中国水稻科学

中国水稻科学

• 实验技术 • 上一篇    下一篇

稻曲病菌分离技术的初探

周永力;章 琦   

  1. 中国农业科学院作物育种栽培研究所，北京 100081
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-07-10 发布日期:1999-07-10

Preliminary Report on the Isolation Technique of Ustilagonoidea virens (Cooke) Tak

Zhou Yongli; Zhang Qi   

  • Received:1900-01-01 Revised:1900-01-01 Online:1999-07-10 Published:1999-07-10

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被引次数

89

摘要： 用7种培养基和2种分离方法对稻曲病菌的培养及分离技术进行了研究。结果表明，胁本哲氏培养+抑制细菌生长的氯霉素(50 μg/mL)是分离稻曲病菌最适宜的培养基。稻谷是厚垣孢子产生的理想培养基。厚垣孢子悬液法可用于稻曲病菌的快速分离，而稻曲球不同层次组织块分离法可从保存长时间的稻曲球分离到稻曲病菌。

关键词: 稻曲病菌, 分离技术, 培养基, 菌落, 孢子

Abstract: Effect of the kinds of media, isolation methods and the stored periods of false smut ball on the isolation of Ustilagonoidea virens have been primarily studied to seek ideal isolation technique. The resuIts showed Wakimoto Toceshi+chloromycetin (50 μg /mL) is an ideal medium for the pathogen isolation, and rice grain is the best medium for the sporulation of chlamycladospores so far studied. The methods of chlamycladosporal suspension can be used for the rapid isolation of the pathogen, and the inner tissue can successfully isolate the pathogen from false smut balls which have been stored for a longtime (10 months).

Key words: culture media, colony, isolation techniques, spores, Ustilagonoidea virens