单粒稻米直链淀粉含量测定方法的研究

中国水稻科学

单粒稻米直链淀粉含量测定方法的研究

罗玉坤; 申岳正*; 闵绍楷; 熊振民

中国水稻研究所; *中国农业科学院作物育种栽培研究所

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1988-11-20 发布日期:1988-11-20

A Simplified Method on Amylose Content Determination of Single Rice Grain

Luo Yukun; Shen Yuezheng; Min Shaokai; Xiong Zhenmin

Received:1900-01-01 Revised:1900-01-01 Online:1988-11-20 Published:1988-11-20

摘要/Abstract

摘要： 本文提出一种米粒不经磨粉、煮沸,直接用冷碱液在30℃下糊化溶解后测定其直链淀粉含量的单粒稻米直链淀粉含量测定法。本法测得的结果与常规方法基本一致,并具有简便易行,工作量小,样品用量少等优点,可应用于稻米直链淀粉的生理和遗传方面的研究,为优质育种的后代筛选提供有效手段。

关键词: 水稻籽粒, 直链淀粉含量测定, 冷碱糊化

Abstract: A simplified method on amylose content determination of single rice grain, without grinding and boiling, but directly gelatinized with cold alkali soultion at 30℃, was presented. For the list, a single milled rice grain was put into a 20ml graduated tube, add 0.2 ml of 95% ethanol and 1 ml of 1.8M NaOH solution, kept in incubator for 24 hr at 30℃. After the sample shaken with vibrator, it was made up to full volume with distilled water for colorimetric assay with Autoanalyzer. The amylose content was calculated based on dry weight of single rice grain. The results was closely agreed with that from the standard method. This method has the advantages such as smaller sample, simpler procedure and easier operation, and may be used for physiological and genetical studies of amylose content of rice grain and for the screening of breeding materials.

Key words: Rice grain, Amylose content determination, Cold alkali gelatinization

罗玉坤, 申岳正, 闵绍楷, 熊振民. 单粒稻米直链淀粉含量测定方法的研究[J]. 中国水稻科学 1988,2(3): 136-140 . .

Luo Yukun,Shen Yuezheng,Min Shaokai,Xiong Zhenmin. A Simplified Method on Amylose Content Determination of Single Rice Grain[J]. Chinese Journal of Rice Science 1988,2(3): 136-140 ..