中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    下一篇

几种野生稻抽提物对斜纹夜蛾幼虫的拒食作用

冯国忠1; 万树青1;*; 潘大建2; 罗 倩1; 邓建朝1   

  1. 1华南农业大学 昆虫毒理研究室, 广东 广州 510642; 2广东省农业科学院 水稻研究所, 广东 广州 510640
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-07-10 发布日期:2004-07-10

Antifeedant Activity of the Extracts of Six Species of Wild Rice Aganist Spodoptera litura

FENG Guo-zhong 1; WAN Shu-qing 1; *; PAN Da-jian 2; LUO Qian 1; DENG Jian-chao 1   

  1. ( 1 Laboratory of Insect Toxicology; South China Agricultural University; Guangzhou 510642; China; 2Rice Research Institute; Guangdong Academy of Agricultural Sciences; Guangzhou 510640; China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-07-10 Published:2004-07-10

PDF

摘要： 用甲醇对6种野生稻E6-1, E6-3/6-4(大颖野生稻的不同生态型),E7-4(颗粒野生稻), E9-10、E9-20(阔叶野生稻的不同生态型),E13-13（小粒野生稻）,E15-13（药用野生稻）和E16-3（斑点野生稻）进行了索氏提取，提取率分别为19.62%,15.60%,18.90%,8.50%,16.27%, 24.86%,10.04%和13.32%。通过测定这6种野生稻抽提物对3龄斜纹夜蛾幼虫的选择性拒食活性和非选择性拒食活性，筛选出大颖野生稻抽提物E6-1对斜纹夜蛾有较强的拒食作用。用E6-1对叶碟处理后，在1%的浓度下，24 h和48 h测得其对3龄斜纹夜蛾选择性拒食率均为100%，而非选择性拒食率分别为40.95%和47.27%；毒力测定表明2龄斜纹夜蛾幼虫24 h和48 h后的拒食中浓度([i]AFC[/i][sub]50[/sub]）分别为120 mg/mL和50 mg/mL。

关键词: 野生稻, 抽提物, 斜纹夜蛾, 选择性拒食活性, 非选择性拒食活性

Abstract: Six species of wild rice E6-1, E6-3/6-4 (different ecotypes of Oryza grandiglumis), E7-4(O. granulata), E9-10, E9-20(different ecotypes of O. latifolia),E13-13(O. minuta), E15-13 (O. officinalis) and E16-3(O. punctata) were extracted with methnol by Sohxlet method. The extract rates of the six species of wild rice were 19.62%,15.60%,18.90%,8.50%,16.27%, 24.86%,10.04% and 13.32%, respectively. Selective antifeedant activity and non-selective antifeedant activity of extract from the six species of wild rice against the 3rd instar larvae of Spodoptera litura showed that the extract of the wild rice E6-1 was better than the others.When the concentration was 1%, selective antifeedant activity of the E6-1 extracts was 100% in 24 h and 48 h and non selective antifeedant activity was 40 95% and 47 27% in 24 h and 48 h after treatment, respectively.The concentration of 50% antifeedant of the wild rice E6 1 extract against 2nd instar larvae of Spodoptera litura was 120 mg/mL in 24 h and 50 mg/mL in 48 h after treatment.

Key words: wild rice, extract, Spodoptera litura, selective antifeedant activity, non selective antifeedant activity