水稻耐金属离子胁迫的QTL分析
共同第一作者:林晗, 徐江民, 胡瑚倩；
收稿日期: 2017-06-20
修回日期: 2017-08-21
网络出版日期: 2018-01-10
基金资助
国家转基因生物新品种培育重大科技专项(2016ZX08009003-003-008);浙江省自然科学基金资助项目(LY16C130001);浙江省科协育才工 程资助项目(2017YCGC008);水稻生物学国家重点实验室开放资助项目
Identifying of QTLs for Resistance to Metal Irons Stress in Rice
These authors contributed equally to this work:LIN Han, XU Jiangmin, HU Huqian;
Received date: 2017-06-20
Revised date: 2017-08-21
Online published: 2018-01-10
【目的】 本研究旨在筛选与水稻苗期耐不同金属离子连锁的分子遗传标记,为探讨水稻耐不同金属离子胁迫的遗传研究提供参考。【方法】 以典型籼粳交(春江06/台中本地1号)双单倍体(DH)群体为材料,系统考查该群体及其双亲耐4种金属离子(Fe2+、Cd2+、Al3+、Na+)胁迫的情况,利用业已构建并完善的该群体加密的分子连锁图谱,对耐这4种金属离子胁迫的QTL进行检测分析。另外,利用实时定量PCR技术检测处理前后相关基因的表达变化情况。【结果】 发现耐各种金属离子胁迫的QTL共8个,分别位于水稻第1、2、4、6、9、10和11染色体上,其中Fe2+处理后检测到的QTL贡献率最大,达到24.47%(阈值为7.78),位于第1染色体上RM1297–RM1061,同时对该区间与耐胁迫相关基因的表达分析发现这些基因在处理前和处理后表达水平存在不同程度的差异;Cd2+处理后检测到1个QTL,位于第1染色体上;Al3+处理后检测到QTL共5个,分别位于第2、4、6、10、11染色体上;Na+处理后检测到QTL有1个,位于第9染色体上。【结论】 根据不同金属离子胁迫处理后DH群体的表型差异进行QTL分析,发现耐各种金属离子胁迫的QTL共8个,并初步定位于各染色体的遗传标记区间,这为精细定位并克隆相应QTL,进而探明水稻耐金属离子胁迫QTL的分子调控机制奠定了基础。
林晗, 徐江民, 胡瑚倩, 郑安, 徐婉璐, 漏平, 王跃星, 曾大力, 饶玉春 . 水稻耐金属离子胁迫的QTL分析[J]. 中国水稻科学, 2018 , 32(1) : 23 -34 . DOI: 10.16819/j.1001-7216.2018.7075
【Objective】 The molecular markers linked to the tolerance of various metal ions during rice seeding stage were screened for the purpose of discussing the genetic basis of the resistance to different metal ions.【Method】 A rice double haploid(DH) population, derived from a typical indica-japonica cross(Chunjiang 06/TN1) via the anther culture was used to analyze the resistance of four metal ions(Fe2+, Cd2+, Al3+, Na+) in DH population and its parents, then the quantitative trait loci (QTL) for the resistance of the four metal ions were identified using an available and complete molecular linkage map. In addition, real-time quantitative PCR was used to detect the expression of related genes before and after treatment.【Result】 A total of 8 QTLs for resistance to metal ions stress were detected, which were localized on chromosomes 1, 2, 4, 6, 9, 10 and 11. Among them, one QTL with the greatest contribution for resistance to Fe2+ treatment(24.47%, threshold is 7.78) located in the region RM1297–RM1061 on chromosome 1. Meanwhile, the expression analysis of the genes related to stress tolerance in this interval showed that these genes had different expression levels before and after treatment. One QTL was detected after Cd2+ treatment, which was located on chromosome 1. Five QTLs were detected after Al3+ treatment, which were located on chromosomes 2, 4, 6, 10 and 11 respectively. And one QTL was detected after Na+ treatment, which was located on chromosome 9. 【Conclusion】 According to the QTL analysis of phenotypic differences among DH groups which were treated with different metal ions, eight QTLs for resistance to various metal ions were detected. It laid the foundation for the fine mapping and cloning of the corresponding QTL and exploring the QTL molecular regulation mechanism of the resistance to different metal ions in rice.
Key words： rice; resistance; metal ion stress; QTL analysis
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