非洲栽培稻垩白粒率耐热性QTL的定位
#共同第一作者
收稿日期: 2019-07-26
修回日期: 2019-10-17
网络出版日期: 2020-03-10
基金资助
国家自然科学基金资助项目(31560382);江西省杰出青年科学基金资助项目(2018ACB21026);江西省自然科学基金资助项目(20151BAB214013;2017BAB204020);江西省现代农业科研协同创新专项(JXXTCXFY201902);国家重点研发计划资助项目(2017YFD0301601;2018YFD0301103);江西省重点研发计划资助项目(20171ACF60019)
QTL Mapping for Heat Tolerance of Chalky Grain Rate of Oryza glaberrima Steud.
#These authors contributed equally to this work
Received date: 2019-07-26
Revised date: 2019-10-17
Online published: 2020-03-10
【目的】本研究旨在定位一个稻米垩白粒率高温耐性QTL,为外观品质育种及解析垩白粒率高温耐性的遗传机制提供依据。【方法】以非洲栽培稻耐热品种IRGC102309(Oryza glaberrima Steud.)和籼稻品种R9311(O. sativa L. subsp. indica Kato.)为亲本构建的栽培稻种间染色体片段导入系CSIL05-23为材料构建次级分离群体,结合人工气候室模拟灌浆期高温胁迫处理,采用垩白粒率高温钝感值为评价指标,对非洲栽培稻垩白粒率高温耐性 QTL 进行检测。【结果】 在BC6F2分离群体,利用单标记分析,发现第5染色体上的SSR标记RM1200与垩白粒率耐热性状极显著正相关（P=0.0005）。进一步利用BC6F3和BC6F4分离群体,采用QTL Cartographer 2.5软件和复合区间作图法在水稻第5染色体上的SSR标记RM1200-RM5796区间重复检测到一个灌浆期垩白粒率耐热性QTL, 命名为qHTCGR5,分别解释11.4%和17.5%表型变异。根据BC6F4分离群体的纯合重组体表型分组,利用置换作图方法将目标QTL同样定位在SSR标记RM1200-RM5796之间,遗传图距为1.3 cM,物理图距约为333.4 kb。【结论】 控制垩白粒率耐热性的qHTCGR5是一个能够用于稻米外观品质育种的新QTL。
曹志斌, 李瑶, 曾博虹, 毛凌华, 蔡耀辉, 吴晓峰, 袁林峰 . 非洲栽培稻垩白粒率耐热性QTL的定位[J]. 中国水稻科学, 2020 , 34(2) : 135 -142 . DOI: 10.16819/j.1001-7216.2020.9086
【Objective】The objective of the research is to identify a QTL for heat tolerance of chalky grain rate, so as to provide support for the appearance quality breeding and analysis of genetic mechanism of rice high temperature tolerance of chalky grain rate. 【Method】 We developed a chalky grain rate heat-tolerant CSIL(chromosomal segment introgression lines), CSIL05-2, by backcrossing and marker assisted selection with African cultivated rice acc. IRGC102309 (Oryza glaberrima Steud.) as donor parent and Asian cultivated rice R9311 (O. sativa L. subsp. indica Kato) as recipient parent. And a QTL for insensitity of heat tolerance of chalky grain rate on chromosome 5 was analyzed using secondary populations from CSIL05-2.【Results】In a BC6F2 segregation populations, the SSR marker RM1200 on chromosome 5 showed significant correlation with heat tolerance of chalky grain rate by single marker analysis（P=0.0005）. Using Cartographer 2.5 and the composite interval mapping with BC6F3 and BC6F4 populations, we further anchored the quantitative trait loci (QTL) associated with heat tolerance of chalky grain rate at filling stage to the same position within a 1.3 cM interval, which was designed as qHTCGR and explained 11.4% and 17.5% of the phenotypic variances, respectively. Using homozygous recombinants screened from BC6F4 populations, qHTCGR5 was also detected in a ~333.4 kb region between RM11633 and RM11642.【Conclusion】 The QTL controlling the heat tolerance of chalky grain rate qHTCGR5 is a novel QTL.
Key words： rice; chalky grain rate; heat tolerance; QTL; gene mapping
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