氯虫苯甲酰胺胁迫下二化螟中肠细菌类微生物的多样性
收稿日期: 2020-01-06
修回日期: 2020-04-20
网络出版日期: 2020-11-10
基金资助
浙江省公益科技计划资助项目 (2016C32100);现代农业产业技术体系专项(CARS-24-G-06)
Diversity of Midgut Microbial Community of ChilosuppressalisExposed to Chlorobenzamide
Received date: 2020-01-06
Revised date: 2020-04-20
Online published: 2020-11-10
关键词： 二化螟; 氯虫苯甲酰胺：宏基因组; 中肠细菌; 微生物多样性
张珏锋, 张琴, 李芳, 钟海英, 陈建明 . 氯虫苯甲酰胺胁迫下二化螟中肠细菌类微生物的多样性[J]. 中国水稻科学, 2020 , 34(6) : 586 -594 . DOI: 10.16819/j.1001-7216.2020.0101
【Objective】We aim toilluminatethestructural changes in the midgut microbial community of Chilosuppressalis exposed to chlorobenzamide.【Method】Macrogenomic sequencing and in vitro culturewere used to analyze the diversity of midgut microbiotaof Chilosuppressalistreatedwith different concentrations (100, 200, 400µg/mL) of chlorobenzamide.【Result】The results showed that chlorobenzamide treatmentdecreased the abundance anddiversityof the midgut bacteria of Chilosuppressalis,and the OTU number and specific OTU number of the intestinal bacteria of treated population were lower than those of the control;the proportion of Morganella, Provincia and Proteus in the chlorobenzamide treated population increased significantly. Meanwhile, the result of in vitro culture indicated besides enterobacter,Lelliottinimipressuralis, Leclerciadecarboxylata, Pantoeaagglomeranswere isolated from chlorobenzamide treated Chilosuppressalis population. COG classification and KEGG analysis didn’t show significant difference in number and relative abundance of functional genes among control and three chlorobenzamide treated populations.【Conclusion】The study had clarified the diversity of intestinal bacteria and the relationshipbetween gutbacteria of Chilosuppressalis andthe formation of chlorobenzamide resistance, and will lay a scientific basis for the role of intestinal bacteria in resistance of Chilosuppressalistochlorobenzamide.
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