1College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China; 2 China National Rice Research Institute, Hangzhou 310006, China; 3Jiangxi University of Finance and Economics, Nanchang 330013, China; *Corresponding authors, E-mail: firstname.lastname@example.org; email@example.com
Based on the sequence of resistance gene analog FZ14 derived from Zizania latifolia(Griseb.), a pair of specific PCR primers FZ14P1/FZ14P2 was designed to isolate candidate disease resistance gene. The pooledPCR approach was adapted using the primer pairs to screen a genomic transformationcompetent artificial chromosome(TAC) library derived from Zizania latifolia. A positive TAC clone (ZR1) was obtained and confirmed by sequence analysis. The results indicated that ZR1 consisted of conserved motifs similar to Ploop (kinase 1a), kinase 2, kinase 3a and GLPL(Gly-Leu-Pro-Leu), indicating that it could be a portion of NBSLRR type of resistance gene. Using Agrobacterium tumafaciensmediated transformation of Nipponbare mature embryo, a total of 48 independent transgenic T0 plants were obtained. Among them, 36 plants were highly resistant to the virulent bacterial strain PXO71. The results indicated that ZR1 contains at least one bacterial blight resistance gene.
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