Abstract:
Secreted proteins during spore germination of Magnaporthe oryzaewere identified. Ungerminated spores, germinated spores and the mixture of germinated spores with germination liquid of M. oryzae (200550, 200581) were inoculated on rice seedlings, respectively. The disease indices were 1113, 3506, 4901 and 4901, 84, 627, indicating that germination liquid played a driving role in infection process. SDSPAGE amplification revealed that secreted proteins were contained. According to the results of mass spectrometer, and database query, eight genes were involved, including MGG_01305, MGG_13188, MGG_12454, etc. The results indicated that these proteins played key roles and important function during M. oryzae infection.
LIU Yong-Feng,CHEN Zhi-Yi,YU Wen-Yuan et al. Primary Study on Effect of Secreted Proteins of Magnaporthe oryzae During Infection[J]. , 2011, 25(4): 452-454.
Liu Y F, Chen Z Y, Hu M, et al. Distribution of Magnaporthe oryzae populations and virulence of predominant races in Jiangsu Province. Rice Sci,2004, 11(5/6): 324-330.
[6]
Zhou E X, Jia Y L, Singh P, et al. Instability of the Magnaporthe oryzae avirulence gene AVR-Pita alters virulence. Fungal Genet Biol, 2007, 44: 1024-1034.
[7]
Chen X W, Shang J J, Chen D X, et al. A B-lectin receptor kinase gene conferring rice blast resistance. Plant J,2006, 46: 794-804.
Rubio M B, Cardoza R E, Hermosa R, et al. Cloning and characterization of the Thcut1 gene encoding a cutinase of Trichoderma harzianumT34. Curr Genet,2008, 54(6): 301-312.
[11]
Sweigard J A,Chumley F G, Valent B. Cloning and analysis of CUT1,a cutinase gene from Magnaporthe grisea. Mol Gen Genet, 1992,232: 174-182.
[12]
Lu J P, Feng X X, Liu X H, et al. Mnh6, a nonhistone protein, is required for fungal development and pathogenicity of Magnaporthe grisea. Fungal Genet Biol,2007, 44(9): 819-829.
[13]
Skamnioti P, Gurr S J. Magnaporthe grisea Cutinase2 mediates appressorium differentiation and host penetration and is required for full virulence. Plant Cell, 2007, 19: 2674-2689.
[14]
Yi M, Park J H, Ahn J H, et al. MoSNF1 regulates sporulation and pathogenicity in the rice blast fungus Magnaporthe oryzae. Fungal Genet Biol, 2008, 45(8): 1172-1181.
[15]
Zhou J, Zheng X Z, Lan L, et al. Biochemical and molecular characterization of a putative endoglucanase in Magnaporthe grisea.Curr Genet, 2008, 53(4): 217-224.