应用东乡野生稻回交重组自交系分析水稻耐低氮产量相关性状QTL
收稿日期: 2020-04-30
修回日期: 2020-06-03
网络出版日期: 2020-11-10
基金资助
江西省自然科学基金资助项目(20171ACB21071);国家自然科学基金资助项目(31760378);农业农村部长江中下游籼稻遗传育种重点实验室开放课题资助项目(2018KLMA02);水稻生物学国家重点实验室开放课题(20200201)
QTL Analysis for Yield Traits Related to Low Nitrogen Tolerance Using Backcrossing Recombinant Inbred Lines Derived from Dongxiang Wild Rice (Oryza rufipogon Griff.)
Received date: 2020-04-30
Revised date: 2020-06-03
Online published: 2020-11-10
吴婷, 李霞, 黄得润, 黄凤林, 肖宇龙, 胡标林 . 应用东乡野生稻回交重组自交系分析水稻耐低氮产量相关性状QTL[J]. 中国水稻科学, 2020 , 34(6) : 499 -511 . DOI: 10.16819/j.1001-7216.2020.0408
【Objective】Dongxiang wildrice (Oryzarufipogon Griff.) has strong low nitrogen toleranceand is an important germplasm for low nitrogen tolerance improvement. Identification of genes responsible for low nitrogen tolerance in Dongxiang wild rice is of great importance to understand molecular mechanisms of low nitrogen tolerance and develop rice varieties with low nitrogen tolerance. 【Method】Quantitative trait loci (QTLs) for plant height and yield traits under low and normal nitrogen conditions wasidentified using backcrossing recombinant inbred lines (BC1F12) derived from an interspecific cross Xieqingzao B // Dongxiang wild rice/Xieqingzao B and its genetic linkage. 【Result】A total of 57 QTLs were detected in 33 regions on all chromosome, exceptchromosome 4 and 8. They explained individually 3.17%~63.40% phenotypic variation, and 32 QTLsof them had favorable alleles derived from Dongxiang wild rice.NineteenQTLs were simultaneously detected under both nitrogen treatments, and 38QTLs were only identified under single nitrogen treatment, suggesting variousgenetic mechanismsin rice growth and yield formation under low and normal nitrogen conditions. 【Conclusion】Fourteen QTLclusters, 43 QTLs included, scattered on sevenchromosomes, indicatingthe common genetic-physiological mechanisms behind different traits, and theQTL pyramiding for low nitrogen tolerance can be achieved by molecular marker-assisted selection.
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