水稻苗期氮素吸收及其相关性状的QTL分析

doi:10.3969/j.issn.1001-7216.2010.05.003

摘要/Abstract

摘要： 以来自于Koshihikari/Kasalath//Koshihikari的回交重组自交系群体为材料，采用流动液体培养和同位素示踪技术，对水稻苗期氮素吸收速率及其相关性状进行QTL分析。结果表明，地上部干质量（SW）、根系干质量（RW）、根冠比（R/S）、地上部相对生长速率（RGRS）和根相对生长速率（RGRR）等与氮素吸收力（NAA）和氮素吸收速率(NAR)密切相关。在SW、RW、植株含氮量、NAA、高氮下的氮吸收速率（HNAE）和低氮下的氮吸收速率（LNAE）上分别检测到1、4、1、3、1和1个主效QTL，主要分布在第6、7、8和10染色体上；R/S、RGRS和RGRR没有检测到主效QTL；NAA、LNAE和RW间存在明显的QTL一因多效或邻近表达现象。

关键词: 水稻, 苗期, 氮素吸收, 数量性状基因座, 回交重组自交系

Abstract: QTL analysis was done on nitrogen uptake rate and its related traits of rice at the seedling stage with the BIL population derived from Koshihikari/Kasalath//Koshihikari as materials, by the method of hydroponic and isotopic tracer. Shoot dry weight (SW), root dry weight (RW), the ratio of root dry weight to shoot dry weight (R/S), relative growth rate of shoot (RGRS) and relative growth rate of root (RGRR) were closely correlated with nitrogen absorption ability (NAA) and nitrogen absorption rate (NAR). The putative QTLs, including one for SW, four for RW, one for plant nitrogen content, three for NAA, one for HNAE(nitrogen absorption rate at high N level) and one for LNAE(nitrogen absorption rate at low N level), were detected and mainly mapped on chromosomes 6, 7, 8 and 10. There existed significant pleiotropic effects or neighboring expression between the QTLs for NAA, LNAE and RW.

Key words: rice, seedling stage, nitrogen absorption, quantitative trait locus, backcross inbred lines

王彦荣,代贵金,大杉立, 山岸辙,程式华. 水稻苗期氮素吸收及其相关性状的QTL分析[J]. 中国水稻科学 2010,24(5): 463-468 . , DOI: 10.3969/j.issn.1001-7216.2010.05.003 .

WANG Yan-rong,#,DAI Gui-jin,#,Ryu OHSUGI,Tohuru YAMAGISHI,CHENG Shi-hua. QTL Analysis on Nitrogen Uptake and Related Traits at Seedling Stage in Rice[J]. Chinese Journal of Rice Science 2010,24(5): 463-468 ., DOI: 10.3969/j.issn.1001-7216.2010.05.003 .

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