*通讯录作者：E-mail:wuxjsau@126.com,caolycgf@mail.hz.zj.cn
收稿日期: 2015-02-09
修回日期: 2015-09-06
网络出版日期: 2015-11-10
基金资助
中央级公益性科研院所基本科研业务费专项(2014RG002-7)中国农业科学院水稻杂种优势机理研究创新团队项目(CAAS-ASTIP-2013-CNRRI)
Gene Mapping of a Narrow and Rolled Leaf Mutant Nrl3(t) in Rice
*Corresponding author：E-mail:wuxjsau@126.com,caolycgf@mail.hz.zj.cn
Received date: 2015-02-09
Revised date: 2015-09-06
Online published: 2015-11-10
利用60Co-γ射线辐射诱变籼稻恢复系中恢8015,获得了一份窄卷叶突变体Nrl3(t)。与野生型中恢8015相比,突变体叶片显著变窄内卷、株高降低、穗长变短、结实率降低、千粒重降低、粒长粒宽减小。细胞学分析表明,突变体维管束减少和泡状细胞不够饱满是导致叶片变窄卷曲的原因。遗传分析表明该突变体受一对隐性核基因控制,以窄卷叶突变体Nrl3(t)与粳稻品种02428杂交获得的F2分离群体作为定位群体,利用SSR标记和新开发的InDel标记,将窄卷叶基因Nrl3(t)定位于第2染色体长臂标记C9和C10E之间约70.3 kb区间内。研究结果为该基因的克隆及进一步揭示水稻窄卷叶形成的分子机理奠定了基础。
张晓惠, 秦亚芝, 张迎信, 占小登, 张振华, 沈希宏, 程式华, 曹立勇, 吴先军 . 水稻窄卷叶突变体Nrl3(t)的基因定位[J]. 中国水稻科学, 2015 , 29(6) : 595 -600 . DOI: 10.3969/j.issn.1001G7216.2015.06.005
Nrl3(t) was a novel narrow and rolled leaf mutant from the indica restorer line Zhonghui 8015 after exposure to 60Co-γ ray. Nrl3(t) exhibited significant adaxially-rolled leaves, reduced plant height, shortened panicle length, reduced grain length, grain width and thousand-grain weight, as well as the seed-setting rate. Cytological analysis showed that the rolled leaf phenotype was due to the reduced number of vascular bundleand the changed size of bulliform cells. In an initial mapping with F2 individuals derived from a cross between Nrl3(t) and japonica 02428, the narrow and rolled leaf gene Nrl3(t) was eventually mapped to a 70.3 kb region on the long arm of chromosome 2 between two newly developed sequence tagged site markers C9 and C10E. These results would lay a good foundation for molecular cloning and functional analysis of Nrl3(t).
Key words： rice; narrow and rolled leaf; gene; fine mapping
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