中国水稻科学

中国水稻科学

• 研究快报 •    下一篇

农杆菌介导将雪莲凝集素(GNA)基因转入籼稻单倍体微芽的初步研究

杨长登 1;唐克轩 2;吴连斌 1;李 瑶 2;赵成章 1;刘光杰 1;沈大棱 2   

  1. 1 中国水稻研究所，杭州 310006； 2 复旦大学遗传研究所, 上海 200433
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-07-10 发布日期:1998-07-10

Transformation of Haploid Indica Rice Shoots with Snowdrop Lectin Gene(GNA) by Agrobacterium-Mediated

Yang Changdeng; Tang Kexuan; Wu Lianbin; Li Yao; Zhao Chengzhang; Liu Guangjie; Shen Daleng   

  • Received:1900-01-01 Revised:1900-01-01 Online:1998-07-10 Published:1998-07-10

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摘要： 以含有双元载体(携带GNA基因，nptⅡ基因)的根癌农杆菌菌系LBA4404转化籼稻单倍体无性系微芽Hu18，经共培养后在含G418的保存培养基中连续筛选G418抗性芽，抗性芽经生根培养基中壮苗获得单倍体转化植株。PCR分析证明GNA基因已进入到Hu18细胞中，抗虫鉴定表明转基因植株具有白背飞虱抗性。并探明了G418对单倍体微芽的致死浓度和时间，共培养时间对G418抗性芽产生的影响。 

关键词: 单倍体, 无性系, 根癌农杆菌, 遗传转化, 雪莲凝集素基因

Abstract: The haploid indica rice shoots from 15-day-old in subculture were infected with Agrobacterium strain LBA4404 that contained snowdrop lectin gene(GNA) and npt-Ⅱ gene. G418-resistant (G418R) shoots weredirectly produced from shoots on shoot reproduction medium supplemented with G418 and Cefotax. Shoots were cultured on rooting medium, that resulted in G418R plantlets production. PCR analysis verified that GNA gene had already been in the cell of transgenic plants. The evaluation on resistance to brown planthopper(BPH) indicated that the transgenic plantlets have the resistance to BPH. Effect of G418 on the haploid shoots and duration of co-culturetime on G418R production were also analysed.

Key words: Agrobacterium tumefaciens, genetic transformation, GNA gene, haploid, somaclonal line