中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻纹枯病菌拮抗菌B34分离鉴定及杀菌蛋白的获得

郑爱萍; 李 平 *; 王世全; 王玲霞; 马炳田   

  1. 四川农业大学 水稻研究所， 四川农业生物技术工程研究中心， 四川 温江 611130； *通讯联系人，E-mail:liping@mail.sicau.edu.cn
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-10-10 发布日期:2002-10-10

Screening,Taxonomy of Antagonistic Strain B34 Against Rhizoctonia solani and Acquirement of the Antiseptic Protein

ZHENG Ai-ping; LI Ping; WANG Shi-quan; WANG Ling-xia; MA Bing-tian   

  1. (Rice Research Institute; Sichuan Agricultural University; Sichuan Agricultural Biotechnology Engineering Research Center; Wenjiang 611130; China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-10-10 Published:2002-10-10

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摘要： 从水稻植株上分离到1株对水稻纹枯病菌有较好拮抗作用编号为B34的菌株，室内对水稻纹枯病菌的抑菌带达到10.3 mm。经生理、生化检测及细胞壁化学成分分析，确定其为致黄假单胞菌（Pseudomonas aureofaciens）。其代谢粗提物经蛋白酶作用，拮抗作用消失。经70%饱和度的（NH4）2SO4沉淀，透析后得到粗杀菌蛋白，分别上样SephadexG-100和DEAE纤维素32LKB柱层析系统，分离纯化了杀菌蛋白，经SDS-PAGE检测其分子量约为33.0 kDa，经低温、真空干燥，得到了该杀菌蛋白的结晶体。

关键词: 水稻纹枯病, 拮抗作用, 生物防治, 杀菌蛋白, 致黄假单胞菌

Abstract: A strain B34 against Rhizoctonia solani was screened from rice plant,in vitro the inhibition zone width was 10 3 mm. Results of analysis on chemical components in cell wall, physiological and biochemical characters of B34, indicated it was Pseudomonas aureofaciens. When its crude extracts were treated by proteinase K and trypsin, the antagonism disappeared. The crude protein of B34 was obtained with 70% staturated (NH4)2SO4. The protein was purified by ion-exchange and gel filtration LKB chromatography. One antagonistic peak was attained and showed only single band with 33.0 kDa by SDS-PAGE. The purified protein crystal of antiseptic protein was obtained.

Key words: Rhizoctonia solani, antagonism, biological control, antiseptic protein, Pseudomonas aureofaciens