优质食味粳稻辽粳433和越光糙米代谢产物差异分析
收稿日期: 2019-11-01
修回日期: 2020-01-17
网络出版日期: 2020-07-10
基金资助
国家重点研发计划资助项目(2018YFD0200200);辽宁省科学事业公益研究基金资助项目(GY-20180046)
Difference of Metabolites in Brown Rice Between Liaojing 433 and Koshihikari with Good Eating Quality
Received date: 2019-11-01
Revised date: 2020-01-17
Online published: 2020-07-10
【目的】分析优质食味粳稻辽粳433和越光之间代谢产物差异,为优质食味粳稻辽粳433推广及优质食味粳稻品种选育提供参考。【方法】利用气相色谱-质谱(GC-MS)检测辽粳433和越光的糙米代谢产物,采用在线软件对两个品种糙米差异代谢产物和差异代谢通路进行分析。【结果】通过GC-MS检测两个品种均定性到233种代谢产物。与越光相比,辽粳433糙米代谢产物相对含量高的有158种,占检测到的代谢物总数的67.81%。PCA和PLS-DA分析可以将辽粳433和越光糙米代谢产物分离开。辽粳433与越光糙米之间共有64种代谢产物含量差异显著,辽粳433糙米中相对含量高的差异代谢产物有42种,与营养相关的氨基葡萄糖酸、三糖、纤维二糖、4-氨基丁酸、油酸、α-生育酚、泛酸、肌醇、奎尼酸和绿原酸分别是越光的23.86、13.35、2.53、2.44、9.32、2.66、2.90、3.54、4.05和2.10倍;越光糙米中相对含量高的差异代谢产物有22种,与营养相关的阿拉伯糖醇、亚油酸、阿魏酸和肌醇-4-磷酸是辽粳433的1.61、2.33、1.39和2.39倍。辽粳433与越光糙米之间11个代谢通路有显著差异,肌醇磷酸代谢、抗坏血酸和醛酸代谢、酪氨酸代谢和苯丙烷生物合成检测到的代谢产物相对含量均表现为辽粳433高于越光。【结论】辽粳433与越光糙米之间代谢产物组成差异显著。与越光相比,辽粳433具有与营养相关的差异代谢产物数量更多,含量更高。辽粳433具有成为高膳食纤维和保健功能大米的潜力。GC-MS代谢组学分析方法可作为水稻品种间营养差异分析的有力工具。
沈枫, 蒋洪波, 刘博, 张秀茹, 刘军, 解文孝, 姚继攀, 马亮 . 优质食味粳稻辽粳433和越光糙米代谢产物差异分析[J]. 中国水稻科学, 2020 , 34(4) : 359 -367 . DOI: 10.16819/j.1001-7216.2020.9116
【Objective】Our goal is to analyze the metabolite difference between good eating-quality japonica rice Liaojing 433 and Koshihikari, and provide reference for breeding good eating-quality rice. 【Method】 GC-MS was used to detect metabolites in brown rice of Liaojing 433 and Koshihikari. Online software was used to analyze metabolic spectrum and metabolic pathway differences. 【Result】 233 metabolites were identified in both cultivars by GC-MS, among which 158 in Liaojing 433 have higher contents than in Koshihikari, accounting for 67.81% of the total. PCA and PLS-DA analysis found that there were different metabolic profiles between Liaojing 433 and Koshihikari with significant differences in contents of 64 metabolites. Among different metabolites, 42 metabolites had relative high contents in Liaojing 433. Glucosamine acid, trisaccharide, cellobiose, 4-aminobutyric acid, oleic acid, α-tocopherol, pantothenic acid, inositol, quinic acid and chlorogenic acid of Liaojing 433 were 23.86, 13.35, 2.53, 2.44, 9.32, 2.66, 2.90, 3.54, 4.05 and 2.10 times as many as those in Koshihikari, respectively. There are 22 kinds of relative high contents in brown rice in Koshihikari, and the nutrition-related arabinol, linoleic acid, ferulic acid and inositol-4-phosphoric acid are 1.61, 2.33, 1.39 and 2.39 times as many of those in Liaojing 433. Between Liaojing 433 and Koshihikari, there were significant differences in 11 metabolic pathways. The relative contents of metabolites detected by inositol phosphate metabolism, ascorbic acid and aldehyde acid metabolism, tyrosine metabolism and phenylpropane biosynthesis of Liaojing 433 were all higher than those of Koshihikari. 【Conclusion】 The metabolite composition of Liaojing 433 and Koshihikari brown rice was significantly different. Compared with Koshihikari, Liaojing 433 has a good taste and high dietary fiber content. In addition, Liaojing 433 has stronger health care function and antioxidant capacity. GC-MS metabolomics analysis method is a powerful tool for analyzing nutritional differences among rice varieties.
Key words： good eating quality; brown rice; metabolomics; nutritional quality
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