稻瘟病菌分泌蛋白质在致病过程中的作用初探

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摘要鉴定了稻瘟病菌孢子萌发过程中分泌产生的蛋白质。将稻瘟病菌菌株200550、200581未萌发孢子、萌发孢子以及含有孢子萌发液的萌发孢子分别接种后，病情指数分别为11.13, 35.06, 49.01和49.6, 8.4, 62.7；说明孢子萌发液中具有能够帮助稻瘟病菌侵染的物质。利用SDSPAGE电泳分析稻瘟病菌孢子萌发液，发现孢子萌发液中含有蛋白质类物质。进一步利用生物质谱鉴定和数据库资源检索，共检索到8个稻瘟病菌的蛋白质基因，其基因编号分别为MGG_01305，MGG_13188，MGG_12454等。提示这些蛋白质可能在稻瘟病菌侵染过程中具有重要的功能。

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	刘永锋
	陈志谊
	俞文渊
	于俊杰
	胡建坤
	刘邮洲
	聂亚锋

关键词：稻瘟病菌分泌蛋白孢子萌发质谱

Abstract： Secreted proteins during spore germination of Magnaporthe oryzaewere identified. Ungerminated spores, germinated spores and the mixture of germinated spores with germination liquid of M. oryzae (200550, 200581) were inoculated on rice seedlings, respectively. The disease indices were 1113, 3506, 4901 and 4901, 84, 627, indicating that germination liquid played a driving role in infection process. SDSPAGE amplification revealed that secreted proteins were contained. According to the results of mass spectrometer, and database query, eight genes were involved, including MGG_01305, MGG_13188, MGG_12454, etc. The results indicated that these proteins played key roles and important function during M. oryzae infection.

Key words： Magnaporthe oryzae secreted protein spore germination mass spectrometer

收稿日期: 2010-01-12;

基金资助:

江苏省农业科学院院基金资助项目（人才项目6510803）。

引用本文:

刘永锋,陈志谊,俞文渊等. 稻瘟病菌分泌蛋白质在致病过程中的作用初探[J]. 中国水稻科学, 2011, 25(4): 452-454.

LIU Yong-Feng,CHEN Zhi-Yi,YU Wen-Yuan et al. Primary Study on Effect of Secreted Proteins of Magnaporthe oryzae During Infection[J]. , 2011, 25(4): 452-454.

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