中国水稻科学

中国水稻科学

• 研究报告 •    下一篇

水稻醇溶蛋白的多态性研究

凌建群；何祖华；马志超；李德葆   

  1. 浙江农业大学生物技术研究所，杭州 310029
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1997-07-10 发布日期:1997-07-10

Studies on Polymorphism of Rice Prolamines

Ling Jianqun; He Zuhua; Ma Zhichao; Li Debao   

  • Received:1900-01-01 Revised:1900-01-01 Online:1997-07-10 Published:1997-07-10

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被引次数

50

摘要： 对40个水稻品种的醇溶蛋白进行SDS-聚丙烯酰胺凝胶电泳分析及对醇溶蛋白四组分的薄层扫描相对定量，发现水稻醇溶蛋白在蛋白组成上存在较大的多态性，基因型差异不仅可导致组分的缺失，还可影响醇溶蛋白的总量和各组分的百分含量。13kDa组分与10kDa组分，13a组分与13b组分的百分含量变化可能有一定关联；16kDa组分相对比较稳定。这表明种子发育过程中，醇溶蛋白累积调控的机制是相当复杂的。

关键词: 醇溶蛋白, 组分, 多态性, 醇溶蛋白含量

Abstract: Rice prolamines of 40 lines were studied with SDS-PAGE, and relative quantities of 16kDa, 13a, 13b and 10kDa composition were analysed by TLC scanning. The results showed that there were obvious polymorphism of prolamine composition among the lines studied; Difference of genotypes of the lines not only resulted in the absence of one or two prolamines composition, but also influence the percentages of the four composition, and the total prolamine quantities. It also appeared that 13kDa and 10kDa prolamines had some relationship in their contents as in the case of 13a and 13b; Compared with 13kDa and 10kDa prolamines, 16kDa was relatively stable in its content.These evidences suggest that the regulation mechanism of the rice prolamines accumulation would be very complicated during the seed developing.

Key words: composition, polymorphism, prolamine, prolamine quantities