利用选择导入系进行水稻耐低磷鉴定与QTL定位分析
收稿日期: 2014-04-28
修回日期: 2014-05-08
网络出版日期: 2015-01-10
基金资助
国家863计划资助项目(2014AA10A604);比尔和梅琳达盖茨基金资助项目(OPPGD1393)
Dissection and QTL Mapping of Low-phosphorus Tolerance Using Selected Introgression Lines in Rice
Received date: 2014-04-28
Revised date: 2014-05-08
Online published: 2015-01-10
选取我国当前生产上大面积推广应用的籼稻恢复系明恢86和蜀恢527作为轮回亲本,以籼稻品种爷驼崽为供体亲本构建了2个BC2F4群体,在河北廊坊田间正常土壤正常施肥(对照)、贫瘠土壤正常施肥和贫瘠土壤低磷胁迫这三种处理下进行产量及相关性状的表型评价。同时,在北京温室采用水培法进行苗期耐低磷鉴定。在廊坊检测到49个产量及相关性状主效QTL,这些QTL对表型变异的贡献率为6.7%~16.5%; 其中有25个(51.0%) QTL的有利等位基因来自供体亲本爷驼崽。在北京检测到影响耐低磷相关性状(根长、根系干质量、根冠比、地上部分干质量和总干质量)的主效QTL 48个,这些QTL对表型变异的贡献率为7.7%~16.6%; 其中有21个(43.8%) QTL的有利等位基因来自供体亲本爷驼崽。多达79.6%的QTL能在廊坊环境两个或两个以上处理下被检测到,特别是与每穗总粒数、结实率和千粒重有关的QTL,不仅能稳定的表达,并且它们在不同处理下具有比较一致的效应。在检测到的所有QTL中,有8个染色体区段在两种环境的低磷胁迫条件下被同时检测到,其中与第12染色体RM511(Bin12.4)紧密连锁的位点,同时控制每穗实粒数、每穗总粒数、根长、根系干质量、地上部分干质量和总干质量等6个性状。
任洁, 赵秀琴, 丁在松, 项超, 张晶, 王超, 张俊巍, 张强, 庞昀龙, 高用明, 石英尧 . 利用选择导入系进行水稻耐低磷鉴定与QTL定位分析[J]. 中国水稻科学, 2015 , 29(1) : 1 -13 . DOI: 10.3969/j.issn.1001-7216.2015.01.001
Two BC2F4 backcrossing populations were generated by using the two widely used elite indica restorers in China, Shuhui 527 and Minghui 86 as recurrent parents, the indica variety Yetuozai as donor. The grain yield and its related traits were evaluated under normal fertilizer application level in normal soil (as control) and in poor soil, low phosphorus stress conditions in poor soil in Langfang, Hebei Province. Meanwhile, the introgressed lines (ILs) of two populations were investigated at seedling stage using nutrient solution in the glasshouse in Beijing. A total of 49 main-effect QTLs underlying yield related traits were identified, the contribution of phenotypic variations ranged from 6.7% to 16.5%. Of which, 25 (51.0%) QTLs had favorable alleles from donor parent. A total of 48 main-effect QTLs were identified for yield related traits, the contribution of phenotypic variations ranged from 7.7% to 16.6%. Of which, 21(43.8%) QTLs had favorable alleles from donor parent. About 79.6% of the QTLs can be detected repeatedly under two or more treatments, especially those QTLs associated with grain number per panicle, spikelet fertility and thousand grain weight which displayed consistent phenotypic effects. Among all detected QTLs, eight could be simultaneously identified across two environments. RM511(Bin.12.4) on chromosome 12 showed the most strong pleiotropism, simultaneously controlling filled grain weight, total grain weight per panicle, root length, dry weight of root, dry weight of shoot and total dry weight.
| [1] | Vinod K K, Heuer Sigrid.Approaches towards nitrogen- and phosphorus-efficient rice.AoB Plants, 2012, doi:10.1093/aobpla/pls028. |
| [2] | Carriger S, Vallee D.More crop per drop.Rice Today, 2007, 6: 10-13. |
| [3] | 吴平. 植物磷高效吸收利用的分子机制研究及其应用. 中国科技成果, 2013 (9): 20-20. |
| [4] | 穆平, 黄超, 李君霞, 等.低磷胁迫下水稻产量性状变化及其定位. 作物学报, 2008, 34(7):1137-1142 |
| [5] | Loudet O, Chaillou S, Merigout P, et al.Quantitative trait loci analysis of nitrogen use efficiency in Arabidopsis.Plant Physiol, 2003, 131(1): 345-358. |
| [6] | 杜娟, 曾亚文, 杨树明, 等.水稻磷高效重组自交系群体的筛选鉴定. 生态环境, 2008, 17(3): 1151-1156. |
| [7] | Tanksley S D, Nelson J C.Advanced backcross QTL analysis: A method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines.Theor Appl Genet, 1996, 92(2): 191-203. |
| [8] | Li Z K, Fu B Y, Gao Y M, et al.Genome-wide introgression lines and their use in genetic and molecular dissection of complex phenotypes in rice (Oryza sativa L.).Plant Mol Biol, 2005, 59(1): 33-52. |
| [9] | 罗利军. 水稻等基因导入系构建与分子技术育种.分子植物育种, 2005, 3(5): 609-612. |
| [10] | Chen M Y, Ali J, Fu B Y, et al.Detection of drought-related loci in rice at reproductive stage using selected introgressed lines.Agric Sci China, 2011, 10(1): 1-8. |
| [11] | Moncada P, Martinez C P, Borrero J, et al.Quantitative trait loci for yield and yield components in an Oryza sativa-Oryza rufipogon BC2F2 population evaluated in an upland environment.Theorl Appl Genet, 2001, 102(1): 41-52. |
| [12] | GRAMENE. GRAMENE Database.[2006-4-5] |
| [13] | 郑天清, 徐建龙, 傅彬英, 等.遗传搭车与方差分析在水稻定向选择群体的抗旱性位点分析中的初步应用.作物学报, 2007, 33(5): 799-804. |
| [14] | SAS Institute Inc.ed., 2008, SAS/STAT V9.2 user’s guide, SAS Institute Inc, Cary, NC, USA: 2430-2611. |
| [15] | 高方远, 陆贤军, 康海岐, 等.水稻耐低磷种质的苗期筛选与鉴定.作物学报, 2006, 32(8): 1151-1155. |
| [16] | 张帆,郝宪彬,高用明, 等.利用籼稻资源中的“隐蔽有利基因”提高粳稻苗期耐冷性.作物学报, 2007, 33(10): 1618-1624. |
| [17] | He Y X, Zheng T Q, Hao X B, et al.Yield performances of japonica introgression lines selected for drought tolerance in a BC breeding programme.Plant Breeding, 2010, 129(2): 167-175. |
| [18] | Meng L J, Lin X Y, Wang J M, et al.Simultaneous improvement of cold tolerance and yield of temperate japonica rice (Oryza sativa L.) by introgression breeding.Plant Breeding, 2013, 132(6): 604-612. |
| [19] | 徐建龙,高用明,傅彬英,等.回交导入后代水稻种质有利基因的鉴定与筛选研究. 分子植物育种, 2005, 3(5): 619-628. |
| [20] | 黎志康. 我国水稻分子育种计划的策略. 分子植物育种, 2005, 3(5): 603-608. |
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