目的 磷对植物细胞内蔗糖和淀粉合成有重要影响,它们之间的关系还有许多方面有待阐明。方法 本研究利用转基因技术获得OsSUT1超表达材料,通过水培和盆栽实验研究了超表达该基因对蔗糖、磷含量以及植株形态和生理性状的影响。结果 转录水平上的表达分析显示,缺磷诱导OsSUT1在水稻根系中表达。水培实验显示,与野生型相比,正常供磷条件下OsSUT1超表达导致水稻植株地上部蔗糖含量下降,同时水稻植株内的磷含量升高;而在缺磷条件下植株体内蔗糖及磷含量均无显著变化。盆栽实验显示,超表达OsSUT1提高了水稻的分蘖数、有效分蘖数、磷含量、粒长和粒宽。结论 这些结果说明OsSUT1对水稻的蔗糖和磷含量以及种子的生长发育有重要作用。

【Objective】Phosphorus exerts an important influence on synthesis of sucrose and starch in plant cell. There are still many aspects need to be clarified about their relationship.【Method】In this study, OsSUT1-overexpressed material was obtained by using transgenic technology. Effects of overexpression of this gene on sucrose and phosphorus contents, as well as plant morphology and physiology were investigated under hydroponics and pot experiment conditions. 【Result】Expression analysis at the transcription level indicated that OsSUT1 was induced by phosphorus deficiency in rice roots. Hydroponics experiment showed that compared with the wild-type plant, over-expression of OsSUT1 in the transgenic plant under normal phosphorus supply resulted in a decrease in sucrose content in shoot and an increase in phosphorus content in both root and shoot. However, there was no significant difference in sucrose and phosphorus contents between the OsSUT1-overexpressed and control plants under phosphorus deficiency conditions. Pot experiment showed that overexpression of OsSUT1 increased the number of tillers, effective tillers, and phosphorus content in rice, and the seed size was also larger than that of the wild type. 【Conclusion】These results indicate that OsSUT1 influences sucrose and phosphorus contents and growth and development of the transgenic rice.