中国水稻科学 ›› 2020, Vol. 34 ›› Issue (4): 359-367.DOI: 10.16819/j.1001-7216.2020.9116
沈枫, 蒋洪波, 刘博, 张秀茹, 刘军, 解文孝, 姚继攀, 马亮*()
收稿日期:
2019-11-01
修回日期:
2020-01-17
出版日期:
2020-07-10
发布日期:
2020-07-10
通讯作者:
马亮
基金资助:
Feng SHEN, Hongbo JIANG, Bo LIU, Xiuru ZHANG, Jun LIU, Wenxiao XIE, Jipan YAO, Liang MA*()
Received:
2019-11-01
Revised:
2020-01-17
Online:
2020-07-10
Published:
2020-07-10
Contact:
Liang MA
摘要:
【目的】分析优质食味粳稻辽粳433和越光之间代谢产物差异,为优质食味粳稻辽粳433推广及优质食味粳稻品种选育提供参考。【方法】利用气相色谱-质谱(GC-MS)检测辽粳433和越光的糙米代谢产物,采用在线软件对两个品种糙米差异代谢产物和差异代谢通路进行分析。【结果】通过GC-MS检测两个品种均定性到233种代谢产物。与越光相比,辽粳433糙米代谢产物相对含量高的有158种,占检测到的代谢物总数的67.81%。PCA和PLS-DA分析可以将辽粳433和越光糙米代谢产物分离开。辽粳433与越光糙米之间共有64种代谢产物含量差异显著,辽粳433糙米中相对含量高的差异代谢产物有42种,与营养相关的氨基葡萄糖酸、三糖、纤维二糖、4-氨基丁酸、油酸、α-生育酚、泛酸、肌醇、奎尼酸和绿原酸分别是越光的23.86、13.35、2.53、2.44、9.32、2.66、2.90、3.54、4.05和2.10倍;越光糙米中相对含量高的差异代谢产物有22种,与营养相关的阿拉伯糖醇、亚油酸、阿魏酸和肌醇-4-磷酸是辽粳433的1.61、2.33、1.39和2.39倍。辽粳433与越光糙米之间11个代谢通路有显著差异,肌醇磷酸代谢、抗坏血酸和醛酸代谢、酪氨酸代谢和苯丙烷生物合成检测到的代谢产物相对含量均表现为辽粳433高于越光。【结论】辽粳433与越光糙米之间代谢产物组成差异显著。与越光相比,辽粳433具有与营养相关的差异代谢产物数量更多,含量更高。辽粳433具有成为高膳食纤维和保健功能大米的潜力。GC-MS代谢组学分析方法可作为水稻品种间营养差异分析的有力工具。
沈枫, 蒋洪波, 刘博, 张秀茹, 刘军, 解文孝, 姚继攀, 马亮. 优质食味粳稻辽粳433和越光糙米代谢产物差异分析[J]. 中国水稻科学, 2020, 34(4): 359-367.
Feng SHEN, Hongbo JIANG, Bo LIU, Xiuru ZHANG, Jun LIU, Wenxiao XIE, Jipan YAO, Liang MA. Difference of Metabolites in Brown Rice Between Liaojing 433 and Koshihikari with Good Eating Quality[J]. Chinese Journal OF Rice Science, 2020, 34(4): 359-367.
图1 辽粳433与越光糙米代谢产物之间PCA和PLS-DA分析 A-PCA分析,主成分1解释了总变异的22.4%,主成分2解释了总变异的15%;B-PLS-DA分析,主成分1解释了总变异的22.3%,主成分2解释了总变异的8.8%。
Fig. 1. PCA and PLS-DA of metabolites of Liaojing 433 and Koshihikari. A, The principal component 1 accounted for 22.4% of the total variation, and the principal component 2 accounted for 15% of the total variation by PCA(principal components analyses); B, The principal component 1 accounted for 22.3% of the total variation, and principal component 2 accounted for 8.8% of the total variation by PLS-DA(partial least-squares discrimination analysis).
序号 No. | 代谢产物 Metabolites | VIP | P | 序号 No. | 代谢产物 Metabolites | VIP | P |
---|---|---|---|---|---|---|---|
1 | 儿茶素 Catechin | 1.4364 | 33 | 奎宁酸 Quinic acid | 1.4048 | ||
2 | 苯乙酰胺 Phenylacetamide | 2.0567 | 34 | 4-氨基丁酸 4-aminobutyric acid | 1.1059 | ||
3 | N-乙酰-D-己糖胺 | 1.1147 | 35 | 油酸 Oleic acid | 1.0250 | ||
N-acetyl-D-hexosamine | 36 | 海藻糖-6-磷酸 Trehalose-6-phosphate | 1.1060 | ||||
4 | 正辛醛 Octanal | 3.5360 | 37 | 3-羟基-3-4'-羟基-3'-甲氧基苯丙酸 | 1.6832 | 3.7968 | |
5 | 核糖-5-磷酸 Ribose-5-phosphate | 1.2869 | 3-hydroxy-3-4'-hydroxy-3'-methoxyphe | ||||
6 | 3-羟基苯甲酸 3-hydroxybenzoic acid | 1.2889 | 3.5573 | nylpropionic acid | |||
7 | 肌醇-4-单磷酸 | 1.5187 | 5.4579 | 38 | 肌酐 Creatinine | 2.3204 | 4.9027 |
Inositol-4-monophosphate | 39 | 鸟苷 Guanosine | 1.1265 | 3.3626 | |||
8 | 甘油 Glycerol | 2.1451 | 40 | 帕拉金糖醇 Palatinitol | 1.5140 | 3.8164 | |
9 | 尿酸 Uric acid | 5.8882 | 41 | 双半乳糖醛酸 Digalacturonic acid | 3.9823 | 2.5444 | |
10 | 延胡索酸 Fumaric acid | 3.3667 | 42 | 氨基葡萄糖酸 Glucosaminic acid | 4.4534 | 4.4615 | |
11 | 半乳糖醛酸 Galacturonic acid | 3.0153 | 43 | 2'-脱氧鸟苷 2'-deoxyguanosine | 6.3652 | 9.7448 | |
12 | β-甘油磷酸 Beta-glycerolphosphate | 1.0675 | 3.3689 | 44 | 胱硫醚 Cystathionine | 2.1555 | 7.5433 |
13 | 磷酸甘油酯 Glycerol-α-phosphate | 1.2960 | 2.4937 | 45 | 1, 5-脱水葡萄糖醇 1, 5-anhydroglucitol | 2.9614 | |
14 | 阿魏酸 Ferulic acid | 3.4483 | 46 | 5-羟基-3-吲哚乙酸 | 1.3300 | 4.1545 | |
15 | 乳糖酸 Lactobionic acid | 2.5625 | 5-hydroxy-3-indoleacetic acid | ||||
16 | 1-甲基海因 1-methylhydantoin | 3.7246 | 4.2807 | 47 | 6-脱氧葡萄糖 6-Deoxyglucose | 1.0534 | 5.8862 |
17 | 赖氨酸 Lysine | 1.1595 | 3.5415 | 48 | 肌醇 Myo-inositol | 1.1812 | 6.4424 |
18 | 2-酮葡萄糖二甲基缩醛 | 1.7079 | 49 | 泛酸 Pantothenic acid | 1.5303 | 5.4521 | |
2-ketoglucose dimethylacetal | 50 | N-乙酰半乳糖胺 N-acetylgalactosamine | 4.0490 | ||||
19 | 己内酰胺 Epsilon-caprolactam | 2.6232 | 2.3184 | 51 | 四氧酸 Alloxanoic acid | 1.2308 | 3.9297 |
20 | 阿拉伯糖醇 Arabitol | 1.1096 | 52 | 绿原酸 Chlorogenic acid | 1.0311 | 3.9339 | |
21 | 胞嘧啶 Cytosin | 2.1537 | 53 | 瓜氨酸 Citrulline | 1.2555 | ||
22 | 亚油酸 Linoleic acid | 1.1261 | 54 | 鸟氨酸 Ornithine | 1.4025 | ||
23 | 琥珀酸 Succinic acid | 1.0970 | 55 | 胍丁胺 Agmatine | 2.2319 | ||
24 | 木糖 Xylulose | 1.0928 | 56 | α-生育酚 Alpha tocopherol | 2.5750 | ||
25 | 二十二碳烯酸 Docosenoic acid | 1.2319 | 57 | β-丙氨酸 Beta-alanine | 1.2123 | 2.3480 | |
26 | 1, 2, 3, 4, 5, 6, 6氘代-葡萄糖 | 1.1868 | 58 | 纤维二糖 Cellobiose | 1.4162 | 2.9658 | |
Glucose-1, 2, 3, 4, 5, 6, 6 deuterated | 59 | 核糖醇 Ribitol | 2.7145 | ||||
27 | 二十八酸 Montanic acid | 1.5310 | 60 | 2-(哌啶基)苯甲腈 | 2.3812 | ||
28 | 半乳酸 Galactonic acid | 1.1788 | 2.3643 | 2-piperidinobenzonitrile | |||
29 | 乳酸 Lactic acid | 1.1132 | 61 | 5-单磷酸胞苷 Cytidine-5-monophosphate | 3.6268 | ||
30 | 1, 2, 4-苯三酚 1, 2, 4-benzenetriol | 1.7583 | 62 | β-甘露醇甘油酯 Beta-mannosylglycerate | 3.1918 | ||
31 | 三糖 Trisaccharide | 2.3646 | 63 | 5-甲氧色胺 5-methoxytryptamine | 2.1717 | ||
32 | 肌苷 Inosine | 1.2861 | 64 | 腐胺 Putrescine | 2.1706 |
表1 辽粳433与越光糙米之间差异代谢产物种类与数量
Table 1 Different metabolic species and quantity between brown rice of Liaojing 433 and Koshihikari.
序号 No. | 代谢产物 Metabolites | VIP | P | 序号 No. | 代谢产物 Metabolites | VIP | P |
---|---|---|---|---|---|---|---|
1 | 儿茶素 Catechin | 1.4364 | 33 | 奎宁酸 Quinic acid | 1.4048 | ||
2 | 苯乙酰胺 Phenylacetamide | 2.0567 | 34 | 4-氨基丁酸 4-aminobutyric acid | 1.1059 | ||
3 | N-乙酰-D-己糖胺 | 1.1147 | 35 | 油酸 Oleic acid | 1.0250 | ||
N-acetyl-D-hexosamine | 36 | 海藻糖-6-磷酸 Trehalose-6-phosphate | 1.1060 | ||||
4 | 正辛醛 Octanal | 3.5360 | 37 | 3-羟基-3-4'-羟基-3'-甲氧基苯丙酸 | 1.6832 | 3.7968 | |
5 | 核糖-5-磷酸 Ribose-5-phosphate | 1.2869 | 3-hydroxy-3-4'-hydroxy-3'-methoxyphe | ||||
6 | 3-羟基苯甲酸 3-hydroxybenzoic acid | 1.2889 | 3.5573 | nylpropionic acid | |||
7 | 肌醇-4-单磷酸 | 1.5187 | 5.4579 | 38 | 肌酐 Creatinine | 2.3204 | 4.9027 |
Inositol-4-monophosphate | 39 | 鸟苷 Guanosine | 1.1265 | 3.3626 | |||
8 | 甘油 Glycerol | 2.1451 | 40 | 帕拉金糖醇 Palatinitol | 1.5140 | 3.8164 | |
9 | 尿酸 Uric acid | 5.8882 | 41 | 双半乳糖醛酸 Digalacturonic acid | 3.9823 | 2.5444 | |
10 | 延胡索酸 Fumaric acid | 3.3667 | 42 | 氨基葡萄糖酸 Glucosaminic acid | 4.4534 | 4.4615 | |
11 | 半乳糖醛酸 Galacturonic acid | 3.0153 | 43 | 2'-脱氧鸟苷 2'-deoxyguanosine | 6.3652 | 9.7448 | |
12 | β-甘油磷酸 Beta-glycerolphosphate | 1.0675 | 3.3689 | 44 | 胱硫醚 Cystathionine | 2.1555 | 7.5433 |
13 | 磷酸甘油酯 Glycerol-α-phosphate | 1.2960 | 2.4937 | 45 | 1, 5-脱水葡萄糖醇 1, 5-anhydroglucitol | 2.9614 | |
14 | 阿魏酸 Ferulic acid | 3.4483 | 46 | 5-羟基-3-吲哚乙酸 | 1.3300 | 4.1545 | |
15 | 乳糖酸 Lactobionic acid | 2.5625 | 5-hydroxy-3-indoleacetic acid | ||||
16 | 1-甲基海因 1-methylhydantoin | 3.7246 | 4.2807 | 47 | 6-脱氧葡萄糖 6-Deoxyglucose | 1.0534 | 5.8862 |
17 | 赖氨酸 Lysine | 1.1595 | 3.5415 | 48 | 肌醇 Myo-inositol | 1.1812 | 6.4424 |
18 | 2-酮葡萄糖二甲基缩醛 | 1.7079 | 49 | 泛酸 Pantothenic acid | 1.5303 | 5.4521 | |
2-ketoglucose dimethylacetal | 50 | N-乙酰半乳糖胺 N-acetylgalactosamine | 4.0490 | ||||
19 | 己内酰胺 Epsilon-caprolactam | 2.6232 | 2.3184 | 51 | 四氧酸 Alloxanoic acid | 1.2308 | 3.9297 |
20 | 阿拉伯糖醇 Arabitol | 1.1096 | 52 | 绿原酸 Chlorogenic acid | 1.0311 | 3.9339 | |
21 | 胞嘧啶 Cytosin | 2.1537 | 53 | 瓜氨酸 Citrulline | 1.2555 | ||
22 | 亚油酸 Linoleic acid | 1.1261 | 54 | 鸟氨酸 Ornithine | 1.4025 | ||
23 | 琥珀酸 Succinic acid | 1.0970 | 55 | 胍丁胺 Agmatine | 2.2319 | ||
24 | 木糖 Xylulose | 1.0928 | 56 | α-生育酚 Alpha tocopherol | 2.5750 | ||
25 | 二十二碳烯酸 Docosenoic acid | 1.2319 | 57 | β-丙氨酸 Beta-alanine | 1.2123 | 2.3480 | |
26 | 1, 2, 3, 4, 5, 6, 6氘代-葡萄糖 | 1.1868 | 58 | 纤维二糖 Cellobiose | 1.4162 | 2.9658 | |
Glucose-1, 2, 3, 4, 5, 6, 6 deuterated | 59 | 核糖醇 Ribitol | 2.7145 | ||||
27 | 二十八酸 Montanic acid | 1.5310 | 60 | 2-(哌啶基)苯甲腈 | 2.3812 | ||
28 | 半乳酸 Galactonic acid | 1.1788 | 2.3643 | 2-piperidinobenzonitrile | |||
29 | 乳酸 Lactic acid | 1.1132 | 61 | 5-单磷酸胞苷 Cytidine-5-monophosphate | 3.6268 | ||
30 | 1, 2, 4-苯三酚 1, 2, 4-benzenetriol | 1.7583 | 62 | β-甘露醇甘油酯 Beta-mannosylglycerate | 3.1918 | ||
31 | 三糖 Trisaccharide | 2.3646 | 63 | 5-甲氧色胺 5-methoxytryptamine | 2.1717 | ||
32 | 肌苷 Inosine | 1.2861 | 64 | 腐胺 Putrescine | 2.1706 |
图2 辽粳433和越光糙米的差异代谢产物聚类分析的热图1~64同表1中序号,红色矩形表示代谢产物相对含量显著上调,蓝色矩形代表显著下调。Z1~Z6为越光的6次重复,Y1~Y6为辽粳433。
Fig. 2. Heat map of cluster analysis on differential metabolites of brown rice between Liaojing 433 and Koshihikari. The numbers 1-64 correspond to those in Table 1. The red rectangles indicate that the metabolite content is significantly up-regulated and the blue rectangles indicate the significant down-regulation of the metabolite content. Z1-Z6, Six repeats of Koshihikari; Y1-Y6, Six repeats of Liaojing 433.
差异代谢途径 Pathway name | 检测到的代谢物种类/参与此途径的代谢物总数 Detected/Total | 检测到的代谢产物种类Types of metabolites detected | ||
---|---|---|---|---|
辽粳433中相对含量高的代谢产物 Metabolites with relatively high contents in Liaojing 433 | 越光中相对含量高的代谢产物 Metabolites with relatively high contents in Koshihikari | |||
肌醇磷酸代谢Inositol phosphate metabolism | 1/17 | 肌醇 Myo-inositol | ||
抗坏血酸和醛酸代谢 Ascorbate and aldarate metabolism | 3/14 | 肌醇、抗坏血酸、尿苷二磷酸葡萄糖醛酸 Myo-inositol, ascorbic acid, uridine diphosphate glucuronic acid | ||
半胱氨酸和蛋氨酸代谢 Cysteine and methionine metabolism | 7/35 | 胱硫醚、丝氨酸、半胱氨酸、高丝氨酸、天冬氨酸、丙酮酸 Cystathionine, serine, cysteine, homoserine, aspartic acid, pyruvic acid | 甲硫氨酸 Methionine | |
赖氨酸生物合成 Lysine biosynthesis | 4/9 | 二氨基丙酸、天冬氨酸、高丝氨酸 Diaminopimelic acid, aspartic acid, homoserine | 赖氨酸 Lysine | |
泛酸盐和辅酶A生物合成 Pantothenate and CoA biosynthesis | 5/16 | β-丙氨酸、丙酮酸、泛酸 Beta-alanine, pyruvic acid, pantothenic acid | 缬氨酸、尿嘧啶Valine, uracil | |
β-丙氨酸代谢 beta-alanine metabolism | 5/12 | β-丙氨酸、天冬氨酸泛酸、亚精胺 Beta-alanine, aspartic acid, pantothenic acid, spermidine | 尿嘧啶 Uracil | |
嘌呤代谢 Purine metabolism | 10/55 | 谷氨酰胺、次黄嘌呤、硫酸盐、腺嘌呤、肌苷、鸟苷、尿囊酸、脱氧鸟苷 Glutamine, hypoxanthine sulfate, adenine, inosine, guanosine, allantoic acid, deoxyguanosine | 核酮糖-5-磷酸、尿酸Ribulose-5-phosphate, uric acid | |
苯丙烷生物合成 Phenylpropanoid biosynthesis | 5/31 | 酪氨酸、苯丙氨酸、4-羟基肉桂酸、阿魏酸、芥酸钠Tyrosine, phenylalanine, 4-hydroxycinnamic acid, ferulate, sinapate | ||
酪氨酸代谢 Tyrosine metabolism | 4/18 | 多巴、酪氨酸、琥珀酸、延胡索酸 Dopa, tyrosine, fumaric acid, succinic acid | ||
色氨酸代谢 Tryptophan metabolism | 4/25 | 羟色胺、吲哚乙酸、5-羟基吲哚乙酸 Serotonin, indoleacetic acid, 5-hydroxyindoleacetic acid | 色氨酸 Tryptophan | |
亚油酸代谢Linoleic acid metabolism | 1/5 | 亚油酸 Linoleic acid |
表2 辽粳433与越光糙米之间差异代谢途径和参与的代谢物
Table 2 Different metabolic pathways and involved metabolites between brown rice of Liaojing 433 and Koshihikari.
差异代谢途径 Pathway name | 检测到的代谢物种类/参与此途径的代谢物总数 Detected/Total | 检测到的代谢产物种类Types of metabolites detected | ||
---|---|---|---|---|
辽粳433中相对含量高的代谢产物 Metabolites with relatively high contents in Liaojing 433 | 越光中相对含量高的代谢产物 Metabolites with relatively high contents in Koshihikari | |||
肌醇磷酸代谢Inositol phosphate metabolism | 1/17 | 肌醇 Myo-inositol | ||
抗坏血酸和醛酸代谢 Ascorbate and aldarate metabolism | 3/14 | 肌醇、抗坏血酸、尿苷二磷酸葡萄糖醛酸 Myo-inositol, ascorbic acid, uridine diphosphate glucuronic acid | ||
半胱氨酸和蛋氨酸代谢 Cysteine and methionine metabolism | 7/35 | 胱硫醚、丝氨酸、半胱氨酸、高丝氨酸、天冬氨酸、丙酮酸 Cystathionine, serine, cysteine, homoserine, aspartic acid, pyruvic acid | 甲硫氨酸 Methionine | |
赖氨酸生物合成 Lysine biosynthesis | 4/9 | 二氨基丙酸、天冬氨酸、高丝氨酸 Diaminopimelic acid, aspartic acid, homoserine | 赖氨酸 Lysine | |
泛酸盐和辅酶A生物合成 Pantothenate and CoA biosynthesis | 5/16 | β-丙氨酸、丙酮酸、泛酸 Beta-alanine, pyruvic acid, pantothenic acid | 缬氨酸、尿嘧啶Valine, uracil | |
β-丙氨酸代谢 beta-alanine metabolism | 5/12 | β-丙氨酸、天冬氨酸泛酸、亚精胺 Beta-alanine, aspartic acid, pantothenic acid, spermidine | 尿嘧啶 Uracil | |
嘌呤代谢 Purine metabolism | 10/55 | 谷氨酰胺、次黄嘌呤、硫酸盐、腺嘌呤、肌苷、鸟苷、尿囊酸、脱氧鸟苷 Glutamine, hypoxanthine sulfate, adenine, inosine, guanosine, allantoic acid, deoxyguanosine | 核酮糖-5-磷酸、尿酸Ribulose-5-phosphate, uric acid | |
苯丙烷生物合成 Phenylpropanoid biosynthesis | 5/31 | 酪氨酸、苯丙氨酸、4-羟基肉桂酸、阿魏酸、芥酸钠Tyrosine, phenylalanine, 4-hydroxycinnamic acid, ferulate, sinapate | ||
酪氨酸代谢 Tyrosine metabolism | 4/18 | 多巴、酪氨酸、琥珀酸、延胡索酸 Dopa, tyrosine, fumaric acid, succinic acid | ||
色氨酸代谢 Tryptophan metabolism | 4/25 | 羟色胺、吲哚乙酸、5-羟基吲哚乙酸 Serotonin, indoleacetic acid, 5-hydroxyindoleacetic acid | 色氨酸 Tryptophan | |
亚油酸代谢Linoleic acid metabolism | 1/5 | 亚油酸 Linoleic acid |
[1] | Rehman H, Aziz T, Farooq M, Wakeel A, Rengel Z.Zinc nutrition in rice production systems[J]. Plant Soil, 2012, 361: 203-226. |
[2] | Mostafa K M, Quazi K H, Ehsan H C.Application of remote sensors in mapping rice area and forecasting its production: A Review[J]. Sensors, 2015, 15: 769-791. |
[3] | 景立权, 户少武, 穆海蓉, 王云霞, 杨连新. 大气环境变化导致水稻品质总体变劣[J]. 中国农业科学, 2018, 51(13): 2462-2475. |
Jing L Q, Hu S W, Mu H R, Wang Y X, Yang L X.Change of atmospheric environment leads to deterioration of rice quality[J]. Scientia Agricultura Sinica, 2018, 51(13): 2462-2475. (in Chinese with English abstract) | |
[4] | Ross M W, Robin D G.Breeding for micronutrients in staple food crops from a human nutrition perspective[J]. Journal of Experimental Botany, 2004, 55(396): 353-364. |
[5] | Uauy C, Distelfeld A, Fahima T, Blech A, Dubcovsky J.A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat[J]. Science, 2006, 314: 1298-1301. |
[6] | 成臣, 曾勇军, 程慧煌, 谭雪明, 商庆银, 曾研华, 石庆华. 齐穗至乳熟期不同温度对水稻南粳9108籽粒激素籽粒激素含量,淀粉积累及其合成关键酶活性的影响[J]. 中国水稻科学, 2019, 33(1): 57-67. |
Chen C, Zeng Y J, Cheng H H, Tab X M, Shang Q Y, Zeng Y H, Shi Q H.Effects of different temperature from full heading to milking on grain filling stage on grain hormones concentrations, activities of enzymes involved in starch synthesis and accumulation in rice Nanjing 9108[J]. Chinese Journal of Rice Science, 2019, 33(1): 57-67. (in Chinese with English abstract) | |
[7] | 周婵婵, 黄元财, 贾宝艳, 贾宝艳, 王岩, 李瑞峰, 王术, 冯跃, Dou F G.施氮量和灌溉方式的交互作用对东北粳稻米品质影响[J]. 中国水稻科学, 2019, 33(4): 357-367. |
Zhou C C, HuanY C, Jia B Y, Wang Y, Li R F, Wang S, Feng Y, Dou F G. Effect of interaction between nitrogen rate and irrigation regime on grain quality of japonica rice in northeast China[J]. Chinese Journal of Rice Science, 2019, 33(4): 357-367. (in Chinese with English abstract) | |
[8] | Cornejo F, Caceres P J, Martinez V C, Rosell C M, Frias J.Effects of germination on the nutritive value and bioactive compounds of brown rice breads[J]. Food Chemistry, 2015, 173: 298-304. |
[9] | Swati B P, Khalid K.Germinated brown rice as a value added rice product: A review[J]. Journal of Food Science and Technology, 2011, 48(6): 661-667. |
[10] | Nakabayashi R, Saito K.Metabolomics for unknown plant metabolites[J]. Analytical and Bioanalytical Chemistry, 2013, 405(15): 5005-5011. |
[11] | Feng Y C, Fu T X, Zhang L Y, Wang C Y, Zhang D J.Research on differential metabolites in distinction of rice origin based on GC-MS[J/OL].Journal of Chemistry, 2019: 1614504, doi: 10.1155/2019/1614504. |
[12] | Song E H, Jeong J, Park C Y, Kim H Y, Kin E H, Bang E J, Hong Y S.Metabotyping of rice for understanding its intrinsic physiology and potential eating quality[J]. Food Research International, 2018, 111: 20-30. |
[13] | Heuberger A L, Lewis M R, Chen M H, Brick M A, Leach J E, Ryan E P.Metabolomic and functional genomic analyses reveal varietal differences in bioactive compounds of cooked rice[J/OL].PLoS ONE, 2010, 5(9): e12915. |
[14] | 亓娜, 张欣, 施利利, 丁得亮, 崔晶, 王松文. 不同稻米食味及食味特性的比较[J]. 中国农学通报, 2013, 29(15): 204-208. |
Qi N, Zhang X, Shi L L, Di D L, Cui J, Wang S W.Study on different rice palatability characteristics[J]. Chinese Agricultural Science Bulletin, 2013, 29(15): 204-208. (in Chinese with English abstract) | |
[15] | 解文孝, 韩勇, 李建国, 沈峰, 刘博, 姜秀英, 刘军, 吕军, 蒋洪波, 唐志强, 张秀茹. 辽粳433跨区域栽培的适宜肥密运筹方式探讨[J]. 中国稻米, 2018, 24(3): 83-86. |
Xie W X, Han Y, Li J G, Shen F, Liu B, Jiang X Y, Liu J, Lü J, Jiang H B, Tang Z Q, Zhang X R.Discussion on appropriate fertilizer management methods for trans- regional cultivation of Liaojing 433[J]. China Rice, 2018, 24(3): 83-86. (in Chinese with English abstract) | |
[16] | Xia J G, Sinelnikov I V, Han B, Wishart D S.Metabo- Analyst 3.0: Making metabolomics more meaningful[J]. Nucleic Acids Research, 2015, 43: W251-W257. |
[17] | Zhao L J, Huang Y X, Hannah-Bick C, Fulton A N, Keller A A.Application of metabolomics to assess the impact of Cu(OH)2 nanopesticide on the nutritional value of lettuce(Lactuca sativa): Enhanced Cu intake and reduced antioxidants[J]. Nanolmpact, 2016(3-4): 58-66. |
[18] | 邹路易, 肖静静, 顾文秀, 李炜, 夏文水. 催化反应液中氨基葡萄糖酸的光度法测定[J]. 现代化工, 2011, 31(11): 93-95. |
Zou L Y, Xiao J J, Gu W X, Li W, Xia W S.New method for photometric determination of D-glucosaminic acid in catalytic reaction solution[J]. Modern Chemical Industry, 2011, 31(11): 93-95. (in Chinese with English abstract) | |
[19] | Ou S J, Chen G, Lin Z H, Bai Z P, Duan C Y, Mao C P.Chromium (Ⅲ) Complexes of D-glucosaminic acid and their effect on decreasing blood sugar in vivo[J]. Archiv der Pharmazie, 2006, 339(9): 527-530. |
[20] | 杨永超, 张海斐, 杨小振, 王中元, 魏春华, 张显. 甜瓜海藻糖-6-磷酸合成酶基因鉴定及表达[J]. 西北植物学报, 2017, 37(6): 1066-1072. |
Yang Y C, Zhang H F, Yang X Z, Wang Z Y, Wei C H, Zhang X.Expression analysis and genome-wide identification of trehalose-6-phosphate synthase gene family in melon (Cucumis melo L.)[J]. Acta Botanica Boreali-Occidentalia Sinica, 2017, 37(6): 1066-1072. (in Chinese with English abstract) | |
[21] | Kuivanen J, Dantas H, Mojzita D, Mallmann E, Biz A, Krieger N, Mitchell D A, Richard P.Conversion of orange peel to L-galactonic acid in a consolidated process using engineered strains of Aspergillus niger[J]. AMB Express, 2014, 4: 33. |
[22] | Semih O, Emine N T.Total dietary fiber intake, whole grain consumption, and their biological effects[J]. Bioactive Molecules in Food, 2019, 25: 701-722. |
[23] | Trompette A, Gollwitzer E S, Yadava k, Sichelstiel A K, Sprenger N, Ngom-Bru C, Blanchard C, Junt T, Ricod P, Harris N L, Marsland B J. Gut microbiota metabolism of dietary fiber influences allergic airway disease and hematopoiesis[J]. Nature Medicine, 2014, 20: 159-166. |
[24] | 王荣芳, 杨晓博, 安小楠, 崔同. 红枣低聚糖的HPLC分析[J]. 食品研究与开发, 2017, 38(15): 148-151. |
Wang R F, Yang X B, An X N, Cui T.HPLC analysis of oligosaccharides in jujube[J]. Food Research and Development, 2017, 38(15): 148-151. (in Chinese with English abstract) | |
[25] | 师文文, 吕攀, 徐庆强, 赵杰, 肖凯. 油酸在黄曲霉毒素诱导肝细胞损伤中的保护作用[J]. 中国油料作物学报, 2019, 41(2): 267-274. |
Shi W W, Lü P, Xu Q Q, Zhao J, Xiao K.Protective effect of oleic acid on aflatoxin-induced hepatocyte injury[J]. Chinese Journal of Oil Crop Sciences, 2019, 41(2): 267-274. (in Chinese with English abstract) | |
[26] | Khan A A, Alanazi A M, Jabeen M, Pervez K, Wahab R, Abdelhameed A S, Chauhan A.Biophysical interactions of novel oleic acid conjugate and its anticancer potential in heLa cells[J]. Journal of Fluorescence, 2015, 25(3): 519-525. |
[27] | Liu X H, Zeng X, Chen X M, Luo R X, Li L Z, Wang C S, Liu J P, Cheng J Q, Lu Y R, Chen Y N.Oleic acid protects insulin-secreting INS-1E cells against palmitic acid-induced lipotoxicity along with an amelioration of ER stress[J]. Endocrine, 2019, 64(3): 512-524. |
[28] | 张婧菲, 胡志萍, 王恬. 天然维生素E及其衍生物的研究进展[J]. 饲料工业, 2015, 8: 31-35. |
Zhang J F, Hu Z P, Wang T.Research progress of natural vitamin E and its analogues[J]. Feed Industry, 2015(8): 31-35. (in Chinese with English abstract) | |
[29] | Tigu F, Zhang J L, Liu G X, Cai Z, Li Y.A highly active pantothenate synthetase from Corynebacterium glutamicum enables the production of D-pantothenic acid with high productivity[J]. Applied Microbiology and Biotechnology, 2018, 102: 6039-6046. |
[30] | 李兴霖, 杨光, 赵平, 丁松乔. 微生物法测定婴幼儿配方乳粉中的泛酸含量[J]. 食品安全质量检测学报, 2017, 8(6): 2257-2262. |
Li X L, Yang G, Zhao P, Ding S Q.Detection of pantothenic acid in infant formula powders by microbiological method[J]. Journal of Food Safety and Quality, 2017, 8(6): 2257-2262. (in Chinese with English abstract) | |
[31] | Karalee D, Suriyong S.γ-Aminobutyric acid (GABA) content in different varieties of brown rice during germination[J]. Science Asia, 2012, 38: 13-17. |
[32] | 丁俊胄, 杨特武, 周强, 董梦钇, 熊善柏, 赵思明. 厌氧胁迫对发芽糙米中γ-氨基丁酸含量变化的影响[J].中国粮油学报, 2015(2): 6-10. |
Ding J Z, Yang T W, Zhou Q, Dong M Y, Xiong S B, Zhao S M.γ-aminobutyric acid content of brown rice induced by hypoxia stress during germination[J]. Journal of the Chinese Cereals and Oils Association, 2015(2): 6-10. (in Chinese with English abstract) | |
[33] | Smith A E, Walter A A, Graef J L, Kendall K L, Moon J R, Lockwood C M, Fukuda D H, Beck T W, Cramer J T, Stout J R.Effects of β-alanine supplementation and high-intensity interval training on endurance performance and body composition in men; a double-blind trial[J]. Journal of the International Society of Sports Nutrition, 2009, 6(1): 5. |
[34] | Saunders B, Sale C, Harris R C, Sunderland C.Effect of beta-alanine supplementation on repeated sprint performance during the loughborough intermittent shuttle test[J]. Amino Acids, 2012, 43: 39-47. |
[35] | Akeda K, Machida M, Kohara A, Oni N, Takemasa T.Effects of citrulline supplementation on fatigue and exercise performance in mice[J]. Journal of Nutritional Science and Vitaminology, 2011, 57(7): 246-250. |
[36] | Liu X, Zhang C C, Wang X R, Liu Q Q, Yuan D Y, Pan G, Sun S S M, Tu J M. Development of high-lysine rice via endosperm-specific expression of a foreign LYSINE RICH PROTEIN gene[J]. BMC Plant Biology, 2016, 16: 147. |
[37] | 马兆惠, 李坤, 程海涛, 陈云, 陈恒雪, 吕文彦. 表观直链淀粉和蛋白质双低型粳稻食味的关联性状分析[J]. 沈阳农业大学学报, 2019, 50(1): 10-18. |
Ma Z H, Li K, Cheng H T, Chen Y, Chen H X, Lü W Y.Correlation of low contents of apparent amylose and proteins with taste of japonica rice[J]. Journal of Shenyang Agricultural University, 2019, 50(1): 10-18. (in Chinese with English abstract) | |
[38] | 金正勋, 杨静, 钱春荣, 刘海英, 金学泳, 秋太权.灌浆成熟期温度对水稻籽粒淀粉合成关键酶活性及品质的影响. 中国水稻科学, 2005, 19(4): 377-380. |
Jin Z X, Yang J, Qian C R, Liu H Y, Jin X Y, Qiu T Q.Effects of temperature during grain filling period on activities of key enzymes for starch synthesis and rice grain quality[J]. Chinese Journal of Rice Science, 2005, 19(4): 377-380. (in Chinese with English abstract) | |
[39] | Shen Y, Jin L, Xiao P, Lu Y, Bao J S.Total phenolics, flavonoids, antioxidant capacity in rice grain and their relations to grain color, size and weight[J]. Journal of Cereal Science, 2009, 49(1): 106-111. |
[40] | Yu C H, Li Y, Zhao X, Yang S Q, Li L, Cui N X, Rong L, Yi C Z.Benzene metabolite 1, 2, 4-benzenetriol changes DNA methylation and histone acetylation of erythroid-specific genes in K562 cells[J]. Archives of Toxicology, 2019, 93(1): 137-147 |
[41] | 何华庆, 李思光, 琪寿. 奎尼酸生物合成的代谢工程[J]. 中国生物工程杂志, 2005, 25(11): 57-61. |
He H Q, Li S G, Xu Q S.Advances of metabolic engineering in biosynthesis of quinic acid[J]. China Biotechnology, 2005, 25(11): 57-61. (in Chinese with English abstract) | |
[42] | Hanson K R, Havir E A.Phenylalanine ammonia lyase//Conn E E. Secondary Plant Products: The Biochemistry of Plants [M]. New York: Academic Press, 1981, 7: 577-626. |
[43] | Maroli A S, Nandula V K, Dayan F E, Duke S O, Gerard P, Tharayil N.Metabolic profiling and enzyme analyses indicate a potential role of antioxidant systems in complementing glyphosate resistance in an Amaranthus palmeri biotype[J]. Journal of Agricultural and Food Chemistry, 2015, 63(1): 9199-9209. |
[44] | Xu J J, Fang X, Li C Y, Yang L, Chen X Y. General and specialized tyrosine metabolism pathways in plants[J/OL]. aBIOTECH, 2019, . |
[45] | 郑清岭, 杨忠仁, 张晓艳, 张凤兰, 郝丽珍. 干旱胁迫对沙芥和斧形沙芥抗坏血酸含量及其代谢相关酶的影响[J]. 植物生理学报, 2018, 54(12): 1865-1874. |
Zheng Q L, Yang Z R, Zhang X Y, Zhang F L, Hao L Z.Effects of drought stress on ascorbic acid contents and metabolism related enzymes of Pugionium cornutum and P. dolabratum[J]. Plant Physiology Journal, 2018, 54(12): 1865-1874. (in Chinese with English abstract) | |
[46] | 李京霞, 夏惠, 吕秀兰, 王进, 梁东. 抗坏血酸的代谢和调控: 以模式植物和园艺植物为例[J]. 中国生物工程杂志, 2018, 38(3): 105-114. |
Li J X, Xia H, Lü X L, Wang J, Liang D.The metabolism and regulation of ascorbic acid: A case study via model and horticultural plant[J]. China Biotechnology, 2018, 38(3): 105-114. (in Chinese with English abstract) | |
[47] | Raboy V.Approaches and challenges to engineering seed phytate and total phosphorus[J]. Plant Science, 2009, 177: 281-296. |
[48] | Kumar V, Sinha A K, Makkar H S, Becker K.Dietary roles of phytate and phytase in human nutrition[J]. Food Chemistry, 2010, 120: 945-959. |
[1] | 姚姝, 赵春芳, 陈涛, 路凯, 周丽慧, 赵凌, 朱镇, 赵庆勇, 梁文化, 赫磊, 王才林, 张亚东. 低谷蛋白半糯型粳稻营养品质与蒸煮食味品质特征分析[J]. 中国水稻科学, 2023, 37(2): 178-188. |
[2] | 潘阳阳, 陈宜波, 王重荣, 李宏, 黄道强, 周德贵, 王志东, 赵雷, 龚蓉, 周少川. γ-氨基丁酸和2-乙酰-1-吡咯啉代谢通路在水稻籽粒发育过程中的变化分析[J]. 中国水稻科学, 2021, 35(2): 121-129. |
[3] | 陈云, 张亚军, 张宏路, 朱安, 黄健, 张耗, 顾骏飞, 刘立军, 杨建昌. 机插株距对优质食味水稻品种产量和群体质量的影响[J]. 中国水稻科学, 2020, 34(6): 550-560. |
[4] | 胡标林, 黄得润, 肖叶青, 何强生, 万勇, 樊叶杨. 应用东乡野生稻回交重组自交系群体分析糙米矿质含量QTL[J]. 中国水稻科学, 2018, 32(1): 43-50. |
[5] | 刘奇华,蔡建,刘敏,柴廷友,李天. 两个籼稻品种垩白对稻米蒸煮食味与营养品质的影响[J]. 中国水稻科学, 2007, 21(3): 327-330 . |
[6] | 吴伟,程方民,刘正辉. 我国江浙地区粳稻品种间的植酸与蛋白质组分差异及其相关性[J]. 中国水稻科学, 2007, 21(3): 331-334 . |
[7] | 葛国科,张志,石春海,吴建国,叶子弘. 稻米直链淀粉和蛋白质含量对糙米重与外观品质性状间遗传相关性的影响[J]. 中国水稻科学, 2007, 21(1): 44-50 . |
[8] | 董明辉,桑大志,王朋,王学明,杨建昌. 不同施氮水平下水稻穗上不同部位籽粒的蒸煮与营养品质变化[J]. 中国水稻科学, 2006, 20(4): 389-395 . |
[9] | 孙成效, 段彬伍 ,谢黎虹,陈能. 利用近红外透射光谱技术同步测定糙米的多项品质指标初报[J]. 中国水稻科学, 2006, 20(4): 451-454 . |
[10] | 李钱峰, 刘巧泉,张达江,王红梅,于恒秀,顾铭洪,姚泉洪,. 转基因水稻中重组植酸酶的表达[J]. 中国水稻科学, 2006, 20(3): 243-247 . |
[11] | 李卫芬, 俞颂东, 孙建义. 添加NSP酶对早籼稻谷及其糙米体外消化的影响[J]. 中国水稻科学, 2004, 18(1): 86-88 . |
[12] | 贺建华,徐庆国,黄美华,金 宏,曾淑元. 饲料用稻谷和糙米的营养特性[J]. 中国水稻科学, 2000, 14(4): 229-232 . |
[13] | 石春海,余永贵,薛建明,杨肖娥,朱军. 籼稻稻米营养品质性状的种子、细胞质和母体遗传相关分析(英文)[J]. 中国水稻科学, 1996, 10(3): 143-146 . |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||