硫化氢提高水稻磷吸收转运的生理和分子机制
收稿日期: 2019-05-13
修回日期: 2019-07-31
网络出版日期: 2019-11-10
基金资助
国家重点研发计划资助项目(2016YFD0101801);国家自然科学基金面上项目(31872857);浙江省自然科学基金资助项目(LY18C020005);国家公益研究所基础研究基金资助项目(2017RG004-2)
Physiological and Molecular Mechanisms of Hydrogen Sulfide Enhancing Phosphorus Absorption and Transportation in Rice
Received date: 2019-05-13
Revised date: 2019-07-31
Online published: 2019-11-10
目的 低磷胁迫是限制水稻产量的主要因素之一。水稻淹水条件下产生H2S,然而,H2S作为信号分子是否参与调节水稻响应缺磷胁迫还未可知。方法 在正常磷和低磷条件下测定水稻H2S含量,揭示H2S在水稻响应缺磷胁迫中的作用。用2 μmol/L H2S前体物质NaHS预处理水稻1 d,然后在加磷和低磷条件下培养6 d,测定水稻体内总磷含量、酸性磷酸酶活性、抗氧化酶活性、木质部汁液磷含量、磷转运子基因表达以及根系构型变化,从而探究H2S参与调节水稻响应缺磷胁迫的生理和分子机制。结论 低磷胁迫下,水稻根系和地上部H2S含量显著增加。NaHS预处理水稻显著增加低磷条件下水稻体内有效磷和总磷含量,提高根系酸性磷酸酶活性,提高抗氧化酶活性、木质部汁液磷含量和磷转运子基因表达水平,同时还改变水稻根系构型,增加总根长、总根表面积、总根体积和总根尖数,从而促进低磷条件下水稻对外界磷的吸收和转运,最终缓解缺磷胁迫。
朱春权, 曹小闯, 朱练峰, 白志刚, 黄洁, 梁清铎, 金千瑜, 张均华 . 硫化氢提高水稻磷吸收转运的生理和分子机制[J]. 中国水稻科学, 2019 , 33(6) : 532 -540 . DOI: 10.16819/j.1001-7216.2019.9055
【Objective】 Low phosphorus (P) stress is one of the main factors limiting rice yield. Hydrogen sulfide (H2S) is produced in rice under flooding conditions. However, whether H2S is involved in the regulation of rice response to phosphorus deficiency stress as a signal molecule is still unclear. 【Method】 The content of H2S in rice was determined under normal and low P conditions to illuminate the role that H2S plays in regulating rice response to P deficiency stress. Rice was pretreated with 2 μmol/L H2S precursor NaHS for 1 day, then cultured for another 6 days under P sufficient and deficient conditions. The total P contents, acid phosphatase activity, antioxidant enzyme activity, xylem P concentration, P transporter genes’ expression and rice root architecture were determined to explore the physiological and molecular mechanism of H2S in regulating rice response to phosphorus deficiency stress. 【Conclusion】 Low P stress significantly increased the content of H2S in rice roots and shoots. NaHS pretreatment significantly increased total P contents, root acid phosphatase activity, root antioxidant enzymes activity, xylem P concentration and stimulated the expression of P transporter genes. Meanwhile, NaHS pretreatment modified rice root architecture, including increased total root length, total root surface area, total root volume and total root tip numbers. Therefore, our present study demonstrated that H2S improved P absorption and translocation in rice under P deficiency conditions and finally alleviate P deficiency stress in rice.
Key words： rice; P deficiency stress; H2S; P transporter; root architecture
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