中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻抗白叶枯病微效QTL的定位分析

于彦春1,2,3; 滕胜1,2; 曾大力2; 董国军2; 钱前2,*; 黄大年2; 朱立煌4,*   

  1. 1浙江大学 生命科学学院,　浙江 杭州　310029； 2中国水稻研究所 农业部水稻生物学重点实验室， 浙江　杭州　310006; 3吉林农业大学 生物技术学院， 吉林 长春 130118； 4中国科学院 遗传与发育生物学研究所， 北京　100101；
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-10-10 发布日期:2003-10-10

Analysis of QTLs for Resistance to Rice Bacterial Blight

YU Yan-chun 1; 2; 3; TENG Sheng 1; ZENG Da-li 2; DONG Guo-jun 2; QIAN Qian 2; *; HUANG Da-nian 2; ZHU Li-huang 4; *   

  1. （ 1College of Life Science; Zhejiang University; Hangzhou 310029; China; 2Key Laboratory for Rice Biology; Ministry of Agriculture; China National Rice Research Institute; Hangzhou 310006; 3College of Biotechnology; Jilin Agricultural University; Changchun 130118; 4Institute of Genetics and Developmental Biology; Chinese Academy of Sciences; Beijing 100101, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-10-10 Published:2003-10-10

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摘要： 以不携有抗白叶枯病主效基因的中感籼粳双亲(窄叶青8号和京系17）及其花培DH群体为材料，接种白叶枯病菌浙9612后，考察了该DH群体的白叶枯病抗性，并进行了数量性状座位（QTL）分析。共检测到控制白叶枯病的4个QTL，分别位于第3、4、5和6染色体上，其中第3和第4染色体上的qBBR-3和qBBR-4，其加性效应为正值；而位于第5和第6染色体上的为负值，4个QTL的总效应是38.6%。方差分析和差异显著性比较表明，具有4个QTL抗性等位基因水稻株系的白叶枯病抗性与不含QTL抗性等位基因的株系相比，差异达极显著水平（P<0.01)。说明通过微效基因的聚合可以获得对白叶枯病的一定抗性。

关键词: 数量性状座位, 加倍单倍体群体, 白叶枯病, 抗病性

Abstract: A japonica/indica double haploid(DH) population, derived from Zhaiyeqing 8 and Jingxi 17, was used to analyze QTLs for resistance to rice bacterial blight (BBR). Both the parents and DH population did not carry any major gene and the parents were moderately susceptible to strain of Xanthomonas oryzae pv. oryzae(Xoo), Zhe 9612. Four QTLs (qBBR-3, qBBR-4, qBBR-5, and qBBR-6) were detected for BBR on chromosome 3, 4, 5 and 6, respectively. qBBR-3 and qBBR-4 showed additive effect, and qBBR-5 and qBBR-6 negative, the total effect of four QTLs was 38.6%. The variance analysis and significance test showed that the BBR effect of the rice lines with four QTLs was highly significant, and the resistance to Xoo strain could be attained by pyramiding minor genes.

Key words: quantitative trait loci, double haploid population, rice bacterial blight, resistance to disease, rice