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中国水稻科学  2010, Vol. 24 Issue (5): 447-452     DOI: 10.3969/j.issn.1001-7216.2010.05.001
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转菰候选基因克隆获得抗白叶枯病水稻植株
沈玮玮1,3;宋成丽1;陈洁2;付亚萍2;吴建利2,*;江绍玫3,*
1江西师范大学 生命科学学院, 江西 南昌 330022; 2中国水稻研究所, 浙江 杭州 310006; 3江西财经大学, 江西 南昌 330013; *通讯联系人, E-mail: beishangd@163.com; shmjiang111@yahoo.com.cn
Transgenic Rice Plants Harboring Genomic DNA from Zizania latifolia Confer Bacterial Blight Resistance
SHEN Wei-wei1,3;SONG Cheng-li1;CHEN Jie2;FU Ya-ping2; WU Jian-li2,*; JIANG Shao-mei3,*
1College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China; 2 China National Rice Research Institute, Hangzhou 310006, China; 3Jiangxi University of Finance and Economics, Nanchang 330013, China; *Corresponding authors, E-mail: beishangd@163.com; shmjiang111@yahoo.com.cn
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摘要 以菰NBS-LRR类抗病基因同源序列FZ14(GenBank登录号:DQ239432)为模板设计特异性引物FZ14P1/P2 ,通过克隆池PCR法经分级筛选,从菰基因组TAC文库中获得1个阳性克隆(ZR1),序列分析比对证实该阳性克隆为含有菰抗病基因同源序列FZ14的抗病基因候选克隆。同时,ZR1具有植物NBSLRR类型抗性基因中的Ploop (kinase 1a )、 kinase 2、kinase 3a和GLPL(Gly-Leu-Pro-Leu)等保守基序,可能为抗性基因的部分序列。通过农杆菌介导转化水稻品种日本晴,获得36个对白叶枯病菌PXO71具有明显抗性的独立转化子,结果表明,菰ZR1克隆中至少含有1个白叶枯病抗性基因。
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沈玮玮
宋成丽
陈洁
付亚萍
吴建利
江绍玫
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Abstract: Based on the sequence of resistance gene analog FZ14 derived from Zizania latifolia(Griseb.), a pair of specific PCR primers FZ14P1/FZ14P2 was designed to isolate candidate disease resistance gene. The pooledPCR approach was adapted using the primer pairs to screen a genomic transformationcompetent artificial chromosome(TAC) library derived from Zizania latifolia. A positive TAC clone (ZR1) was obtained and confirmed by sequence analysis. The results indicated that ZR1 consisted of conserved motifs similar to Ploop (kinase 1a), kinase 2, kinase 3a and GLPL(Gly-Leu-Pro-Leu), indicating that it could be a portion of NBSLRR type of resistance gene. Using Agrobacterium tumafaciensmediated transformation of Nipponbare mature embryo, a total of 48 independent transgenic T0 plants were obtained. Among them, 36 plants were highly resistant to the virulent bacterial strain PXO71. The results indicated that ZR1 contains at least one bacterial blight resistance gene.
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收稿日期: 1900-01-01;
引用本文:   
沈玮玮,宋成丽,陈洁等. 转菰候选基因克隆获得抗白叶枯病水稻植株 [J]. 中国水稻科学, 2010, 24(5): 447-452 .
SHEN Wei-wei,SONG Cheng-li,CHEN Jie et al. Transgenic Rice Plants Harboring Genomic DNA from Zizania latifolia Confer Bacterial Blight Resistance[J]. , 2010, 24(5): 447-452 .
 
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