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中国水稻科学  2011, Vol. 25 Issue (4): 452-454    DOI: 10.3969/j.issn.1001-7216.2011.04.018
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稻瘟病菌分泌蛋白质在致病过程中的作用初探
刘永锋, 陈志谊, 俞文渊, 于俊杰, 胡建坤, 刘邮洲, 聂亚锋
江苏省农业科学院 植物保护研究所, 江苏 南京210014
Primary Study on Effect of Secreted  Proteins of Magnaporthe oryzae During  Infection
LIU  Yong-Feng, CHEN  Zhi-Yi, YU  Wen-Yuan, YU  Jun-Jie, HU  Jian-Kun, LIU  You-Zhou, NIE  Ya-Feng
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
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摘要  鉴定了稻瘟病菌孢子萌发过程中分泌产生的蛋白质。将稻瘟病菌菌株200550、200581未萌发孢子、萌发孢子以及含有孢子萌发液的萌发孢子分别接种后,病情指数分别为11.13, 35.06, 49.01和49.6, 8.4, 62.7;说明孢子萌发液中具有能够帮助稻瘟病菌侵染的物质。利用SDSPAGE电泳分析稻瘟病菌孢子萌发液,发现孢子萌发液中含有蛋白质类物质。进一步利用生物质谱鉴定和数据库资源检索,共检索到8个稻瘟病菌的蛋白质基因,其基因编号分别为MGG_01305,MGG_13188,MGG_12454等。提示这些蛋白质可能在稻瘟病菌侵染过程中具有重要的功能。
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刘永锋
陈志谊
俞文渊
于俊杰
胡建坤
刘邮洲
聂亚锋
关键词 稻瘟病菌   分泌蛋白   孢子萌发   质谱     
Abstract: Secreted proteins during spore germination of Magnaporthe oryzaewere identified. Ungerminated spores, germinated spores and the mixture of germinated spores with  germination liquid of M. oryzae (200550, 200581) were inoculated on rice seedlings, respectively. The disease indices were 1113, 3506, 4901 and 4901, 84, 627, indicating that germination liquid played a driving role in infection process.  SDSPAGE amplification revealed that secreted proteins were contained.  According to the results of mass spectrometer, and database query, eight genes were involved, including MGG_01305, MGG_13188, MGG_12454, etc. The results indicated that these proteins played key roles and important function during M. oryzae infection.
Key wordsMagnaporthe oryzae   secreted    protein   spore germination   mass spectrometer   
收稿日期: 2010-01-12;
基金资助:

江苏省农业科学院院基金资助项目(人才项目6510803)。

引用本文:   
刘永锋,陈志谊,俞文渊等. 稻瘟病菌分泌蛋白质在致病过程中的作用初探[J]. 中国水稻科学, 2011, 25(4): 452-454.
LIU Yong-Feng,CHEN Zhi-Yi,YU Wen-Yuan et al. Primary Study on Effect of Secreted  Proteins of Magnaporthe oryzae During  Infection[J]. , 2011, 25(4): 452-454.
 
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