(3): 342-344. |
| [16] | Fang X W, Tang L H, Wang Y P. Selection on rice germplasm tolerant to high temperature[J]. Journal of Plant Genetic Resources, 2006, 7(3): 342-344. (in Chinese with English abstract) |
| [17] | 黎毛毛, 廖家槐, 张晓宁, 马小定, 杜慧, 韩龙植. 江西省早稻品种抽穗扬花期耐热性鉴定评价研究[J]. 植物遗传资源学报, 2014, 15(5): 919-925. |
| [17] | Li M M, Liao J H, Zhang X N, Ma X D, Du H, Han L Z. Evaluation of heat tolerance at the heading-flowering stages for early-season rice varieties in Jiangxi Province[J]. Journal of Plant Genetic Resources, 2014, 15(5): 919-925. (in Chinese with English abstract) |
| [18] | 杨军, 章毅之, 贺浩华, 李迎春, 陈小荣, 边建民, 金国花, 李翔翔, 黄淑娥. 水稻高温热害的研究现状与进展[J]. 应用生态学报, 2020, 31(8): 2817-2830. |
| [18] | Yang J, Zhang Y Z, He H H, Li Y C, Chen X R, Bian J M, Jin G H, Li X X, Huang S E. Current status and research advances of high-temperature hazards in rice. Chinese Journal of Applied Ecology, 2020, 31(8): 2817-2830. (in Chinese with English abstract) |
| [19] | 王志刚, 王磊, 林海, 庞乾林, 鄂志国, 张玉屏, 朱德峰. 水稻高温热害及耐热性研究进展[J]. 中国稻米, 2013, 19(1): 27-31. |
| [19] | Wang Z G, Wang L, Lin H, Pan Q L, E Z G, Zhang Y P, Zhu D F. Research progress on high temperature hot damages of rice[J]. China Rice, 2013, 19(1): 27-31. (in Chinese with English abstract) |
| [20] | 唐婷, 陈艳艳, 杨洋, 杨远柱, 孟桂元, 周静. 水稻耐高温性研究进展[J]. 分子植物育种, 2017, 15(9): 3694-3700. |
| [20] | Tang T, Cheng Y Y, Yang Y, Yang Y Z, Meng G Y, Zhou J. Research progress of rice resistance to high temperature[J]. Molecular Plant Breeding, 2017, 15(9): 3694-3700. (in Chinese with English abstract) |
| [21] | Liu Y L, Liu X Y, Wang X, Gao K, Zheng S. Heterologous expression of heat stress responsive AtPLC9 confers heat tolerance in transgenic rice[J]. BMC Plant Biology, 2020, 20: 514. |
| [22] | 穰中文, 周清明. 水稻高温胁迫的生理响应及耐热机理研究进展[J]. 中国农学通报, 2015, 31(21): 249-258. |
| [22] | Xiang Z W, Zhou Q M. Research advances on physiological responses and tolerant mechanism to high temperature stress in rice[J]. Chinese Agricultural Science Bulletin, 2015, 31(21): 249-258. (in Chinese with English abstract) |
| [23] | 万丙良, 查中萍. 气候变暖对水稻生产的影响及水稻耐高温遗传改良[J]. 中国农学通报, 2012, 28(36): 1-7. |
| [23] | Wan B L, Zha Z P. Effects of climate warming on rice production and genetic improvement for rice heat- tolerance[J]. Chinese Agricultural Science Bulletin, 2012, 28(36): 1-7. (in Chinese with English abstract) |
| [24] | 凌霄霞, 张作林, 翟景秋, 叶树春, 黄见良. 气候变化对中国水稻生产的影响研究进展[J]. 作物学报, 2019, 45(3): 323-334. |
| [24] | Ling X X, Zhang Z L, Zhai J Q, Ye S C, Huang J L. A review for impacts of climate change on rice production in China[J]. Acta Agronomica Sinica, 2019, 45(3): 323-334. (in Chinese with English abstract) |
| [25] | 刘进, 姚晓云, 刘丹, 余丽琴, 李慧, 王棋, 王嘉宇, 黎毛毛. 不同生态环境下水稻穗部性状QTL鉴定[J]. 中国水稻科学, 2019, 33(2): 124-134. |
| [25] | Liu J, Yao X Y, Liu D, Yu L Q, Li H, Wang Q, Wang J Y, Li M M. Identification of QTL for panicle traits under multiple environments in rice(Oryza sativa L.)[J]. Chinese Journal of Rice Science, 2019, 33(2): 124-134. (in Chinese with English abstract) |
| [26] | Wang J K, Li H H, Zhang L Y, Meng L. QTL IciMapping Version 4.2[OE/OL]. (2019-07-24) [2021-05-05]. http://www.isbreeding.net. |
| [27] | McCouch S R. Gene nomenclature system for rice[J]. Rice, 2008, 1: 72-84. |
| [28] | 熊伟, 冯灵芝, 居辉, 杨笛. 未来气候变化背景下高温热害对中国水稻产量的可能影响分析[J]. 地球科学进展, 2016, 31(5): 515-528. |
| [28] | Xiong W, Feng L Z, Ju H, Yang D. Possible impacts of high temperatures on china's rice yield under climate change[J]. Advances in Earth Science, 2016, 31(5): 515-528. (in Chinese with English abstract) |
| [29] | 冯活仪, 江浩林, 王孟, 唐湘如, 段美洋, 潘圣刚, 田华, 王树丽, 莫钊文. 不同香稻品种苗期耐高温的形态生理响应[J]. 中国水稻科学, 2019, 33(1): 68-74. |
| [29] | Feng H Y, Jiang H L, Wang M, Tang X R, Duan M Y, Pan S G, Tian H, Wang S L, Mo Z W. Morpho- physiological responses of different scented rice varieties to high temperature at seedling stage[J]. Chinese Journal of Rice Science, 2019, 33(1): 68-74. (in Chinese with English abstract) |
| [30] | 段骅, 杨建昌. 高温对水稻的影响及其机制的研究进展[J]. 中国水稻科学, 2012, 26(4): 393-400. |
| [30] | Du H, Yang J C. Research advances in the effect of high temperature on rice and its mechanism[J]. Chinese Journal of Rice Science, 2012, 26(4): 393-400. (in Chinese with English abstract) |
| [31] | 许红云, 许为军, 谭学林. 高温对粳稻品种发芽及幼苗生长发育影响的研究[J]. 西南农业学报, 2008, 21(3): 593-596. |
| [31] | Xu H Y, Xu W J, Tan X L. Effect of continuing high temperature on germination and seedling development of japonica rice[J]. Southwest China Journal of Agricultural Sciences, 2008, 21(3): 593-596. (in Chinese with English abstract) |
| [32] | 何洋, 刘洋, 方宝华, 何小娥, 杨坚, 滕振宁, 张玉烛. 不同生育期温度逆境处理对早稻产量的影响[J]. 中国农学通报, 2016, 32(24): 18-24 |
| [32] | He Y, Liu Y, Fang B H, He X E, Yang J, Teng Z N, Zhang Y Z. Effect of temperature on early rice yield at different growth stages[J]. Chinese Agricultural Science Bulletin, 2016, 32(24): 18-24. (in Chinese with English abstract) |
| [33] | 赵凌, 赵春芳, 王建, 陈涛, 赵庆勇, 姚姝, 王才林. 20个水稻引进资源的花药开裂及苗期耐热性评价[J]. 植物遗传资源学报, 2017, 18(5): 968-973. |
| [33] | Zhao L, Zhao C F, Wang J, Chen T, Zhao Q Y, Yao S, Wang C L. Analyzing of length dehiscence at basal part of thecae and heat resistance during seedling stage of 20 introduced rice germplasms[J]. Journal of Plant Genetic Resources, 2017, 18(5): 968-973. (in Chinese with English abstract) |
| [34] | Yan C, Zhan GP, Hong X F, Yang D W. Identification and fine mapping of a major QTL, TT1-2, that plays significant roles in regulating heat tolerance in rice[J]. Plant Molecular Biology Reporter, 2021, 39: 376-385. |
| [35] | 刘鸣, 李玉花, 解莉楠. 水稻非生物胁迫相关QTL研究进展[J]. 植物生理学报, 2013, 49(12): 1301-1308. |
| [35] | Liu M, Li Y H, Xie L N. Progress in QTL research for abiotic stress in rice[J]. Plant Physiology Journal, 2013, 49(12): 1301-1308. (in Chinese with English abstract) |
| [36] | 王胜昌, 涂海甫, 胡丹, 吴奈, 岑祥, 熊立仲. 水稻抗非生物逆境功能基因的发掘[J]. 生命科学, 2016, 28(10): 1216-1229. |
| [36] | Wang S C, Tu H F, Hu D, Wu N, Qin X, Xiong L Z. The exploitation of rice functional genes for abiotic stress[J]. Chinese Bulletin of Life Sciences, 2016, 28(10): 1216-1229. (in Chinese with English abstract) |
| [37] | Wada H,. Hirata R, Matsuda R. New approaches combined with environmental control for enhancing heat-tolerant rice breeding in Japan//Iizumi T, Adaptation to Climate Change in Agriculture[M]. Singapore: Springer, 2019: 37-51. |
| [38] | Li G Y, Zhang C X, Zhang G H, Fu W M, Feng B H, Chen T T, Peng S B, Tao L X, Fu G F. Abscisic acid negatively modulates heat tolerance in rolled leaf rice by increasing leaf temperature and regulating energy homeostasis[J]. Rice, 2020, 13: 18. |
| [39] | Jagadish S V K, Cairns J, Lafitte R, Wheeler T R, Price A H, Craufurd P Q. Genetic analysis of heat tolerance at anthesis in rice[J]. Crop Science, 2010, 50(5): 1633-1641. |
| [40] | 潘孝武, 李小湘, 黎用朝, 姚奕, 刘文强, 盛新年. 水稻生殖发育期耐热性的分子遗传机制研究进展[J]. 生物技术通报, 2015, 31(4): 40-46. |
| [40] | Pan X W, Li X X, Li Y C, Yao Y, Liu W Q, Shen X N. Advances on molecular and genetic mechanisms of rice heat tolerance at the reproductive stage[J]. Biotechnology Bulletin, 2015, 31(4): 40-46. (in Chinese with English abstract) |
| [41] | Raza Q, Riaz A, Bashir K, Sabar M. Reproductive tissues specific meta-QTLs and candidate genes for development of heat tolerant rice cultivars[J]. Plant Molecular Biology, 2020, 104: 97-112. |
| [42] | Li X M, Chao D Y, Wu Y, Huang X H, Chen K, Cui L G, Su L, Ye W W, Chen H, Chen H C, Dong N Q, Guo T, Shi M, Feng Q, Zhang P, Han B, Shan X J, Gao J P, Lin H X. Natural alleles of a proteasome α2 subunit gene contribute to thermos-tolerance and adaptation of African rice[J]. Nature Genetics, 2015, 47: 827-833. |
| [43] | Liu J, Zhang C, Wei C, Liu J P, Zhan C C, Wei C C, Liu X, Wang M G, Yu F F, Xie Q, Tu J M. The RING finger ubiquitin E3 ligase OsHTAS enhances heat tolerance by promoting H2O2-induced stomatal closure in rice[J]. Plant Physiology, 2016, 170: 429-443. |
| [44] | Wang D, Qin B X, Li X, Tang D, Zhang Y E, Cheng Z K, Xue Y B. Nucleolar DEAD-Box RNA helicase TOGR1 regulates thermos-tolerant growth as a Pre-rRNA chaperone in rice[J]. PLOS Genetics, 2016, 12(2): e1005844. |
| [45] | Ye C, Tenorio F A, Redona E D, Cortezano P S M, Cabrega G A, Jagadish K S V, Gregorio G B. Fine-mapping and validating qHTSF4.1 to increase spikelet fertility under heat stress at flowering in rice[J]. Theoretical and Applied Genetics, 2015, 128: 1507-1517. |
| [46] | Chen L, Wang Q, Tang M Y, Zhang X L, Pan Y H, Yang X H, Gao G Q, Lü R H, Tao W, Jiang LG, Liang T F. QTL mapping and identification of candidate genes for heat tolerance at the flowering stage in rice[J]. Frontiers in Genetics, 2021, 11: 621871. |
| [47] | 朱玉君, 左紫薇, 张振华, 樊叶杨. 一种水稻微效QTL精细定位和克隆新途径[J]. 中国水稻科学, 2021, 35(4): 407-414. |
| [47] | Zhu Y J, Zuo Z W, Zhang Z H, Fan Y Y. A new approach for fine-mapping and map-based cloning of minor-effect QTL in rice[J]. Chinese Journal of Rice Science, 2021, 35(4): 407-414. (in Chinese with English abstract) |
| [48] | 杜成兴, 张华丽, 戴冬青, 吴明月, 梁敏敏, 陈俊宇, 马良勇. 水稻粒重粒形QTL 的定位及qTGW1.2/ qGL1.2的验证[J]. 中国水稻科学, 2021, 35(4): 359-372. |
| [48] | Du C X, Zhang H L, Dai D Q, Wu M Y, Liang M M, Chen J Y, Ma L Y. QTL analysis for grain weight and shape and validation of qTGW1.2/qGL1.2[J]. Chinese Journal of Rice Science, 2021, 35(4): 359-372. (in Chinese with English abstract) |
/
| 〈 |
|
〉 |