江苏近年育成粳稻新品种/系的稻瘟病抗性基因及穗颈瘟抗性分析
收稿日期: 2020-02-20
修回日期: 2020-03-30
网络出版日期: 2020-09-10
基金资助
江苏省重点研发计划资助项目(BE2019339);江苏省农业重大新品种创制项目(PZCZ201703);扬州大学研究生国际学术交流专项基金资助项目(201701023)
Analysis of Blast Resistance Genes and Neck Blast Resistance of japonica Rice Varieties/Lines Recently Developed in Jiangsu Province
Received date: 2020-02-20
Revised date: 2020-03-30
Online published: 2020-09-10
【目的】明确水稻品种携带的抗稻瘟病基育种应用价值,是利用抗病基因培育抗病品种控制病害流行的重要前期工作。【方法】利用功能标记分析了14个抗稻瘟病基因在江苏近年育成的195个粳稻新品种/系中的分布情况,并对其中158个品种和17份携带抗稻瘟病基因Pigm的高世代回交株系进行穗颈瘟接种鉴定。【结果】大多数品种携带2~5个抗病基因,但所有品种均不含有Pigm基因;Pib、Pita和Pikh基因在供试品种中的分布频率较高,均在45%以上,其余基因均在30%以内;Pid3、Pid2、Pia、Pb1在新育成品种中的出现频率明显高于审定品种。测试品种对穗颈瘟的抗性主要与3个基因显著相关,贡献率由高至低依次为Pia、Pi3/5/i和Pita;其中,Pia或Pi3/5/i与Pita间的聚合效应均显著高于各基因单独存在时的抗病效应,且以Pia和Pita间的聚合效应最强,携带该基因组合的所有品种对穗颈瘟均表现抗至高抗水平抗性。回交导入Pigm基因的所有17份株系对穗颈瘟的抗性均显著高于各自轮回亲本,且均达到了抗病以上水平。【结论】抗病基因Pigm及基因组合“Pia+Pita”在江苏粳稻抗穗颈瘟育种中具有重要的育种应用价值。
王小秋, 杜海波, 陈夕军, 李明友, 王嘉楠, 许志文, 冯志明, 陈宗祥, 左示敏 . 江苏近年育成粳稻新品种/系的稻瘟病抗性基因及穗颈瘟抗性分析[J]. 中国水稻科学, 2020 , 34(5) : 413 -424 . DOI: 10.16819/j.1001-7216.2020.0208
【Objective】Evaluating the breeding potential of the known blast resistance (R) genes harbored in rice varieties is one of prerequisites for developing rice blast resistant varieties and R genes-based control of the disease epidemic. 【Method】A total of 195 new japonica varieties/lines from Jiangsu Province were genotyped using functional markers corresponding to 14 rice blast R gene loci. In order to identify gene or gene combination with breeding potential against neck-blast, 158 japonica rice varieties were then artificially inoculated with blast strains as well as 17 advanced backcrossing lines containing Pigm gene in two different genetic backgrounds at booting stage. 【Result】Most varieties carried 2 to 5 R genes, and none of new varieties contained Pigm; the distribution frequencies of Pib, Pita and Pikh were relatively high and all exceeded 45%, the rest 10 genes were under 30%; Pid3, Pid2, Pia and Pb1 had apparently higher frequency in lines from regional tests than that in authorized and released varieties. Three genes with effects ordered as Pia > Pi3/5/i > Pita significantly contributed to neck-blast resistance, and significantly positive interaction effects were found between Pia and Pita, and between Pi3/5/i and Pita. Combination effect of Pia+Pita was significantly higher than that of Pi3/5/i+Pita, and the varieties harboring Pia+Pita reached resistant or highly resistant levels. The resistance of 17 advanced backcrossing lines carrying Pigm was all significantly improved as compared with corresponding controls, and all reached resistant level or even higher. 【Conclusion】 The results suggested that Pigm and the combination of Pia+Pita have critically important breeding potential in japonica rice breeding against neck blast in Jiangsu Province.
Key words： japonica rice; neck blast; resistance gene; gene distribution; breeding value
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